Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM
Retinol shows complex photophysical properties that make it potentially useful as an exogenous or endogenous probe of membrane microenvironment, but it has not been fully explored. In this study, we use bulk fluorescence lifetime measurements and fluorescence lifetime imaging microscopy (FLIM) to ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | BBA Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667160323000170 |
| _version_ | 1827919332433199104 |
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| author | L. Sumrall L. Smith E. Alhatmi Y. Chmykh D. Mitchell J. Nadeau |
| author_facet | L. Sumrall L. Smith E. Alhatmi Y. Chmykh D. Mitchell J. Nadeau |
| author_sort | L. Sumrall |
| collection | DOAJ |
| description | Retinol shows complex photophysical properties that make it potentially useful as an exogenous or endogenous probe of membrane microenvironment, but it has not been fully explored. In this study, we use bulk fluorescence lifetime measurements and fluorescence lifetime imaging microscopy (FLIM) to examine the stability of retinol in phosphatidylcholine (PC) multilamellar and unilamellar vesicles with and without cholesterol. We find that both light and exposure to ambient temperature and oxygen contribute to retinol degradation, with the addition of an antioxidant such as butylated hydroxytoluene (BHT) essential to provide stability, especially in the absence of cholesterol. With exposure to ultraviolet light to excite its native fluorescence, retinol degrades rapidly and can photosensitize vesicles. Degradation can be measured by a decrease in fluorescence lifetime. In POPC vesicles without cholesterol, BHT leads to initially higher lifetimes compared with no BHT, but it increases the rate of photodegradation. The presence of 10 mol % cholesterol protects against this effect, and vesicles with 20 mol % cholesterol show longer lifetimes without BHT under all conditions. Because of its environmental sensitivity, retinol is interesting as a FLIM probe, but careful controls are needed to avoid degradation, and additional work is needed to optimize liposomes for food and cosmetic applications. |
| first_indexed | 2024-03-13T03:56:24Z |
| format | Article |
| id | doaj.art-e1d7748521024ed19188b5eaa5b0dee4 |
| institution | Directory Open Access Journal |
| issn | 2667-1603 |
| language | English |
| last_indexed | 2024-03-13T03:56:24Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | BBA Advances |
| spelling | doaj.art-e1d7748521024ed19188b5eaa5b0dee42023-06-22T05:05:54ZengElsevierBBA Advances2667-16032023-01-013100088Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIML. Sumrall0L. Smith1E. Alhatmi2Y. Chmykh3D. Mitchell4J. Nadeau5Department of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesDepartment of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesDepartment of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesDepartment of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesDepartment of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesCorresponding author.; Department of Physics, SRTC Room, Portland State University, 1719 SW 10th Ave. 134, Portland, OR 97201, United StatesRetinol shows complex photophysical properties that make it potentially useful as an exogenous or endogenous probe of membrane microenvironment, but it has not been fully explored. In this study, we use bulk fluorescence lifetime measurements and fluorescence lifetime imaging microscopy (FLIM) to examine the stability of retinol in phosphatidylcholine (PC) multilamellar and unilamellar vesicles with and without cholesterol. We find that both light and exposure to ambient temperature and oxygen contribute to retinol degradation, with the addition of an antioxidant such as butylated hydroxytoluene (BHT) essential to provide stability, especially in the absence of cholesterol. With exposure to ultraviolet light to excite its native fluorescence, retinol degrades rapidly and can photosensitize vesicles. Degradation can be measured by a decrease in fluorescence lifetime. In POPC vesicles without cholesterol, BHT leads to initially higher lifetimes compared with no BHT, but it increases the rate of photodegradation. The presence of 10 mol % cholesterol protects against this effect, and vesicles with 20 mol % cholesterol show longer lifetimes without BHT under all conditions. Because of its environmental sensitivity, retinol is interesting as a FLIM probe, but careful controls are needed to avoid degradation, and additional work is needed to optimize liposomes for food and cosmetic applications.http://www.sciencedirect.com/science/article/pii/S2667160323000170RetinolFluorescence lifetimeFLIMPhotosensitizerPhotodegradation |
| spellingShingle | L. Sumrall L. Smith E. Alhatmi Y. Chmykh D. Mitchell J. Nadeau Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM BBA Advances Retinol Fluorescence lifetime FLIM Photosensitizer Photodegradation |
| title | Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM |
| title_full | Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM |
| title_fullStr | Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM |
| title_full_unstemmed | Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM |
| title_short | Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM |
| title_sort | stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and flim |
| topic | Retinol Fluorescence lifetime FLIM Photosensitizer Photodegradation |
| url | http://www.sciencedirect.com/science/article/pii/S2667160323000170 |
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