Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics
Abstract As one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor,...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-023-09382-6 |
| _version_ | 1797811995541504000 |
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| author | Yingke Liu Yilin Wei Yaqing Dou Chenlei Li Chenglei Song Zhe Zhang Kunlong Qi Xinjian Li Ruimin Qiao Kejun Wang Xiuling Li Feng Yang Xuelei Han |
| author_facet | Yingke Liu Yilin Wei Yaqing Dou Chenlei Li Chenglei Song Zhe Zhang Kunlong Qi Xinjian Li Ruimin Qiao Kejun Wang Xiuling Li Feng Yang Xuelei Han |
| author_sort | Yingke Liu |
| collection | DOAJ |
| description | Abstract As one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor, tenderness, and juiciness of pork. Therefore, it is of great significance to study the mechanism of porcine IMF deposition. Previous research indicated that miR-149-5p promoted the proliferation of porcine intramuscular (IM) preadipocytes and decreased their ability to differentiate, albeit the exact mechanism of action is unknown. In vitro, foreign pigs showed increased miR-149-5p expression and reduced fat deposition when compared to Queshan Black pigs. This study conducted metabolomics and transcriptomics analyses of porcine IM preadipocytes overexpressing miR-149-5p to verify their effects on lipid formation. According to metabolomics analysis, the overexpression of miR-149-5p has significantly altered the lipid, organic acid, and organic oxygen metabolites of porcine IM preadipocytes. Specially speaking, it has changed 115 metabolites, including 105 up-regulated and 10 down-regulated ones, as well as the composition of lipid, organic acid, and organic oxygen metabolism-related metabolites. RNA-seq analysis showed that overexpression of miR-149-5p significantly altered 857 genes, of which 442 were up-regulated, and 415 were down-regulated, with enrichment to MAPK, IL-17, PI3K-Akt, and ErbB signaling pathways. We found that overexpression of miR-149-5p inhibited adipogenic differentiation by changing cAMP signaling pathway in porcine IM preadipocytes. In addition, the overexpression of miR-149-5p may affect the transport of Cu2+ by targeting ATP7A and inhibiting adipogenic differentiation. These findings elucidate the regulatory function of miR-149-5p in porcine IM preadipocytes, which may be a key target for controlling pork quality. |
| first_indexed | 2024-03-13T07:30:57Z |
| format | Article |
| id | doaj.art-e1c76b812143468e8af55788c0694749 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-13T07:30:57Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-e1c76b812143468e8af55788c06947492023-06-04T11:07:18ZengBMCBMC Genomics1471-21642023-05-0124111510.1186/s12864-023-09382-6Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomicsYingke Liu0Yilin Wei1Yaqing Dou2Chenlei Li3Chenglei Song4Zhe Zhang5Kunlong Qi6Xinjian Li7Ruimin Qiao8Kejun Wang9Xiuling Li10Feng Yang11Xuelei Han12College of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityCollege of Animal Science and Technology, Henan Agricultural UniversityAbstract As one of the important traits in pig production, meat quality has important research significance and value. Intramuscular fat (IMF) content is one of the most important factors affecting pork quality. Many experimental studies have shown that IMF content is closely related to the flavor, tenderness, and juiciness of pork. Therefore, it is of great significance to study the mechanism of porcine IMF deposition. Previous research indicated that miR-149-5p promoted the proliferation of porcine intramuscular (IM) preadipocytes and decreased their ability to differentiate, albeit the exact mechanism of action is unknown. In vitro, foreign pigs showed increased miR-149-5p expression and reduced fat deposition when compared to Queshan Black pigs. This study conducted metabolomics and transcriptomics analyses of porcine IM preadipocytes overexpressing miR-149-5p to verify their effects on lipid formation. According to metabolomics analysis, the overexpression of miR-149-5p has significantly altered the lipid, organic acid, and organic oxygen metabolites of porcine IM preadipocytes. Specially speaking, it has changed 115 metabolites, including 105 up-regulated and 10 down-regulated ones, as well as the composition of lipid, organic acid, and organic oxygen metabolism-related metabolites. RNA-seq analysis showed that overexpression of miR-149-5p significantly altered 857 genes, of which 442 were up-regulated, and 415 were down-regulated, with enrichment to MAPK, IL-17, PI3K-Akt, and ErbB signaling pathways. We found that overexpression of miR-149-5p inhibited adipogenic differentiation by changing cAMP signaling pathway in porcine IM preadipocytes. In addition, the overexpression of miR-149-5p may affect the transport of Cu2+ by targeting ATP7A and inhibiting adipogenic differentiation. These findings elucidate the regulatory function of miR-149-5p in porcine IM preadipocytes, which may be a key target for controlling pork quality.https://doi.org/10.1186/s12864-023-09382-6miR-149-5pTranscriptomicsMetabolomicsATP7APorcine intramuscular preadipocytes |
| spellingShingle | Yingke Liu Yilin Wei Yaqing Dou Chenlei Li Chenglei Song Zhe Zhang Kunlong Qi Xinjian Li Ruimin Qiao Kejun Wang Xiuling Li Feng Yang Xuelei Han Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics BMC Genomics miR-149-5p Transcriptomics Metabolomics ATP7A Porcine intramuscular preadipocytes |
| title | Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| title_full | Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| title_fullStr | Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| title_full_unstemmed | Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| title_short | Effect of miR-149-5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| title_sort | effect of mir 149 5p on intramuscular fat deposition in pigs based on metabolomics and transcriptomics |
| topic | miR-149-5p Transcriptomics Metabolomics ATP7A Porcine intramuscular preadipocytes |
| url | https://doi.org/10.1186/s12864-023-09382-6 |
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