Genotyping of Brazilian Giardia duodenalis human axenic isolates
Giardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G), but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SciELO
2011-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300016 |
| _version_ | 1828864709256806400 |
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| author | ST Coradi EB David TCG Oliveira-Sequeira PEM Ribolla TB Carvalho S Guimarães |
| author_facet | ST Coradi EB David TCG Oliveira-Sequeira PEM Ribolla TB Carvalho S Guimarães |
| author_sort | ST Coradi |
| collection | DOAJ |
| description | Giardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G), but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were not genetically typed yet. So, in the present study, five Brazilian axenic isolates obtained from asymptomatic and symptomatic patients were typed in order to determine the major genetic groups to which the isolates belonged. DNA was extracted from axenic trophozoites, fragments of glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes were amplified by PCR and the isolate genotyping was carried out using restriction fragment length polymorphism (RFLP) and DNA sequencing for both genes. The results revealed that all isolates were assigned to genotype A at both analyzed loci. Indeed, DNA sequence analysis classified the four isolates obtained from asymptomatic individuals into subtype AII, while the isolate obtained from the symptomatic patient was typed as subtype AI. Despite of the limited number of isolates assessed, the findings presented herein provide interesting insights on the occurrence of Giardia genotypes in Brazil and hold the perspective for future molecular and epidemiological investigations. |
| first_indexed | 2024-12-13T04:13:14Z |
| format | Article |
| id | doaj.art-e1d34dc0e0014c768b3f63e8c6e5b35d |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-13T04:13:14Z |
| publishDate | 2011-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-e1d34dc0e0014c768b3f63e8c6e5b35d2022-12-21T23:59:57ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992011-01-0117335335710.1590/S1678-91992011000300016Genotyping of Brazilian Giardia duodenalis human axenic isolatesST CoradiEB DavidTCG Oliveira-SequeiraPEM RibollaTB CarvalhoS GuimarãesGiardia duodenalis is a complex species that comprises at least seven distinct genetic groups (A to G), but only genotypes A and B are known to infect humans and a wide variety of other mammals. Regardless of biological, biochemical and antigenic analysis, several isolates maintained in vitro were not genetically typed yet. So, in the present study, five Brazilian axenic isolates obtained from asymptomatic and symptomatic patients were typed in order to determine the major genetic groups to which the isolates belonged. DNA was extracted from axenic trophozoites, fragments of glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi) genes were amplified by PCR and the isolate genotyping was carried out using restriction fragment length polymorphism (RFLP) and DNA sequencing for both genes. The results revealed that all isolates were assigned to genotype A at both analyzed loci. Indeed, DNA sequence analysis classified the four isolates obtained from asymptomatic individuals into subtype AII, while the isolate obtained from the symptomatic patient was typed as subtype AI. Despite of the limited number of isolates assessed, the findings presented herein provide interesting insights on the occurrence of Giardia genotypes in Brazil and hold the perspective for future molecular and epidemiological investigations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300016Giardia duodenalisaxenic isolatesmolecular typinggenotype |
| spellingShingle | ST Coradi EB David TCG Oliveira-Sequeira PEM Ribolla TB Carvalho S Guimarães Genotyping of Brazilian Giardia duodenalis human axenic isolates Journal of Venomous Animals and Toxins including Tropical Diseases Giardia duodenalis axenic isolates molecular typing genotype |
| title | Genotyping of Brazilian Giardia duodenalis human axenic isolates |
| title_full | Genotyping of Brazilian Giardia duodenalis human axenic isolates |
| title_fullStr | Genotyping of Brazilian Giardia duodenalis human axenic isolates |
| title_full_unstemmed | Genotyping of Brazilian Giardia duodenalis human axenic isolates |
| title_short | Genotyping of Brazilian Giardia duodenalis human axenic isolates |
| title_sort | genotyping of brazilian giardia duodenalis human axenic isolates |
| topic | Giardia duodenalis axenic isolates molecular typing genotype |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300016 |
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