Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells
A small organic molecule <b>P</b> was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg<sup>2+</sup>. The sensing characteristics of the proposed probe for Hg<sup>2+</sup> were studied in detail. A fluorescent enhancing prope...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/11/3385 |
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| author | Wenting Zhang Chunwei Yu Mei Yang Shaobai Wen Jun Zhang |
| author_facet | Wenting Zhang Chunwei Yu Mei Yang Shaobai Wen Jun Zhang |
| author_sort | Wenting Zhang |
| collection | DOAJ |
| description | A small organic molecule <b>P</b> was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg<sup>2+</sup>. The sensing characteristics of the proposed probe for Hg<sup>2+</sup> were studied in detail. A fluorescent enhancing property at 583 nm (>30 fold) accompanied with a visible colorimetric change, from colorless to pink, was observed with the addition of Hg<sup>2+</sup> to <b>P</b> in an ethanol-water solution (8:2, <i>v</i>/<i>v</i>, 20 mM HEPES, pH 7.0), which would be helpful to fabricate Hg<sup>2+</sup>-selective probes with “naked-eye” and fluorescent detection. Meanwhile, cellular experimental results demonstrated its low cytotoxicity and good biocompatibility, and the application of <b>P</b> for imaging of Hg<sup>2+</sup> in living cells was satisfactory. |
| first_indexed | 2024-03-10T10:45:20Z |
| format | Article |
| id | doaj.art-e1c0ba0a7015455783398f25a80770c4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:45:20Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-e1c0ba0a7015455783398f25a80770c42023-11-21T22:40:39ZengMDPI AGMolecules1420-30492021-06-012611338510.3390/molecules26113385Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living CellsWenting Zhang0Chunwei Yu1Mei Yang2Shaobai Wen3Jun Zhang4Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, ChinaLaboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, ChinaSchool of Public Health, Hainan Medical University, Haikou 571101, ChinaLaboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, ChinaLaboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, ChinaA small organic molecule <b>P</b> was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg<sup>2+</sup>. The sensing characteristics of the proposed probe for Hg<sup>2+</sup> were studied in detail. A fluorescent enhancing property at 583 nm (>30 fold) accompanied with a visible colorimetric change, from colorless to pink, was observed with the addition of Hg<sup>2+</sup> to <b>P</b> in an ethanol-water solution (8:2, <i>v</i>/<i>v</i>, 20 mM HEPES, pH 7.0), which would be helpful to fabricate Hg<sup>2+</sup>-selective probes with “naked-eye” and fluorescent detection. Meanwhile, cellular experimental results demonstrated its low cytotoxicity and good biocompatibility, and the application of <b>P</b> for imaging of Hg<sup>2+</sup> in living cells was satisfactory.https://www.mdpi.com/1420-3049/26/11/3385Hg<sup>2+</sup>fluorescent proberhodamine Bcell imaging |
| spellingShingle | Wenting Zhang Chunwei Yu Mei Yang Shaobai Wen Jun Zhang Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells Molecules Hg<sup>2+</sup> fluorescent probe rhodamine B cell imaging |
| title | Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
| title_full | Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
| title_fullStr | Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
| title_full_unstemmed | Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
| title_short | Characterization of a Hg<sup>2+</sup>-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
| title_sort | characterization of a hg sup 2 sup selective fluorescent probe based on rhodamine b and its imaging in living cells |
| topic | Hg<sup>2+</sup> fluorescent probe rhodamine B cell imaging |
| url | https://www.mdpi.com/1420-3049/26/11/3385 |
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