Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2015-06-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en |
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| author | Raniere M. Moura Arthur A. Melo Rômulo F. Carneiro Cícera R.f. Rodrigues Plínio Delatorre Kyria S. Nascimento Silvana Saker-Sampaio Celso S. Nagano Benildo S. Cavada Alexandre H. Sampaio |
| author_facet | Raniere M. Moura Arthur A. Melo Rômulo F. Carneiro Cícera R.f. Rodrigues Plínio Delatorre Kyria S. Nascimento Silvana Saker-Sampaio Celso S. Nagano Benildo S. Cavada Alexandre H. Sampaio |
| author_sort | Raniere M. Moura |
| collection | DOAJ |
| description | Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. |
| first_indexed | 2024-12-20T04:13:58Z |
| format | Article |
| id | doaj.art-e1d971871071475d9bb375319feca71a |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-20T04:13:58Z |
| publishDate | 2015-06-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-e1d971871071475d9bb375319feca71a2022-12-21T19:53:50ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-06-0187297398410.1590/0001-3765201520140399S0001-37652015000200973Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria griseaRaniere M. MouraArthur A. MeloRômulo F. CarneiroCícera R.f. RodriguesPlínio DelatorreKyria S. NascimentoSilvana Saker-SampaioCelso S. NaganoBenildo S. CavadaAlexandre H. SampaioTwenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=enpurificaçãohemólisecitotoxicidadegalactose |
| spellingShingle | Raniere M. Moura Arthur A. Melo Rômulo F. Carneiro Cícera R.f. Rodrigues Plínio Delatorre Kyria S. Nascimento Silvana Saker-Sampaio Celso S. Nagano Benildo S. Cavada Alexandre H. Sampaio Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea Anais da Academia Brasileira de Ciências purificação hemólise citotoxicidade galactose |
| title | Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea |
| title_full | Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea |
| title_fullStr | Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea |
| title_full_unstemmed | Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea |
| title_short | Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea |
| title_sort | hemagglutinating hemolytic activities in extracts of marine invertebrates from the brazilian coast and isolation of two lectins from the marine sponge cliona varians and the sea cucumber holothuria grisea |
| topic | purificação hemólise citotoxicidade galactose |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en |
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