Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection
The improper use of pesticides and excessive doses in the long term contribute to climate change and even threaten human health, organisms, and the balance of the ecosystem. A pesticide detection device is needed to monitor its levels to minimize risks to human health and the environment. A paper bi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/104/e3sconf_9th-iccc_01026.pdf |
| _version_ | 1827373098675994624 |
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| author | Hermanto Dhony Ismillayli Nurul Hamdiani Saprini Kamali Siti Raudhatul Wirawan Rahadi Muliasari Handa |
| author_facet | Hermanto Dhony Ismillayli Nurul Hamdiani Saprini Kamali Siti Raudhatul Wirawan Rahadi Muliasari Handa |
| author_sort | Hermanto Dhony |
| collection | DOAJ |
| description | The improper use of pesticides and excessive doses in the long term contribute to climate change and even threaten human health, organisms, and the balance of the ecosystem. A pesticide detection device is needed to monitor its levels to minimize risks to human health and the environment. A paper biosensor was developed in this study to detect organophosphates by utilizing the enzyme acetylcholine esterase (AChE) and silver nanoparticles (AgNP). The Whatman filter paper was used as a visual OP detection zone. AgNP, as an indicator, is adsorbed and gives a brownish-yellow color to the paper, while AChE is immobilized into the film and layered on the paper. The addition of acetylthiocholine chloride as a substrate to the film released thiocholine products which could replace the capping AgNPs causing the AgNPs to aggregate and the paper color to pale. The presence of OP in the sample will inhibit AChE activity so that paper fading is reduced. The biosensor response is quantized as an RGB value, which is determined using an application on a smartphone. The resulting biosensor has excellent performance with a linear range of 0.05-2.00 mg/L, a detection limit of 0.04 mg/L, and a CV of 0.48%. Biosensor measurements on vegetable samples showed conformity with the GCMS results as the standard method. Therefore, this biosensor is suitable for the on-site detection of pesticides offering easy, fast, and inexpensive analysis. |
| first_indexed | 2024-03-08T11:11:51Z |
| format | Article |
| id | doaj.art-e1ead578a87945f5b543390a6924becd |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-08T11:11:51Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-e1ead578a87945f5b543390a6924becd2024-01-26T10:44:01ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014670102610.1051/e3sconf/202346701026e3sconf_9th-iccc_01026Paper biosensors utilize silver nanoparticles for onsite pesticide residue detectionHermanto Dhony0Ismillayli Nurul1Hamdiani Saprini2Kamali Siti Raudhatul3Wirawan Rahadi4Muliasari Handa5Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of MataramDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of MataramDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of MataramDepartment of Environmental Science, Faculty of Mathematics and Natural Sciences, University of MataramDepartment of Physics, Faculty of Mathematics and Natural Sciences, University of MataramDepartment of Pharmacy, Faculty of Health, University of MataramThe improper use of pesticides and excessive doses in the long term contribute to climate change and even threaten human health, organisms, and the balance of the ecosystem. A pesticide detection device is needed to monitor its levels to minimize risks to human health and the environment. A paper biosensor was developed in this study to detect organophosphates by utilizing the enzyme acetylcholine esterase (AChE) and silver nanoparticles (AgNP). The Whatman filter paper was used as a visual OP detection zone. AgNP, as an indicator, is adsorbed and gives a brownish-yellow color to the paper, while AChE is immobilized into the film and layered on the paper. The addition of acetylthiocholine chloride as a substrate to the film released thiocholine products which could replace the capping AgNPs causing the AgNPs to aggregate and the paper color to pale. The presence of OP in the sample will inhibit AChE activity so that paper fading is reduced. The biosensor response is quantized as an RGB value, which is determined using an application on a smartphone. The resulting biosensor has excellent performance with a linear range of 0.05-2.00 mg/L, a detection limit of 0.04 mg/L, and a CV of 0.48%. Biosensor measurements on vegetable samples showed conformity with the GCMS results as the standard method. Therefore, this biosensor is suitable for the on-site detection of pesticides offering easy, fast, and inexpensive analysis.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/104/e3sconf_9th-iccc_01026.pdf |
| spellingShingle | Hermanto Dhony Ismillayli Nurul Hamdiani Saprini Kamali Siti Raudhatul Wirawan Rahadi Muliasari Handa Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection E3S Web of Conferences |
| title | Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| title_full | Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| title_fullStr | Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| title_full_unstemmed | Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| title_short | Paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| title_sort | paper biosensors utilize silver nanoparticles for onsite pesticide residue detection |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/104/e3sconf_9th-iccc_01026.pdf |
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