Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)
Coral reefs are impacted by a range of environmental variables that affect their growth and survival, the main factors being the high irradiance and temperature fluctuations. Specimens of Pocillopora capitata Verrill 1864 were exposed to photosynthetically active radiation (PAR) and ultraviolet radi...
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Vicerractoría Investigación
2010-03-01
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| Series: | Revista de Biología Tropical |
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| Online Access: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442010000100008 |
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| author | Marco A Liñán-Cabello Laura A Flores-Ramírez José Francisco Cobo-Díaz Tania Zenteno- Savin Norma O Olguín-Monroy Aramís Olivos-Ortiz Adrián Tintos-Gómez |
| author_facet | Marco A Liñán-Cabello Laura A Flores-Ramírez José Francisco Cobo-Díaz Tania Zenteno- Savin Norma O Olguín-Monroy Aramís Olivos-Ortiz Adrián Tintos-Gómez |
| author_sort | Marco A Liñán-Cabello |
| collection | DOAJ |
| description | Coral reefs are impacted by a range of environmental variables that affect their growth and survival, the main factors being the high irradiance and temperature fluctuations. Specimens of Pocillopora capitata Verrill 1864 were exposed to photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) for 32h under laboratory conditions. We examined lipid peroxidation (MDA), antioxidant enzyme activities (SOD, CAT, GPx and GST), chlorophyll a (Chl a), carotenoid pigments (CPs), mycosporine-like amino acids (MAAs), and expulsion of zooxanthellae. Our results revealed that corals exposed to UVR had relatively low levels of carotenoids and antioxidant enzyme activities compared to those exposed to PAR, as well as lower CPs/Chl a ratios. Although MAAs and CPs are rapidly produced as non-enzymatic antioxidants in response to UVR in corals, these were not sufficient, even in the dark phase of the experiment, to mitigate the damage caused by formation of reactive oxygen species (ROS), which caused breakdown of the symbiotic relationship between the zooxanthellae and the host animal to an extent 33 times greater than in the PAR treatment. In this study, it could be possible to distinguish that, parallel to the short-term adjustments, such as the amount of pigment in the algae or the sensitivity of the photosynthetic response reported in other species of coral, P. capitata exhibits at the enzymatic level a series of responses oriented to resist the effects derived from the propagation of ROS and, thus, to adapt to and maintain its reproductive capacity in shallow oceanic environments that commonly exhibit high UVR levels. Nevertheless, as a result of the inappropriate location of the artificial intercommunication structure of the Juluapan Lagoon with respect to the arrecifal area of study and therefore of the tides influence, other variables, such as the changes in short-term in turbidity, sediment inputs, nutrients, temperature and osmolarity, can act in combination and cause irreversible damage. The implementation of a management plan for the coralline reefs of the Mexican Pacific coast is required. Rev. Biol. Trop. 58 (1): 103-118. Epub 2010 March 01.<br>Los arrecifes de coral se ven afectados por una serie de variables ambientales que afectan su crecimiento y supervivencia, siendo los principales factores la alta irradiación y las fluctuaciones de temperatura. Los especímenes de Pocillopora capitata Verrill 1864 fueron expuestos a radiación activa fotosintéticamente (PAR) y radiación ultravioleta (RUV) por 32h en condiciones de laboratorio. Nosotros determinamos las concentraciones de peroxidación lipídica (MDA), actividades de enzimas antioxidantes (SOD, CAT, GPx y GST), clorofila a (Chl a), pigmentos carotenoides (CPS), aminoácidos tipo micosporina (MAAS), y la expulsión de las zooxantelas. Nuestros resultados muestran que los corales expuestos a los rayos UV presentaban niveles relativamente bajos de carotenoides y actividad de las enzimas antioxidantes en comparación con los expuestos al PAR, así como tasas de CPs/Chl a bajas. Aunque MAAs y CPs se producen rápidamente como antioxidantes no enzimáticos en respuesta a la radiación ultravioleta en los corales, éstos no fueron suficientes, incluso en la fase oscura del experimento, para mitigar los daños causados por la formación de especies reactivas de oxígeno (ROS), lo que provocó una ruptura en la relación simbiótica entre las zooxantelas y el coral con una relación 33 veces mayor que en el tratamiento de PAR. A nivel enzimático, P capitata presentó una serie de ajustes orientados a resistir los efectos derivados de la propagación de ROS y con ello favorecer su adaptación y capacidad reproductiva en ambientes oceánicos caracterizados por altos niveles de UVR. |
| first_indexed | 2024-03-12T06:22:26Z |
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| institution | Directory Open Access Journal |
| issn | 0034-7744 2215-2075 |
| language | English |
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| publishDate | 2010-03-01 |
| publisher | Vicerractoría Investigación |
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| series | Revista de Biología Tropical |
