Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares
In some species, catecholamines in follicular fluid (FF) are related to local physiological events responsible for the regulation of ovarian functions and oocyte maturation. The aim of the present study was to determine and compare intrafollicular and systemic concentrations of dopamine (DA), noradr...
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1896 |
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| author | Katiuska Satué Esterina Fazio Maria Dolores Rubio Cristina Cravana Pietro Medica |
| author_facet | Katiuska Satué Esterina Fazio Maria Dolores Rubio Cristina Cravana Pietro Medica |
| author_sort | Katiuska Satué |
| collection | DOAJ |
| description | In some species, catecholamines in follicular fluid (FF) are related to local physiological events responsible for the regulation of ovarian functions and oocyte maturation. The aim of the present study was to determine and compare intrafollicular and systemic concentrations of dopamine (DA), noradrenaline (NA) and adrenaline (AD) in cycling mares. Sixty ovaries were collected during breeding season from 30 mares raised for slaughterhouse meat production, with clinically normal reproductive tracts, were evaluated. Blood samples were collected prior to slaughter. Follicles were classified into three categories in relation to size: small (20–30 mm; <i>n</i> = 20), medium (≥31–40 mm; <i>n</i> = 20) and large (≥41 mm; <i>n</i> = 20). Follicular fluid (FF) samples were extracted from each follicle. Intrafollicular DA, NA and AD concentrations were significantly higher than the systemic concentrations (<i>p</i> < 0.05). Intrafollicular DA concentrations were higher in medium than small and large follicles (<i>p</i> < 0.05). Intrafollicular NA concentrations were higher in small than medium and large follicles (<i>p</i> < 0.05). Intrafollicular AD concentrations were higher in large than small and medium follicles (<i>p</i> < 0.05). Follicle diameter was significantly and negatively correlated with NA and AD (<i>p</i> < 0.05). A significant correlation of the same hormone concentration in FF and in systemic fluid was observed (<i>p</i> < 0.05). In summary, the FF can serve as an intraovarian catecholamine-storing compartment, with the ability to release neurotransmitters in a regulated way. These results provide novel insights into the neuronal nature of the follicle, suggesting the involvement of catecholamines in normal ovarian functions in mares. |
| first_indexed | 2024-03-10T15:34:59Z |
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| language | English |
| last_indexed | 2024-03-10T15:34:59Z |
| publishDate | 2020-10-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-e1c24a153c8d463aaaeb4e65a283769d2023-11-20T17:20:43ZengMDPI AGAnimals2076-26152020-10-011010189610.3390/ani10101896Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling MaresKatiuska Satué0Esterina Fazio1Maria Dolores Rubio2Cristina Cravana3Pietro Medica4Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, CEU-Cardenal Herrera University, Tirant lo Blanc, 7, Alfara del Patriarca, 46115 Valencia, SpainDepartment of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Via Palatucci, 98168 Messina, ItalyDepartment of Cellular Biology, Physiology and Immunology, Faculty of Veterinary, University of Córdoba, Campus of Rabanales, 14071 Córdoba, SpainDepartment of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Via Palatucci, 98168 Messina, ItalyDepartment of Veterinary Sciences, Veterinary Physiology Unit, Messina University, Via Palatucci, 98168 Messina, ItalyIn some species, catecholamines in follicular fluid (FF) are related to local physiological events responsible for the regulation of ovarian functions and oocyte maturation. The aim of the present study was to determine and compare intrafollicular and systemic concentrations of dopamine (DA), noradrenaline (NA) and adrenaline (AD) in cycling mares. Sixty ovaries were collected during breeding season from 30 mares raised for slaughterhouse meat production, with clinically normal reproductive tracts, were evaluated. Blood samples were collected prior to slaughter. Follicles were classified into three categories in relation to size: small (20–30 mm; <i>n</i> = 20), medium (≥31–40 mm; <i>n</i> = 20) and large (≥41 mm; <i>n</i> = 20). Follicular fluid (FF) samples were extracted from each follicle. Intrafollicular DA, NA and AD concentrations were significantly higher than the systemic concentrations (<i>p</i> < 0.05). Intrafollicular DA concentrations were higher in medium than small and large follicles (<i>p</i> < 0.05). Intrafollicular NA concentrations were higher in small than medium and large follicles (<i>p</i> < 0.05). Intrafollicular AD concentrations were higher in large than small and medium follicles (<i>p</i> < 0.05). Follicle diameter was significantly and negatively correlated with NA and AD (<i>p</i> < 0.05). A significant correlation of the same hormone concentration in FF and in systemic fluid was observed (<i>p</i> < 0.05). In summary, the FF can serve as an intraovarian catecholamine-storing compartment, with the ability to release neurotransmitters in a regulated way. These results provide novel insights into the neuronal nature of the follicle, suggesting the involvement of catecholamines in normal ovarian functions in mares.https://www.mdpi.com/2076-2615/10/10/1896adrenalinecycling maredopaminefollicular fluidnoradrenaline |
| spellingShingle | Katiuska Satué Esterina Fazio Maria Dolores Rubio Cristina Cravana Pietro Medica Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares Animals adrenaline cycling mare dopamine follicular fluid noradrenaline |
| title | Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares |
| title_full | Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares |
| title_fullStr | Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares |
| title_full_unstemmed | Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares |
| title_short | Intrafollicular and Systemic Dopamine, Noradrenaline and Adrenaline Concentrations in Cycling Mares |
| title_sort | intrafollicular and systemic dopamine noradrenaline and adrenaline concentrations in cycling mares |
| topic | adrenaline cycling mare dopamine follicular fluid noradrenaline |
| url | https://www.mdpi.com/2076-2615/10/10/1896 |
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