Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza
Abstract Genetic components that regulate arbuscular mycorrhizal (AM) interactions in hosts and non-hosts are not completely known. Comparative transcriptomic analysis was combined with phylogenetic studies to identify the factors that distinguish AM host from non-host. Mycorrhized host, non-mycorrh...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-93626-6 |
| _version_ | 1818432573612752896 |
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| author | Shalini Vasan Divya Srivastava David Cahill Pushplata Prasad Singh Alok Adholeya |
| author_facet | Shalini Vasan Divya Srivastava David Cahill Pushplata Prasad Singh Alok Adholeya |
| author_sort | Shalini Vasan |
| collection | DOAJ |
| description | Abstract Genetic components that regulate arbuscular mycorrhizal (AM) interactions in hosts and non-hosts are not completely known. Comparative transcriptomic analysis was combined with phylogenetic studies to identify the factors that distinguish AM host from non-host. Mycorrhized host, non-mycorrhized host and non-host cultivars of tomato (Solanum lycopersicum) were subjected to RNA seq analysis. The top 10 differentially expressed genes were subjected to extensive in silico phylogenetic analysis along with 10 more candidate genes that have been previously reported for AM-plant interactions. Seven distantly related hosts and four non-hosts were selected to identify structural differences in selected gene/protein candidates. The screened genes/proteins were subjected to MEME, CODEML and DIVERGE analysis to identify evolutionary patterns that differentiate hosts from non-hosts. Based on the results, candidate genes were categorized as highly influenced (SYMRK and CCaMK), moderately influenced and minimally influenced by evolutionary constraints. We propose that the amino acid and nucleotide changes specific to non-hosts are likely to correspond to aberrations in functionality towards AM symbiosis. This study paves way for future research aimed at understanding innate differences in genetic make-up of AM hosts and non-hosts, in addition to the theory of gene losses from the “AM-symbiotic toolkit”. |
| first_indexed | 2024-12-14T16:07:21Z |
| format | Article |
| id | doaj.art-e1e689a6bedd43cbac8d19374e568a26 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:07:21Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e1e689a6bedd43cbac8d19374e568a262022-12-21T22:55:03ZengNature PortfolioScientific Reports2045-23222021-07-0111111510.1038/s41598-021-93626-6Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhizaShalini Vasan0Divya Srivastava1David Cahill2Pushplata Prasad Singh3Alok Adholeya4TERI-Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute (TERI)TERI-Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute (TERI)School of Life and Environmental Sciences, Deakin UniversityTERI-Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute (TERI)TERI-Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute (TERI)Abstract Genetic components that regulate arbuscular mycorrhizal (AM) interactions in hosts and non-hosts are not completely known. Comparative transcriptomic analysis was combined with phylogenetic studies to identify the factors that distinguish AM host from non-host. Mycorrhized host, non-mycorrhized host and non-host cultivars of tomato (Solanum lycopersicum) were subjected to RNA seq analysis. The top 10 differentially expressed genes were subjected to extensive in silico phylogenetic analysis along with 10 more candidate genes that have been previously reported for AM-plant interactions. Seven distantly related hosts and four non-hosts were selected to identify structural differences in selected gene/protein candidates. The screened genes/proteins were subjected to MEME, CODEML and DIVERGE analysis to identify evolutionary patterns that differentiate hosts from non-hosts. Based on the results, candidate genes were categorized as highly influenced (SYMRK and CCaMK), moderately influenced and minimally influenced by evolutionary constraints. We propose that the amino acid and nucleotide changes specific to non-hosts are likely to correspond to aberrations in functionality towards AM symbiosis. This study paves way for future research aimed at understanding innate differences in genetic make-up of AM hosts and non-hosts, in addition to the theory of gene losses from the “AM-symbiotic toolkit”.https://doi.org/10.1038/s41598-021-93626-6 |
| spellingShingle | Shalini Vasan Divya Srivastava David Cahill Pushplata Prasad Singh Alok Adholeya Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza Scientific Reports |
| title | Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza |
| title_full | Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza |
| title_fullStr | Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza |
| title_full_unstemmed | Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza |
| title_short | Important innate differences in determining symbiotic responsiveness in host and non-hosts of arbuscular mycorrhiza |
| title_sort | important innate differences in determining symbiotic responsiveness in host and non hosts of arbuscular mycorrhiza |
| url | https://doi.org/10.1038/s41598-021-93626-6 |
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