Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>)
We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/13/9/2307 |
| _version_ | 1797581679389310976 |
|---|---|
| author | Louise Winther Søren Kjærsgaard Rasmussen Gert Poulsen Conny Bruun Asmussen Lange |
| author_facet | Louise Winther Søren Kjærsgaard Rasmussen Gert Poulsen Conny Bruun Asmussen Lange |
| author_sort | Louise Winther |
| collection | DOAJ |
| description | We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (<i>PIC</i>) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding histories. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over the years and accessions, respectively. The mean thousand seed weight was 152.05 g. More than 10 accessions had a protein content above 28%, showing that the collection has potential as breeding nursery for high-protein pea cultivars. |
| first_indexed | 2024-03-10T23:08:57Z |
| format | Article |
| id | doaj.art-e1cc7faf45b046519b3e7152b9cf3d85 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:08:57Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-e1cc7faf45b046519b3e7152b9cf3d852023-11-19T09:10:27ZengMDPI AGAgronomy2073-43952023-08-01139230710.3390/agronomy13092307Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>)Louise Winther0Søren Kjærsgaard Rasmussen1Gert Poulsen2Conny Bruun Asmussen Lange3Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Copenhagen, DenmarkDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Copenhagen, DenmarkDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Copenhagen, DenmarkDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Copenhagen, DenmarkWe produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (<i>PIC</i>) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding histories. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over the years and accessions, respectively. The mean thousand seed weight was 152.05 g. More than 10 accessions had a protein content above 28%, showing that the collection has potential as breeding nursery for high-protein pea cultivars.https://www.mdpi.com/2073-4395/13/9/2307pea<i>Pisum sativum</i> L.SSR markers<i>PIC</i>microsatellitesgenetic diversity |
| spellingShingle | Louise Winther Søren Kjærsgaard Rasmussen Gert Poulsen Conny Bruun Asmussen Lange Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) Agronomy pea <i>Pisum sativum</i> L. SSR markers <i>PIC</i> microsatellites genetic diversity |
| title | Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) |
| title_full | Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) |
| title_fullStr | Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) |
| title_full_unstemmed | Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) |
| title_short | Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (<i>Pisum sativum</i>) |
| title_sort | assessment of genetic diversity and protein content of scandinavian peas i pisum sativum i |
| topic | pea <i>Pisum sativum</i> L. SSR markers <i>PIC</i> microsatellites genetic diversity |
| url | https://www.mdpi.com/2073-4395/13/9/2307 |
| work_keys_str_mv | AT louisewinther assessmentofgeneticdiversityandproteincontentofscandinavianpeasipisumsativumi AT sørenkjærsgaardrasmussen assessmentofgeneticdiversityandproteincontentofscandinavianpeasipisumsativumi AT gertpoulsen assessmentofgeneticdiversityandproteincontentofscandinavianpeasipisumsativumi AT connybruunasmussenlange assessmentofgeneticdiversityandproteincontentofscandinavianpeasipisumsativumi |