Deformation of cellular components of bone forming cells when exposed to a magnetic field
It has been suggested that alignment control of biological macromolecules and structures is possible when the diamagnetic energy is sufficient to overcome the thermal energy. Strong magnetic fields of several Tesla (T) have previously been shown to enable the observation of the magnetic orientations...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2019-03-01
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| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/1.5079635 |
| _version_ | 1831841351837155328 |
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| author | M. Iwasaka |
| author_facet | M. Iwasaka |
| author_sort | M. Iwasaka |
| collection | DOAJ |
| description | It has been suggested that alignment control of biological macromolecules and structures is possible when the diamagnetic energy is sufficient to overcome the thermal energy. Strong magnetic fields of several Tesla (T) have previously been shown to enable the observation of the magnetic orientations of protein fibers and suspended or adherent cells. In the present study, the fine structure of a bone-forming osteoblast cell line was observed in real-time under strong static magnetic fields of up to 5 T. The magnetic field was applied normal to the adhering surface, and induced slight deformation of cellular shape under bright field illumination. A newly developed dark field observation method presented more precise analysis of the inner structures of the cells in strong magnetic fields. Specifically, structures with a round appearance were clearly visualized around the cell nucleus using the electromagnetic illumination. In addition to the magnetically induced swelling of the round structures—which showed differences in diameter of several micrometers—color changes for these intracellular components were also detected using this technique. A high-resolution analysis of fine cellular structure based on the magnetic orientation of intracellular components including microtubules is proposed. |
| first_indexed | 2024-12-23T06:28:11Z |
| format | Article |
| id | doaj.art-e1dcd0065a7c48e3bf75d403b64a599d |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-12-23T06:28:11Z |
| publishDate | 2019-03-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-e1dcd0065a7c48e3bf75d403b64a599d2022-12-21T17:57:01ZengAIP Publishing LLCAIP Advances2158-32262019-03-0193035327035327-510.1063/1.5079635069992ADVDeformation of cellular components of bone forming cells when exposed to a magnetic fieldM. Iwasaka0Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashihiroshima, Hiroshima 739-8530, Japan and Research Institute for Nanodevice and Bio Systems, Hiroshima University, Higashihiroshima, Hiroshima 739-8527, JapanIt has been suggested that alignment control of biological macromolecules and structures is possible when the diamagnetic energy is sufficient to overcome the thermal energy. Strong magnetic fields of several Tesla (T) have previously been shown to enable the observation of the magnetic orientations of protein fibers and suspended or adherent cells. In the present study, the fine structure of a bone-forming osteoblast cell line was observed in real-time under strong static magnetic fields of up to 5 T. The magnetic field was applied normal to the adhering surface, and induced slight deformation of cellular shape under bright field illumination. A newly developed dark field observation method presented more precise analysis of the inner structures of the cells in strong magnetic fields. Specifically, structures with a round appearance were clearly visualized around the cell nucleus using the electromagnetic illumination. In addition to the magnetically induced swelling of the round structures—which showed differences in diameter of several micrometers—color changes for these intracellular components were also detected using this technique. A high-resolution analysis of fine cellular structure based on the magnetic orientation of intracellular components including microtubules is proposed.http://dx.doi.org/10.1063/1.5079635 |
| spellingShingle | M. Iwasaka Deformation of cellular components of bone forming cells when exposed to a magnetic field AIP Advances |
| title | Deformation of cellular components of bone forming cells when exposed to a magnetic field |
| title_full | Deformation of cellular components of bone forming cells when exposed to a magnetic field |
| title_fullStr | Deformation of cellular components of bone forming cells when exposed to a magnetic field |
| title_full_unstemmed | Deformation of cellular components of bone forming cells when exposed to a magnetic field |
| title_short | Deformation of cellular components of bone forming cells when exposed to a magnetic field |
| title_sort | deformation of cellular components of bone forming cells when exposed to a magnetic field |
| url | http://dx.doi.org/10.1063/1.5079635 |
| work_keys_str_mv | AT miwasaka deformationofcellularcomponentsofboneformingcellswhenexposedtoamagneticfield |