Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>)
Tilapia, as one of the fish widely cultured around the world, is suffering severe impact from the <i>streptococcus</i> disease with the deterioration of the breeding environment and the increasing of breeding density, which brings serious economic loss to tilapia farming. In this study,...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/7/1262 |
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| author | Ye Cao Jia Liu Gaoyang Liu Hui Du Tianqiang Liu Gaoxue Wang Qing Wang Ya Zhou Erlong Wang |
| author_facet | Ye Cao Jia Liu Gaoyang Liu Hui Du Tianqiang Liu Gaoxue Wang Qing Wang Ya Zhou Erlong Wang |
| author_sort | Ye Cao |
| collection | DOAJ |
| description | Tilapia, as one of the fish widely cultured around the world, is suffering severe impact from the <i>streptococcus</i> disease with the deterioration of the breeding environment and the increasing of breeding density, which brings serious economic loss to tilapia farming. In this study, the surface immunogenic protein (Sip) of <i>Streptococcus agalactiae</i> (<i>S. agalactiae</i>) was selected as the potential candidate antigen and connected with bacterial nano cellulose (BNC) to construct the nanocarrier subunit vaccine (BNC-rSip), and the immersion immune effects against <i>S. agalactiae</i> and <i>Streptococcus iniae</i> (<i>S. iniae</i>) in Nile tilapia were evaluated on the basis of the serum antibody level, non-specific enzyme activity, the immune-related gene expression and relative percent survival (RPS). The results indicated that Sip possessed the expected immunogenicity according to the immunoinformatic analysis. Compared with the rSip group, BNC-rSip significantly induced serum antibody production and improved the innate immunity level of tilapia. After challenge, the RPS of BNC-rSip groups were 78.95% (<i>S. agalactiae</i>) and 67.86% (<i>S. iniae</i>), which were both higher than those of rSip groups,31.58% (<i>S. agalactiae</i>) and 35.71% (<i>S. iniae</i>), respectively. Our study indicated that BNC-rSip can induce protective immunity for tilapia through immersion immunization and may be an ideal candidate vaccine for controlling tilapia streptococcal disease. |
| first_indexed | 2024-03-11T00:34:42Z |
| format | Article |
| id | doaj.art-e1c8b0db26c44c8da6a93ed577ebd1e9 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T00:34:42Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-e1c8b0db26c44c8da6a93ed577ebd1e92023-11-18T21:41:53ZengMDPI AGVaccines2076-393X2023-07-01117126210.3390/vaccines11071262Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>)Ye Cao0Jia Liu1Gaoyang Liu2Hui Du3Tianqiang Liu4Gaoxue Wang5Qing Wang6Ya Zhou7Erlong Wang8Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaKey Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, ChinaCollege of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404155, ChinaNorthwest A&F University Shenzhen Research Institute, Shenzhen 518000, ChinaTilapia, as one of the fish widely cultured around the world, is suffering severe impact from the <i>streptococcus</i> disease with the deterioration of the breeding environment and the increasing of breeding density, which brings serious economic loss to tilapia farming. In this study, the surface immunogenic protein (Sip) of <i>Streptococcus agalactiae</i> (<i>S. agalactiae</i>) was selected as the potential candidate antigen and connected with bacterial nano cellulose (BNC) to construct the nanocarrier subunit vaccine (BNC-rSip), and the immersion immune effects against <i>S. agalactiae</i> and <i>Streptococcus iniae</i> (<i>S. iniae</i>) in Nile tilapia were evaluated on the basis of the serum antibody level, non-specific enzyme activity, the immune-related gene expression and relative percent survival (RPS). The results indicated that Sip possessed the expected immunogenicity according to the immunoinformatic analysis. Compared with the rSip group, BNC-rSip significantly induced serum antibody production and improved the innate immunity level of tilapia. After challenge, the RPS of BNC-rSip groups were 78.95% (<i>S. agalactiae</i>) and 67.86% (<i>S. iniae</i>), which were both higher than those of rSip groups,31.58% (<i>S. agalactiae</i>) and 35.71% (<i>S. iniae</i>), respectively. Our study indicated that BNC-rSip can induce protective immunity for tilapia through immersion immunization and may be an ideal candidate vaccine for controlling tilapia streptococcal disease.https://www.mdpi.com/2076-393X/11/7/1262<i>Streptococcus</i>surface immunogenic proteinimmersion immunizationTilapia |
| spellingShingle | Ye Cao Jia Liu Gaoyang Liu Hui Du Tianqiang Liu Gaoxue Wang Qing Wang Ya Zhou Erlong Wang Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) Vaccines <i>Streptococcus</i> surface immunogenic protein immersion immunization Tilapia |
| title | Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) |
| title_full | Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) |
| title_fullStr | Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) |
| title_full_unstemmed | Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) |
| title_short | Exploring the Immunoprotective Potential of a Nanocarrier Immersion Vaccine Encoding Sip against <i>Streptococcus</i> Infection in Tilapia (<i>Oreochromis niloticus</i>) |
| title_sort | exploring the immunoprotective potential of a nanocarrier immersion vaccine encoding sip against i streptococcus i infection in tilapia i oreochromis niloticus i |
| topic | <i>Streptococcus</i> surface immunogenic protein immersion immunization Tilapia |
| url | https://www.mdpi.com/2076-393X/11/7/1262 |
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