Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization
Two simple and sensitive spectrophotometric methods are proposed for the determination of amitriptyline in its pure form and in tablets. The first method is based on the formation of charge- transfer complex between amitriptyline as n-donor and tetracyano-ethylene (TCNE) as πacceptor. The product...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2017-07-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/1123 |
| _version_ | 1828287074943369216 |
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| author | A. k . Mohammed |
| author_facet | A. k . Mohammed |
| author_sort | A. k . Mohammed |
| collection | DOAJ |
| description |
Two simple and sensitive spectrophotometric methods are proposed for the determination of amitriptyline in its pure form and in tablets. The first method is based on the formation of charge- transfer complex between amitriptyline as n-donor and tetracyano-ethylene (TCNE) as πacceptor. The product exhibit absorbance maximum at 470 nm in acetonitrile solvent (pH =9.0 ) . In the second method the absorbance of the ion- pair complex, which is formed between the soughted drug and bromocresol green (BCG), was measured at 415 nm at ( pH=3.5) . In addition to classical univariate optimization, modified simplex method (MSM) was applied in the optimization of the variable affecting the color producing reaction by a geometric simplex in three dimensions of space. Beers, law was obeyed in the concentration ranges 6.0-70 and 8.0-100 µg.ml-1 with molar absorbitivites of 2275 and 1475 l. mol-1 cm-1 for TCNE and BCG methods respectively. The limits of detection of the two methods are 0.043 and 0.034 µg.ml-1 and their Sandells sensitivity values are 0.122 and 0.188 µg.ml-1 respectively.
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| first_indexed | 2024-04-13T09:42:22Z |
| format | Article |
| id | doaj.art-e1ca46f2bc1c428fb1c91a0b7bc144a3 |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-04-13T09:42:22Z |
| publishDate | 2017-07-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-e1ca46f2bc1c428fb1c91a0b7bc144a32022-12-22T02:51:53ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072017-07-01224Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex OptimizationA. k . Mohammed Two simple and sensitive spectrophotometric methods are proposed for the determination of amitriptyline in its pure form and in tablets. The first method is based on the formation of charge- transfer complex between amitriptyline as n-donor and tetracyano-ethylene (TCNE) as πacceptor. The product exhibit absorbance maximum at 470 nm in acetonitrile solvent (pH =9.0 ) . In the second method the absorbance of the ion- pair complex, which is formed between the soughted drug and bromocresol green (BCG), was measured at 415 nm at ( pH=3.5) . In addition to classical univariate optimization, modified simplex method (MSM) was applied in the optimization of the variable affecting the color producing reaction by a geometric simplex in three dimensions of space. Beers, law was obeyed in the concentration ranges 6.0-70 and 8.0-100 µg.ml-1 with molar absorbitivites of 2275 and 1475 l. mol-1 cm-1 for TCNE and BCG methods respectively. The limits of detection of the two methods are 0.043 and 0.034 µg.ml-1 and their Sandells sensitivity values are 0.122 and 0.188 µg.ml-1 respectively. https://jih.uobaghdad.edu.iq/index.php/j/article/view/1123 |
| spellingShingle | A. k . Mohammed Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization Ibn Al-Haitham Journal for Pure and Applied Sciences |
| title | Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization |
| title_full | Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization |
| title_fullStr | Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization |
| title_full_unstemmed | Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization |
| title_short | Development of Two New Spectrophotometeric Methods for the Determination of Amitriptyline in Pharmaceutical Preparation Using Univariate and Simplex Optimization |
| title_sort | development of two new spectrophotometeric methods for the determination of amitriptyline in pharmaceutical preparation using univariate and simplex optimization |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/1123 |
| work_keys_str_mv | AT akmohammed developmentoftwonewspectrophotometericmethodsforthedeterminationofamitriptylineinpharmaceuticalpreparationusingunivariateandsimplexoptimization |