Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation.
UNLABELLED: Since gastrointestinal mucosa is constantly exposed to reactive oxygen species from various sources, the presence of antioxidants may contribute to the body's natural defenses against inflammatory diseases. HYPOTHESIS: To define the polyphenols extracted from dried apple peels (DAPP...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3553108?pdf=render |
| _version_ | 1818033017702055936 |
|---|---|
| author | Marie Claude Denis Alexandra Furtos Stéphanie Dudonné Alain Montoudis Carole Garofalo Yves Desjardins Edgard Delvin Emile Levy |
| author_facet | Marie Claude Denis Alexandra Furtos Stéphanie Dudonné Alain Montoudis Carole Garofalo Yves Desjardins Edgard Delvin Emile Levy |
| author_sort | Marie Claude Denis |
| collection | DOAJ |
| description | UNLABELLED: Since gastrointestinal mucosa is constantly exposed to reactive oxygen species from various sources, the presence of antioxidants may contribute to the body's natural defenses against inflammatory diseases. HYPOTHESIS: To define the polyphenols extracted from dried apple peels (DAPP) and determine their antioxidant and anti-inflammatory potential in the intestine. Caco-2/15 cells were used to study the role of DAPP preventive actions against oxidative stress (OxS) and inflammation induced by iron-ascorbate (Fe/Asc) and lipopolysaccharide (LPS), respectively. RESULTS: The combination of HPLC with fluorescence detection, HPLC-ESI-MS TOF and UPLC-ESI-MS/MS QQQ allowed us to characterize the phenolic compounds present in the DAPP (phenolic acids, flavonol glycosides, flavan-3-ols, procyanidins). The addition of Fe/Asc to Caco-2/15 cells induced OxS as demonstrated by the rise in malondialdehyde, depletion of n-3 polyunsaturated fatty acids, and alterations in the activity of endogenous antioxidants (SOD, GPx, G-Red). However, preincubation with DAPP prevented Fe/Asc-mediated lipid peroxidation and counteracted LPS-mediated inflammation as evidenced by the down-regulation of cytokines (TNF-α and IL-6), and prostaglandin E2. The mechanisms of action triggered by DAPP induced also a down-regulation of cyclooxygenase-2 and nuclear factor-κB, respectively. These actions were accompanied by the induction of Nrf2 (orchestrating cellular antioxidant defenses and maintaining redox homeostasis), and PGC-1α (the "master controller" of mitochondrial biogenesis). CONCLUSION: Our findings provide evidence of the capacity of DAPP to reduce OxS and inflammation, two pivotal processes involved in inflammatory bowel diseases. |
| first_indexed | 2024-12-10T06:16:35Z |
| format | Article |
| id | doaj.art-e1ef536a7f2f4239987d61f89781a4c3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T06:16:35Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-e1ef536a7f2f4239987d61f89781a4c32022-12-22T01:59:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5372510.1371/journal.pone.0053725Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation.Marie Claude DenisAlexandra FurtosStéphanie DudonnéAlain MontoudisCarole GarofaloYves DesjardinsEdgard DelvinEmile LevyUNLABELLED: Since gastrointestinal mucosa is constantly exposed to reactive oxygen species from various sources, the presence of antioxidants may contribute to the body's natural defenses against inflammatory diseases. HYPOTHESIS: To define the polyphenols extracted from dried apple peels (DAPP) and determine their antioxidant and anti-inflammatory potential in the intestine. Caco-2/15 cells were used to study the role of DAPP preventive actions against oxidative stress (OxS) and inflammation induced by iron-ascorbate (Fe/Asc) and lipopolysaccharide (LPS), respectively. RESULTS: The combination of HPLC with fluorescence detection, HPLC-ESI-MS TOF and UPLC-ESI-MS/MS QQQ allowed us to characterize the phenolic compounds present in the DAPP (phenolic acids, flavonol glycosides, flavan-3-ols, procyanidins). The addition of Fe/Asc to Caco-2/15 cells induced OxS as demonstrated by the rise in malondialdehyde, depletion of n-3 polyunsaturated fatty acids, and alterations in the activity of endogenous antioxidants (SOD, GPx, G-Red). However, preincubation with DAPP prevented Fe/Asc-mediated lipid peroxidation and counteracted LPS-mediated inflammation as evidenced by the down-regulation of cytokines (TNF-α and IL-6), and prostaglandin E2. The mechanisms of action triggered by DAPP induced also a down-regulation of cyclooxygenase-2 and nuclear factor-κB, respectively. These actions were accompanied by the induction of Nrf2 (orchestrating cellular antioxidant defenses and maintaining redox homeostasis), and PGC-1α (the "master controller" of mitochondrial biogenesis). CONCLUSION: Our findings provide evidence of the capacity of DAPP to reduce OxS and inflammation, two pivotal processes involved in inflammatory bowel diseases.http://europepmc.org/articles/PMC3553108?pdf=render |
| spellingShingle | Marie Claude Denis Alexandra Furtos Stéphanie Dudonné Alain Montoudis Carole Garofalo Yves Desjardins Edgard Delvin Emile Levy Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. PLoS ONE |
| title | Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. |
| title_full | Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. |
| title_fullStr | Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. |
| title_full_unstemmed | Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. |
| title_short | Apple peel polyphenols and their beneficial actions on oxidative stress and inflammation. |
| title_sort | apple peel polyphenols and their beneficial actions on oxidative stress and inflammation |
| url | http://europepmc.org/articles/PMC3553108?pdf=render |
| work_keys_str_mv | AT marieclaudedenis applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT alexandrafurtos applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT stephaniedudonne applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT alainmontoudis applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT carolegarofalo applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT yvesdesjardins applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT edgarddelvin applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation AT emilelevy applepeelpolyphenolsandtheirbeneficialactionsonoxidativestressandinflammation |