Evolution of a confluent gut epithelium under on-chip cyclic stretching
The progress of food in the gastrointestinal (GI) tract is driven by a peristaltic motion generated by the muscle belt surrounding the GI tract. In turn, the response of the intestinal epithelial cells to the peristaltic stresses affects the dynamics of the epithelial structure. In this work, we stu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Physical Society
2022-04-01
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| Series: | Physical Review Research |
| Online Access: | http://doi.org/10.1103/PhysRevResearch.4.023032 |
| _version_ | 1797210823665385472 |
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| author | Lauriane Gérémie Efe Ilker Moencopi Bernheim-Dennery Charles Cavaniol Jean-Louis Viovy Danijela Matic Vignjevic Jean-François Joanny Stéphanie Descroix |
| author_facet | Lauriane Gérémie Efe Ilker Moencopi Bernheim-Dennery Charles Cavaniol Jean-Louis Viovy Danijela Matic Vignjevic Jean-François Joanny Stéphanie Descroix |
| author_sort | Lauriane Gérémie |
| collection | DOAJ |
| description | The progress of food in the gastrointestinal (GI) tract is driven by a peristaltic motion generated by the muscle belt surrounding the GI tract. In turn, the response of the intestinal epithelial cells to the peristaltic stresses affects the dynamics of the epithelial structure. In this work, we study the effect of cyclic stretching (0.125 Hz, 10% strain) on the spatial organization of the intestinal epithelium using intestinal cells deposited on a flat elastomeric substrate to mimic the peristaltic motion in vitro. A confluent monolayer of Caco-2 cells is grown on a PDMS chip to probe the morphological and orientational response of the tissue to cyclic stretching. The PDMS chips are either covalently or noncovalently coated with laminin to recapitulate the basement membrane. We observe a significant orientational response where the cells rearrange their long axes perpendicular to the stretching direction for both coating conditions. The experiment is modeled by a vertex model where the cells store elastic energy with varying strain and effectively have a rotational diffusive motion of their long axes through rearrangements of their shapes. The model also predicts a transition between the perpendicular orientation and the orientation at an oblique angle determined by the level of the cell elastic anisotropy. It provides a general framework to study cell response and relaxation dynamics under cyclic stretching across different cell types. We also discuss potential relevance of peristalsis in determining planar cell polarity in three-dimensional architectures. |
| first_indexed | 2024-04-24T10:16:43Z |
| format | Article |
| id | doaj.art-e1e6864b360a4f5cb035be1f6e447bd4 |
| institution | Directory Open Access Journal |
| issn | 2643-1564 |
| language | English |
| last_indexed | 2024-04-24T10:16:43Z |
| publishDate | 2022-04-01 |
| publisher | American Physical Society |
| record_format | Article |
| series | Physical Review Research |
| spelling | doaj.art-e1e6864b360a4f5cb035be1f6e447bd42024-04-12T17:19:46ZengAmerican Physical SocietyPhysical Review Research2643-15642022-04-014202303210.1103/PhysRevResearch.4.023032Evolution of a confluent gut epithelium under on-chip cyclic stretchingLauriane GérémieEfe IlkerMoencopi Bernheim-DenneryCharles CavaniolJean-Louis ViovyDanijela Matic VignjevicJean-François JoannyStéphanie DescroixThe progress of food in the gastrointestinal (GI) tract is driven by a peristaltic motion generated by the muscle belt surrounding the GI tract. In turn, the response of the intestinal epithelial cells to the peristaltic stresses affects the dynamics of the epithelial structure. In this work, we study the effect of cyclic stretching (0.125 Hz, 10% strain) on the spatial organization of the intestinal epithelium using intestinal cells deposited on a flat elastomeric substrate to mimic the peristaltic motion in vitro. A confluent monolayer of Caco-2 cells is grown on a PDMS chip to probe the morphological and orientational response of the tissue to cyclic stretching. The PDMS chips are either covalently or noncovalently coated with laminin to recapitulate the basement membrane. We observe a significant orientational response where the cells rearrange their long axes perpendicular to the stretching direction for both coating conditions. The experiment is modeled by a vertex model where the cells store elastic energy with varying strain and effectively have a rotational diffusive motion of their long axes through rearrangements of their shapes. The model also predicts a transition between the perpendicular orientation and the orientation at an oblique angle determined by the level of the cell elastic anisotropy. It provides a general framework to study cell response and relaxation dynamics under cyclic stretching across different cell types. We also discuss potential relevance of peristalsis in determining planar cell polarity in three-dimensional architectures.http://doi.org/10.1103/PhysRevResearch.4.023032 |
| spellingShingle | Lauriane Gérémie Efe Ilker Moencopi Bernheim-Dennery Charles Cavaniol Jean-Louis Viovy Danijela Matic Vignjevic Jean-François Joanny Stéphanie Descroix Evolution of a confluent gut epithelium under on-chip cyclic stretching Physical Review Research |
| title | Evolution of a confluent gut epithelium under on-chip cyclic stretching |
| title_full | Evolution of a confluent gut epithelium under on-chip cyclic stretching |
| title_fullStr | Evolution of a confluent gut epithelium under on-chip cyclic stretching |
| title_full_unstemmed | Evolution of a confluent gut epithelium under on-chip cyclic stretching |
| title_short | Evolution of a confluent gut epithelium under on-chip cyclic stretching |
| title_sort | evolution of a confluent gut epithelium under on chip cyclic stretching |
| url | http://doi.org/10.1103/PhysRevResearch.4.023032 |
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