Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk

Bovine mastitis is a major disease affecting dairy cattle worldwide. The milk collected from cows with different type of inflammation, including mastitis, is characterized by an increased number of somatic cells (SCC), especially polymorphonuclear cells (PMN). It was proved that the antibacterial ac...

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Main Authors: S. Andrei, S. Matei, D. Rugină, L. Bogdan, C. Ştefănuţ
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2016-09-01
Series:Czech Journal of Animal Science
Subjects:
Online Access:https://cjas.agriculturejournals.cz/artkey/cjs-201609-0003_interrelationships-between-the-content-of-oxidative-markers-antioxidative-status-and-somatic-cell-count-in-co.php
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author S. Andrei
S. Matei
D. Rugină
L. Bogdan
C. Ştefănuţ
author_facet S. Andrei
S. Matei
D. Rugină
L. Bogdan
C. Ştefănuţ
author_sort S. Andrei
collection DOAJ
description Bovine mastitis is a major disease affecting dairy cattle worldwide. The milk collected from cows with different type of inflammation, including mastitis, is characterized by an increased number of somatic cells (SCC), especially polymorphonuclear cells (PMN). It was proved that the antibacterial activity of PMN generates reactive oxygen species and nitric oxide-derived metabolites. The accumulation of the reactive species correlating with a decrease in the antioxidant activity specific for milk can lead to oxidative and nitrosative stress. The aim of this study was to evaluate the antioxidant/oxidant status in normal cow's milk and in subclinical mastitis milk using three parameters: total antioxidant status (TAS); levels of malondialdehyde (MDA) - a degradation product of lipid peroxidation; and levels of proteins oxidation expressed as carbonylated proteins analyzed in the reaction with dinitrophenilhydrazine (DNPH). Subclinical mastitis was diagnosed using an electrical conductivity method and by determining the number of SCC in milk. Comparative analysis of TAS in normal and mastitis milk revealed extremely significant differences (P = 0.0005), the average of this parameter was lower for mastitis milk samples. The results describing the antioxidant status were correlated with those on lipid and protein oxidative degradation. The average level of MDA in mastitis milk was higher compared with normal milk, the mean difference was 40.69 nmol/ml. A similar variation was observed for DNPH, the mean difference was 191.24 µmol/ml. The levels of SCC, MDA, and DNPH were significantly higher in subclinical mastitis milk compared to milk from healthy cows due to the occurrence of oxidative stress state in subclinically inflamed mammary gland.
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spelling doaj.art-e1c035d0e5be48d093bad35108603cef2023-02-23T03:33:24ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092016-09-0161940741310.17221/70/2015-CJAScjs-201609-0003Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milkS. Andrei0S. Matei1D. Rugină2L. Bogdan3C. Ştefănuţ4Department of Biochemistry, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaDepartment of Nutrition, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaDepartment of Biochemistry, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaDepartment of Reproduction, Obstetrics and Veterinary Gynecology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaDepartment of Physiology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaBovine mastitis is a major disease affecting dairy cattle worldwide. The milk collected from cows with different type of inflammation, including mastitis, is characterized by an increased number of somatic cells (SCC), especially polymorphonuclear cells (PMN). It was proved that the antibacterial activity of PMN generates reactive oxygen species and nitric oxide-derived metabolites. The accumulation of the reactive species correlating with a decrease in the antioxidant activity specific for milk can lead to oxidative and nitrosative stress. The aim of this study was to evaluate the antioxidant/oxidant status in normal cow's milk and in subclinical mastitis milk using three parameters: total antioxidant status (TAS); levels of malondialdehyde (MDA) - a degradation product of lipid peroxidation; and levels of proteins oxidation expressed as carbonylated proteins analyzed in the reaction with dinitrophenilhydrazine (DNPH). Subclinical mastitis was diagnosed using an electrical conductivity method and by determining the number of SCC in milk. Comparative analysis of TAS in normal and mastitis milk revealed extremely significant differences (P = 0.0005), the average of this parameter was lower for mastitis milk samples. The results describing the antioxidant status were correlated with those on lipid and protein oxidative degradation. The average level of MDA in mastitis milk was higher compared with normal milk, the mean difference was 40.69 nmol/ml. A similar variation was observed for DNPH, the mean difference was 191.24 µmol/ml. The levels of SCC, MDA, and DNPH were significantly higher in subclinical mastitis milk compared to milk from healthy cows due to the occurrence of oxidative stress state in subclinically inflamed mammary gland.https://cjas.agriculturejournals.cz/artkey/cjs-201609-0003_interrelationships-between-the-content-of-oxidative-markers-antioxidative-status-and-somatic-cell-count-in-co.phpmilkmastitistotal antioxidant statusmalondialdehydeprotein oxidation
spellingShingle S. Andrei
S. Matei
D. Rugină
L. Bogdan
C. Ştefănuţ
Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
Czech Journal of Animal Science
milk
mastitis
total antioxidant status
malondialdehyde
protein oxidation
title Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
title_full Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
title_fullStr Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
title_full_unstemmed Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
title_short Interrelationships between the content of oxidative markers, antioxidative status, and somatic cell count in cow's milk
title_sort interrelationships between the content of oxidative markers antioxidative status and somatic cell count in cow s milk
topic milk
mastitis
total antioxidant status
malondialdehyde
protein oxidation
url https://cjas.agriculturejournals.cz/artkey/cjs-201609-0003_interrelationships-between-the-content-of-oxidative-markers-antioxidative-status-and-somatic-cell-count-in-co.php
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AT lbogdan interrelationshipsbetweenthecontentofoxidativemarkersantioxidativestatusandsomaticcellcountincowsmilk
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