Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia
ABSTRACT The causative agents of recurrent Escherichia coli bacteremia can be genetically identical or discordant, but the differences between them remain unclear. This study aimed to explore these differences, with regard to their clinical and microbiological features. Patients were recruited from...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2021-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/Spectrum.01399-21 |
| _version_ | 1818744586310254592 |
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| author | Tatsuya Kobayashi Mahoko Ikeda Yuta Okada Yoshimi Higurashi Shu Okugawa Kyoji Moriya |
| author_facet | Tatsuya Kobayashi Mahoko Ikeda Yuta Okada Yoshimi Higurashi Shu Okugawa Kyoji Moriya |
| author_sort | Tatsuya Kobayashi |
| collection | DOAJ |
| description | ABSTRACT The causative agents of recurrent Escherichia coli bacteremia can be genetically identical or discordant, but the differences between them remain unclear. This study aimed to explore these differences, with regard to their clinical and microbiological features. Patients were recruited from a Japanese tertiary teaching hospital based on blood culture data and the incidence of recurrent E. coli bacteremia. We compared the patients' clinical and microbiological characteristics between the two groups (those with identical or discordant E. coli bacteremia) divided by the result of enterobacterial repetitive intergenic consensus PCR. Among 70 pairs of recurrent E. coli bacteremia strains, 49 pairs (70%) were genetically identical. Patients with genetically identical or discordant E. coli bacteremia were more likely to have renal failure or neoplasms, respectively. The virulence factor (VF) scores of genetically identical E. coli strains were significantly higher than those of genetically discordant strains, with the prevalence of eight VF genes being significantly higher in genetically identical E. coli strains. No significant differences were found between the two groups regarding antimicrobial susceptibility and biofilm formation potential. This study showed that genetically identical E. coli bacteremia strains have more VF genes than genetically discordant strains in recurrent E. coli bacteremia. IMPORTANCE Escherichia coli causes bloodstream infection, although not all strains are pathogenic to humans. In some cases, this infection reoccurs, and several reports have described the clinical characteristics and/or molecular microbiology of recurrent Escherichia coli bacteremia. However, these studies focused on patients with specific characteristics, and they included cases caused by microorganisms other than Escherichia coli. Hence, little is known about the pathogenicity of Escherichia coli isolated from the recurrent one. The significance of our study is in evaluating the largest cohorts to date, as no cohort studies have been conducted on this topic. |
| first_indexed | 2024-12-18T02:46:40Z |
| format | Article |
| id | doaj.art-e1b48385e28644a89686d02f67e37291 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-12-18T02:46:40Z |
| publishDate | 2021-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-e1b48385e28644a89686d02f67e372912022-12-21T21:23:33ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972021-12-019310.1128/Spectrum.01399-21Clinical and Microbiological Characteristics of Recurrent Escherichia coli BacteremiaTatsuya Kobayashi0Mahoko Ikeda1Yuta Okada2Yoshimi Higurashi3Shu Okugawa4Kyoji Moriya5Department of Infectious Diseases, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infectious Diseases, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infectious Diseases, The University of Tokyo Hospital, Tokyo, JapanDepartment of Infectious Diseases, The University of Tokyo Hospital, Tokyo, JapanABSTRACT The causative agents of recurrent Escherichia coli bacteremia can be genetically identical or discordant, but the differences between them remain unclear. This study aimed to explore these differences, with regard to their clinical and microbiological features. Patients were recruited from a Japanese tertiary teaching hospital based on blood culture data and the incidence of recurrent E. coli bacteremia. We compared the patients' clinical and microbiological characteristics between the two groups (those with identical or discordant E. coli bacteremia) divided by the result of enterobacterial repetitive intergenic consensus PCR. Among 70 pairs of recurrent E. coli bacteremia strains, 49 pairs (70%) were genetically identical. Patients with genetically identical or discordant E. coli bacteremia were more likely to have renal failure or neoplasms, respectively. The virulence factor (VF) scores of genetically identical E. coli strains were significantly higher than those of genetically discordant strains, with the prevalence of eight VF genes being significantly higher in genetically identical E. coli strains. No significant differences were found between the two groups regarding antimicrobial susceptibility and biofilm formation potential. This study showed that genetically identical E. coli bacteremia strains have more VF genes than genetically discordant strains in recurrent E. coli bacteremia. IMPORTANCE Escherichia coli causes bloodstream infection, although not all strains are pathogenic to humans. In some cases, this infection reoccurs, and several reports have described the clinical characteristics and/or molecular microbiology of recurrent Escherichia coli bacteremia. However, these studies focused on patients with specific characteristics, and they included cases caused by microorganisms other than Escherichia coli. Hence, little is known about the pathogenicity of Escherichia coli isolated from the recurrent one. The significance of our study is in evaluating the largest cohorts to date, as no cohort studies have been conducted on this topic.https://journals.asm.org/doi/10.1128/Spectrum.01399-21recurrent Escherichia coli bacteremiaenterobacterial repetitive intergenic consensus polymerase chain reactiongenetic identityvirulence factor |
| spellingShingle | Tatsuya Kobayashi Mahoko Ikeda Yuta Okada Yoshimi Higurashi Shu Okugawa Kyoji Moriya Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia Microbiology Spectrum recurrent Escherichia coli bacteremia enterobacterial repetitive intergenic consensus polymerase chain reaction genetic identity virulence factor |
| title | Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia |
| title_full | Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia |
| title_fullStr | Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia |
| title_full_unstemmed | Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia |
| title_short | Clinical and Microbiological Characteristics of Recurrent Escherichia coli Bacteremia |
| title_sort | clinical and microbiological characteristics of recurrent escherichia coli bacteremia |
| topic | recurrent Escherichia coli bacteremia enterobacterial repetitive intergenic consensus polymerase chain reaction genetic identity virulence factor |
| url | https://journals.asm.org/doi/10.1128/Spectrum.01399-21 |
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