Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c
Multidrug-resistant bacteria have become a major threat to global public health. Bacteriophages are regarded as a promising substitute. Here, we present a novel lytic Stenotrophomonas maltophilia phage, vB_SM_ytsc_ply2008005c, which was isolated from sewage water samples in Qingdao, east China. Viri...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Virus Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170222001848 |
| _version_ | 1797366693598593024 |
|---|---|
| author | Luokai Wang Jiayue Zhang Xing Liu Houqi Ning Hong Lin Jingxue Wang |
| author_facet | Luokai Wang Jiayue Zhang Xing Liu Houqi Ning Hong Lin Jingxue Wang |
| author_sort | Luokai Wang |
| collection | DOAJ |
| description | Multidrug-resistant bacteria have become a major threat to global public health. Bacteriophages are regarded as a promising substitute. Here, we present a novel lytic Stenotrophomonas maltophilia phage, vB_SM_ytsc_ply2008005c, which was isolated from sewage water samples in Qingdao, east China. Virion morphology of phage particles indicated that ply2008005c has an icosahedral head (56 ± 5 nm in diameter) and a noncontractile sheathed tail (129 ± 6 nm in length), which are the typical characteristics of phages belonging to the family Siphoviridae. Phage ply2008005c could be used for phage therapy for its stability in a wide pH (4 to 12) range and high temperature (up to 70°C) environment. Genome analysis revealed that ply2008005c has a circular double-strand DNA of 42,318 bp with a G+C content of 63.02%. It shared the closest relationship with phage vB_PaeS_PAO1_Ab18, but the homology coverage is just 20%. There were 54 open reading frames predicted in its genome, including three unique proteins and 34 functional genes in different modules. The phylogenetic analysis revealed that ply2008005c forms a distinct branch of the family Siphoviridae. These results demonstrated that ply2008005c was supposed to be a representative new member within the family Siphoviridae, which could be considered a potential bioagent against multidrug-resistant S. maltophilia. |
| first_indexed | 2024-03-08T17:07:23Z |
| format | Article |
| id | doaj.art-e1e497e086d94dd4bbf1e98496684d66 |
| institution | Directory Open Access Journal |
| issn | 1872-7492 |
| language | English |
| last_indexed | 2024-03-08T17:07:23Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj.art-e1e497e086d94dd4bbf1e98496684d662024-01-04T04:36:05ZengElsevierVirus Research1872-74922022-09-01318198856Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005cLuokai Wang0Jiayue Zhang1Xing Liu2Houqi Ning3Hong Lin4Jingxue Wang5Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, PR ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, PR ChinaDepartment of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, PR ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, PR ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, PR ChinaFood Safety Laboratory, College of Food Science and Engineering, Ocean University of China, No. 5, Yushan Road, Qingdao, Shandong 266003, PR China; Corresponding author.Multidrug-resistant bacteria have become a major threat to global public health. Bacteriophages are regarded as a promising substitute. Here, we present a novel lytic Stenotrophomonas maltophilia phage, vB_SM_ytsc_ply2008005c, which was isolated from sewage water samples in Qingdao, east China. Virion morphology of phage particles indicated that ply2008005c has an icosahedral head (56 ± 5 nm in diameter) and a noncontractile sheathed tail (129 ± 6 nm in length), which are the typical characteristics of phages belonging to the family Siphoviridae. Phage ply2008005c could be used for phage therapy for its stability in a wide pH (4 to 12) range and high temperature (up to 70°C) environment. Genome analysis revealed that ply2008005c has a circular double-strand DNA of 42,318 bp with a G+C content of 63.02%. It shared the closest relationship with phage vB_PaeS_PAO1_Ab18, but the homology coverage is just 20%. There were 54 open reading frames predicted in its genome, including three unique proteins and 34 functional genes in different modules. The phylogenetic analysis revealed that ply2008005c forms a distinct branch of the family Siphoviridae. These results demonstrated that ply2008005c was supposed to be a representative new member within the family Siphoviridae, which could be considered a potential bioagent against multidrug-resistant S. maltophilia.http://www.sciencedirect.com/science/article/pii/S0168170222001848BacteriophageSiphoviridae phagesStenotrophomonas maltophiliaGenome analysis |
| spellingShingle | Luokai Wang Jiayue Zhang Xing Liu Houqi Ning Hong Lin Jingxue Wang Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c Virus Research Bacteriophage Siphoviridae phages Stenotrophomonas maltophilia Genome analysis |
| title | Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c |
| title_full | Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c |
| title_fullStr | Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c |
| title_full_unstemmed | Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c |
| title_short | Biological characterization and complete genome analysis of a novel Stenotrophomonas maltophilia phage vB_SM_ytsc_ply2008005c |
| title_sort | biological characterization and complete genome analysis of a novel stenotrophomonas maltophilia phage vb sm ytsc ply2008005c |
| topic | Bacteriophage Siphoviridae phages Stenotrophomonas maltophilia Genome analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0168170222001848 |
| work_keys_str_mv | AT luokaiwang biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c AT jiayuezhang biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c AT xingliu biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c AT houqining biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c AT honglin biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c AT jingxuewang biologicalcharacterizationandcompletegenomeanalysisofanovelstenotrophomonasmaltophiliaphagevbsmytscply2008005c |