Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice
The disease of type 2 diabetes mellitus (T2DM) is principally induced by insufficient insulin secretion and insulin resistance. In the current study, Sanghuangporus vaninii fruit body polysaccharide (SVP) was prepared and structurally characterized. It was shown that the yield of SVP was 1.91%, and...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1013466/full |
| _version_ | 1797986131761954816 |
|---|---|
| author | Zi-Rui Huang Qi-Zhen Huang Ke-Wen Chen Zi-Feng Huang Yun Liu Rui-Bo Jia Bin Liu Bin Liu |
| author_facet | Zi-Rui Huang Qi-Zhen Huang Ke-Wen Chen Zi-Feng Huang Yun Liu Rui-Bo Jia Bin Liu Bin Liu |
| author_sort | Zi-Rui Huang |
| collection | DOAJ |
| description | The disease of type 2 diabetes mellitus (T2DM) is principally induced by insufficient insulin secretion and insulin resistance. In the current study, Sanghuangporus vaninii fruit body polysaccharide (SVP) was prepared and structurally characterized. It was shown that the yield of SVP was 1.91%, and SVP mainly contains small molecular weight polysaccharides. Afterward, the hypoglycemic and hypolipidemic effects and the potential mechanism of SVP in T2DM mice were investigated. The results exhibited oral SVP could reverse the body weight loss, high levels of blood glucose, insulin resistance, hyperlipidemia, and inflammation in T2DM mice. Oral SVP increased fecal short-chain fatty acids (SCFAs) concentrations of T2DM mice. Additionally, 16S rRNA sequencing analysis illustrated that SVP can modulate the structure and function of intestinal microflora in T2DM mice, indicating as decreasing the levels of Firmicutes/Bacteroidetes, Flavonifractor, Odoribacter, and increasing the levels of Weissella, Alloprevotella, and Dubosiella. Additionally, the levels of predicted metabolic functions of Citrate cycle, GABAergic synapse, Insulin signaling pathway were increased, and those of Purine metabolism, Taurine and hypotaurine metabolism, and Starch and sucrose metabolism were decreased in intestinal microflora after SVP treatment. These findings demonstrate that SVP could potentially play hypoglycemic and hypolipidemic effects by regulating gut microflora and be a promising nutraceutical for ameliorating T2DM. |
| first_indexed | 2024-04-11T07:29:10Z |
| format | Article |
| id | doaj.art-e1cb1bb4587e4a1d83666f524130a562 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T07:29:10Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-e1cb1bb4587e4a1d83666f524130a5622022-12-22T04:36:59ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-10-01910.3389/fnut.2022.10134661013466Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic miceZi-Rui Huang0Qi-Zhen Huang1Ke-Wen Chen2Zi-Feng Huang3Yun Liu4Rui-Bo Jia5Bin Liu6Bin Liu7College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaChaozhou Branch of Chemistry and Chemical Engineering Guangdong Laboratory, Chaozhou, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaNational Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, ChinaThe disease of type 2 diabetes mellitus (T2DM) is principally induced by insufficient insulin secretion and insulin resistance. In the current study, Sanghuangporus vaninii fruit body polysaccharide (SVP) was prepared and structurally characterized. It was shown that the yield of SVP was 1.91%, and SVP mainly contains small molecular weight polysaccharides. Afterward, the hypoglycemic and hypolipidemic effects and the potential mechanism of SVP in T2DM mice were investigated. The results exhibited oral SVP could reverse the body weight loss, high levels of blood glucose, insulin resistance, hyperlipidemia, and inflammation in T2DM mice. Oral SVP increased fecal short-chain fatty acids (SCFAs) concentrations of T2DM mice. Additionally, 16S rRNA sequencing analysis illustrated that SVP can modulate the structure and function of intestinal microflora in T2DM mice, indicating as decreasing the levels of Firmicutes/Bacteroidetes, Flavonifractor, Odoribacter, and increasing the levels of Weissella, Alloprevotella, and Dubosiella. Additionally, the levels of predicted metabolic functions of Citrate cycle, GABAergic synapse, Insulin signaling pathway were increased, and those of Purine metabolism, Taurine and hypotaurine metabolism, and Starch and sucrose metabolism were decreased in intestinal microflora after SVP treatment. These findings demonstrate that SVP could potentially play hypoglycemic and hypolipidemic effects by regulating gut microflora and be a promising nutraceutical for ameliorating T2DM.https://www.frontiersin.org/articles/10.3389/fnut.2022.1013466/fullSanghuangporus vaniniipolysaccharidehypoglycemic and hypolipidemic activityinflammationintestinal microflora |
| spellingShingle | Zi-Rui Huang Qi-Zhen Huang Ke-Wen Chen Zi-Feng Huang Yun Liu Rui-Bo Jia Bin Liu Bin Liu Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice Frontiers in Nutrition Sanghuangporus vaninii polysaccharide hypoglycemic and hypolipidemic activity inflammation intestinal microflora |
| title | Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| title_full | Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| title_fullStr | Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| title_full_unstemmed | Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| title_short | Sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| title_sort | sanghuangporus vaninii fruit body polysaccharide alleviates hyperglycemia and hyperlipidemia via modulating intestinal microflora in type 2 diabetic mice |
| topic | Sanghuangporus vaninii polysaccharide hypoglycemic and hypolipidemic activity inflammation intestinal microflora |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1013466/full |
| work_keys_str_mv | AT ziruihuang sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT qizhenhuang sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT kewenchen sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT zifenghuang sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT yunliu sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT ruibojia sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT binliu sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice AT binliu sanghuangporusvaniniifruitbodypolysaccharidealleviateshyperglycemiaandhyperlipidemiaviamodulatingintestinalmicrofloraintype2diabeticmice |