| spelling | doaj.art-e1ea9019410645d3befb813f28a982622023-09-03T02:09:20ZengVicerractoría InvestigaciónRevista de Biología Tropical0034-77442215-20752010-03-01581103118Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia)Marco A Liñán-CabelloLaura A Flores-RamírezJosé Francisco Cobo-DíazTania Zenteno- SavinNorma O Olguín-MonroyAramís Olivos-OrtizAdrián Tintos-GómezCoral reefs are impacted by a range of environmental variables that affect their growth and survival, the main factors being the high irradiance and temperature fluctuations. Specimens of Pocillopora capitata Verrill 1864 were exposed to photosynthetically active radiation (PAR) and ultraviolet radiation (UVR) for 32h under laboratory conditions. We examined lipid peroxidation (MDA), antioxidant enzyme activities (SOD, CAT, GPx and GST), chlorophyll a (Chl a), carotenoid pigments (CPs), mycosporine-like amino acids (MAAs), and expulsion of zooxanthellae. Our results revealed that corals exposed to UVR had relatively low levels of carotenoids and antioxidant enzyme activities compared to those exposed to PAR, as well as lower CPs/Chl a ratios. Although MAAs and CPs are rapidly produced as non-enzymatic antioxidants in response to UVR in corals, these were not sufficient, even in the dark phase of the experiment, to mitigate the damage caused by formation of reactive oxygen species (ROS), which caused breakdown of the symbiotic relationship between the zooxanthellae and the host animal to an extent 33 times greater than in the PAR treatment. In this study, it could be possible to distinguish that, parallel to the short-term adjustments, such as the amount of pigment in the algae or the sensitivity of the photosynthetic response reported in other species of coral, P. capitata exhibits at the enzymatic level a series of responses oriented to resist the effects derived from the propagation of ROS and, thus, to adapt to and maintain its reproductive capacity in shallow oceanic environments that commonly exhibit high UVR levels. Nevertheless, as a result of the inappropriate location of the artificial intercommunication structure of the Juluapan Lagoon with respect to the arrecifal area of study and therefore of the tides influence, other variables, such as the changes in short-term in turbidity, sediment inputs, nutrients, temperature and osmolarity, can act in combination and cause irreversible damage. The implementation of a management plan for the coralline reefs of the Mexican Pacific coast is required. Rev. Biol. Trop. 58 (1): 103-118. Epub 2010 March 01.<br>Los arrecifes de coral se ven afectados por una serie de variables ambientales que afectan su crecimiento y supervivencia, siendo los principales factores la alta irradiación y las fluctuaciones de temperatura. Los especímenes de Pocillopora capitata Verrill 1864 fueron expuestos a radiación activa fotosintéticamente (PAR) y radiación ultravioleta (RUV) por 32h en condiciones de laboratorio. Nosotros determinamos las concentraciones de peroxidación lipídica (MDA), actividades de enzimas antioxidantes (SOD, CAT, GPx y GST), clorofila a (Chl a), pigmentos carotenoides (CPS), aminoácidos tipo micosporina (MAAS), y la expulsión de las zooxantelas. Nuestros resultados muestran que los corales expuestos a los rayos UV presentaban niveles relativamente bajos de carotenoides y actividad de las enzimas antioxidantes en comparación con los expuestos al PAR, así como tasas de CPs/Chl a bajas. Aunque MAAs y CPs se producen rápidamente como antioxidantes no enzimáticos en respuesta a la radiación ultravioleta en los corales, éstos no fueron suficientes, incluso en la fase oscura del experimento, para mitigar los daños causados por la formación de especies reactivas de oxígeno (ROS), lo que provocó una ruptura en la relación simbiótica entre las zooxantelas y el coral con una relación 33 veces mayor que en el tratamiento de PAR. A nivel enzimático, P capitata presentó una serie de ajustes orientados a resistir los efectos derivados de la propagación de ROS y con ello favorecer su adaptación y capacidad reproductiva en ambientes oceánicos caracterizados por altos niveles de UVR.http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442010000100008antioxidantescoralestrés oxidativoMAAsperoxidación lipidicaPocillopora capitataradiación activa fotosintéticaSODCATGPxGSTchlorofila aespecies reactivas de oxígenoantioxidantscarotenoidscoralsoxidative stressMAAsUVRlipid peroxidationPocillopora capitataphotosynthetically active radiationSODCATGPxGSTchlorophyll areactive oxygen species |
| spellingShingle | Marco A Liñán-Cabello Laura A Flores-Ramírez José Francisco Cobo-Díaz Tania Zenteno- Savin Norma O Olguín-Monroy Aramís Olivos-Ortiz Adrián Tintos-Gómez Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) Revista de Biología Tropical antioxidantes coral estrés oxidativo MAAs peroxidación lipidica Pocillopora capitata radiación activa fotosintética SOD CAT GPx GST chlorofila a especies reactivas de oxígeno antioxidants carotenoids corals oxidative stress MAAs UVR lipid peroxidation Pocillopora capitata photosynthetically active radiation SOD CAT GPx GST chlorophyll a reactive oxygen species |
| title | Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) |
| title_full | Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) |
| title_fullStr | Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) |
| title_full_unstemmed | Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) |
| title_short | Response to short term ultraviolet stress in the reef-building coral Pocillopora capitata (Anthozoa: Scleractinia) |
| title_sort | response to short term ultraviolet stress in the reef building coral pocillopora capitata anthozoa scleractinia |
| topic | antioxidantes coral estrés oxidativo MAAs peroxidación lipidica Pocillopora capitata radiación activa fotosintética SOD CAT GPx GST chlorofila a especies reactivas de oxígeno antioxidants carotenoids corals oxidative stress MAAs UVR lipid peroxidation Pocillopora capitata photosynthetically active radiation SOD CAT GPx GST chlorophyll a reactive oxygen species |
| url | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442010000100008 |
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