An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.

Enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. Development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. Many researchers aim to id...

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Main Authors: Zhongchun Tong, Yuejiao Zhang, Junqi Ling, Jinglei Ma, Lijia Huang, Luodan Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089209&type=printable
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author Zhongchun Tong
Yuejiao Zhang
Junqi Ling
Jinglei Ma
Lijia Huang
Luodan Zhang
author_facet Zhongchun Tong
Yuejiao Zhang
Junqi Ling
Jinglei Ma
Lijia Huang
Luodan Zhang
author_sort Zhongchun Tong
collection DOAJ
description Enterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. Development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. Many researchers aim to identify strategies to effectively kill this drug-resistant pathogen. Here, we evaluated the effect of the antimicrobial peptide nisin on the antibacterial activities of 18 antibiotics against E. faecalis. The MIC and MBC results showed that the antibacterial activities of 18 antibiotics against E. faecalis OG1RF, ATCC 29212, and strain E were significantly improved in the presence of 200 U/ml nisin. Statistically significant differences were observed between the results with and without 200 U/ml nisin at the same concentrations of penicillin or chloramphenicol (p<0.05). The checkerboard assay showed that the combination of nisin and penicillin or chloramphenicol had a synergetic effect against the three tested E. faecalis strains. The transmission electron microscope images showed that E. faecalis was not obviously destroyed by penicillin or chloramphenicol alone but was severely disrupted by either antibiotic in combination with nisin. Furthermore, assessing biofilms by a confocal laser scanning microscope showed that penicillin, ciprofloxacin, and chloramphenicol all showed stronger antibiofilm actions in combination with nisin than when these antibiotics were administered alone. Therefore, nisin can significantly improve the antibacterial and antibiofilm activities of many antibiotics, and certain antibiotics in combination with nisin have considerable potential for use as inhibitors of this drug-resistant pathogen.
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spelling doaj.art-e1bfe94e8a054ee8be7c212defe66e902025-02-21T05:38:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8920910.1371/journal.pone.0089209An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.Zhongchun TongYuejiao ZhangJunqi LingJinglei MaLijia HuangLuodan ZhangEnterococcus faecalis rank among the leading causes of nosocomial infections worldwide and possesses both intrinsic and acquired resistance to a variety of antibiotics. Development of new antibiotics is limited, and pathogens continually generate new antibiotic resistance. Many researchers aim to identify strategies to effectively kill this drug-resistant pathogen. Here, we evaluated the effect of the antimicrobial peptide nisin on the antibacterial activities of 18 antibiotics against E. faecalis. The MIC and MBC results showed that the antibacterial activities of 18 antibiotics against E. faecalis OG1RF, ATCC 29212, and strain E were significantly improved in the presence of 200 U/ml nisin. Statistically significant differences were observed between the results with and without 200 U/ml nisin at the same concentrations of penicillin or chloramphenicol (p<0.05). The checkerboard assay showed that the combination of nisin and penicillin or chloramphenicol had a synergetic effect against the three tested E. faecalis strains. The transmission electron microscope images showed that E. faecalis was not obviously destroyed by penicillin or chloramphenicol alone but was severely disrupted by either antibiotic in combination with nisin. Furthermore, assessing biofilms by a confocal laser scanning microscope showed that penicillin, ciprofloxacin, and chloramphenicol all showed stronger antibiofilm actions in combination with nisin than when these antibiotics were administered alone. Therefore, nisin can significantly improve the antibacterial and antibiofilm activities of many antibiotics, and certain antibiotics in combination with nisin have considerable potential for use as inhibitors of this drug-resistant pathogen.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089209&type=printable
spellingShingle Zhongchun Tong
Yuejiao Zhang
Junqi Ling
Jinglei Ma
Lijia Huang
Luodan Zhang
An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
PLoS ONE
title An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
title_full An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
title_fullStr An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
title_full_unstemmed An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
title_short An in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against Enterococcus faecalis.
title_sort in vitro study on the effects of nisin on the antibacterial activities of 18 antibiotics against enterococcus faecalis
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0089209&type=printable
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