Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens
Introduction: Most cytology laboratories in all Gulf countries including Oman, use the standard papanicolaou (PAP) method to stain various cytological specimens. The aim of this study was to investigate the possible application of ultrafast PAP (UF‑PAP) method in cytology laboratory. Mate...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2016-01-01
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| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.176229 |
| _version_ | 1818720536078843904 |
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| author | Nasar Yousuf Alwahaibi Mariam Said Alsubhi Najat Aldairi Amna Alshukaili Usha Rani Bai |
| author_facet | Nasar Yousuf Alwahaibi Mariam Said Alsubhi Najat Aldairi Amna Alshukaili Usha Rani Bai |
| author_sort | Nasar Yousuf Alwahaibi |
| collection | DOAJ |
| description | Introduction: Most cytology laboratories in all Gulf countries including Oman, use the standard papanicolaou (PAP) method to stain various cytological specimens. The aim of this study was to investigate the possible application of ultrafast PAP (UF‑PAP) method in cytology laboratory.
Materials and Methods: Samples from 46 patients containing 26 body fluids and 20 fine needle aspirations (FNAs) (9 thyroids and 11 breasts) were collected. Two air dried and two wet smears from each sample were prepared and stained by UF‑PAP and the standard PAP stains, respectively. Background, nuclear staining, cell morphology, and overall staining were independently reviewed by two cytoscreeners.
Results: In all cases of FNA, UF-PAP stain gave a good score for the background, nuclear staining, cell morphology, and overall staining when compared with the standard PAP method. Although the correct diagnosis was made in all cases of body cavity fluids cases except in one case, UF-PAP stain gave a fewer score in the assessment of body cavity fluid samples.
Conclusion: The findings of this study support the use of UF‑PAP method in cytology laboratory with a high emphasis on FNA samples. |
| first_indexed | 2024-12-17T20:24:23Z |
| format | Article |
| id | doaj.art-e1e98a66ec2d439babc23d4f46315559 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-17T20:24:23Z |
| publishDate | 2016-01-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-e1e98a66ec2d439babc23d4f463155592022-12-21T21:33:51ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262016-01-0180101902410.4103/0974-2727.176229Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration SpecimensNasar Yousuf Alwahaibi0Mariam Said Alsubhi1Najat Aldairi2Amna Alshukaili3Usha Rani Bai4Department of Allied Health Sciences, Sultan Qaboos University, Muscat, OmanDepartment of Pathology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Pathology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Pathology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Pathology, Sultan Qaboos University Hospital, Muscat, OmanIntroduction: Most cytology laboratories in all Gulf countries including Oman, use the standard papanicolaou (PAP) method to stain various cytological specimens. The aim of this study was to investigate the possible application of ultrafast PAP (UF‑PAP) method in cytology laboratory. Materials and Methods: Samples from 46 patients containing 26 body fluids and 20 fine needle aspirations (FNAs) (9 thyroids and 11 breasts) were collected. Two air dried and two wet smears from each sample were prepared and stained by UF‑PAP and the standard PAP stains, respectively. Background, nuclear staining, cell morphology, and overall staining were independently reviewed by two cytoscreeners. Results: In all cases of FNA, UF-PAP stain gave a good score for the background, nuclear staining, cell morphology, and overall staining when compared with the standard PAP method. Although the correct diagnosis was made in all cases of body cavity fluids cases except in one case, UF-PAP stain gave a fewer score in the assessment of body cavity fluid samples. Conclusion: The findings of this study support the use of UF‑PAP method in cytology laboratory with a high emphasis on FNA samples.http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.176229body cavity fluidscytologyfine needle aspirationpapanicolaouultrafast papanicolaou |
| spellingShingle | Nasar Yousuf Alwahaibi Mariam Said Alsubhi Najat Aldairi Amna Alshukaili Usha Rani Bai Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens Journal of Laboratory Physicians body cavity fluids cytology fine needle aspiration papanicolaou ultrafast papanicolaou |
| title | Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens |
| title_full | Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens |
| title_fullStr | Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens |
| title_full_unstemmed | Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens |
| title_short | Comparison of Ultrafast Papanicolaou Stain with the Standard Papanicolaou Stain in Body Fluids and Fine Needle Aspiration Specimens |
| title_sort | comparison of ultrafast papanicolaou stain with the standard papanicolaou stain in body fluids and fine needle aspiration specimens |
| topic | body cavity fluids cytology fine needle aspiration papanicolaou ultrafast papanicolaou |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/0974-2727.176229 |
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