Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP
<p><strong>Background</strong>: Leishmaniasis is a major medical health problem and distributes in nearly half of 31 provinces of Iran. We aimed to identify cutaneous and visceral <em>Leishmania</em> spp. isolated from infected humans and domestic dogs in various region...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-06-01
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| Series: | Journal of Arthropod-Borne Diseases |
| Subjects: | |
| Online Access: | http://jad.tums.ac.ir/index.php/jad/article/view/890 |
| _version_ | 1819177350445662208 |
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| author | Aref Teimouri Mehdi Mohebali Elham Kazemirad Homa Hajjaran |
| author_facet | Aref Teimouri Mehdi Mohebali Elham Kazemirad Homa Hajjaran |
| author_sort | Aref Teimouri |
| collection | DOAJ |
| description | <p><strong>Background</strong>: Leishmaniasis is a major medical health problem and distributes in nearly half of 31 provinces of Iran. We aimed to identify cutaneous and visceral <em>Leishmania</em> spp. isolated from infected humans and domestic dogs in various regions of Iran, 2010‒2013.<strong><br />Methods: </strong>DNA was extracted from 108 lesion exudate samples of suspected patients to cutaneous leishmaniasis and nine liver and spleen aspirates of infected dogs cultured in RPMI-1640 and amplified using partial sequence of ITS1 gene. The PCR amplicons were digested using <em>Hae</em>III endonuclease enzyme and used in restriction fragment length polymorphism (RFLP) assay. Then, 48 amplicons representing various hosts were sequenced and compared to sequences from GenBank databases using BLAST.<strong><br />Results:</strong> PCR-RFLP analysis showed that 60 and 48 CL patients were infected by <em>Leishmania tropica</em> and <em>L. major</em>, respectively. From nine canine visceral leishmaniasis (CVL) isolates, eight isolates were identified as <em>L. infantum</em> and one as <em>L. tropica</em>. The greatest similarity of 95.7% in ITS1 region was seen between <em>L. infantum</em> and <em>L. major</em>. Furthermore, the lowest similarity with 65.7% was seen between <em>L. tropica</em> and <em>L. major</em>. Intra-species comparison of ITS1 region in <em>L. infantum, L. major </em>and<em> L. tropica</em> isolates were showed 100%, 98.2% and 72.4 % similarities, respectively.<strong><br />Conclusion:</strong> PCR-RFLP based on ITS1 region is an appropriate method to distinguish three <em>Leishmania</em> spp. of <em>L. major</em>, <em>L. tropica,</em> and <em>L. infantum</em>. In intra-species comparison of ITS1 region, genotypic variations showed that <em>L. tropica</em> isolates were more heterogeneous than <em>L. major</em> and <em>L. infantum</em> isolates.</p> |
| first_indexed | 2024-12-22T21:25:16Z |
| format | Article |
| id | doaj.art-e1efe36921454c1eb84d02dc1da479e0 |
| institution | Directory Open Access Journal |
| issn | 1735-7179 2322-2271 |
| language | English |
| last_indexed | 2024-12-22T21:25:16Z |
| publishDate | 2018-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj.art-e1efe36921454c1eb84d02dc1da479e02022-12-21T18:12:04ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases1735-71792322-22712018-06-01121385Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLPAref Teimouri0Mehdi Mohebali1Elham Kazemirad2Homa Hajjaran3Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran Students Scientific Research Center, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences,Tehran, IranDepartment of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran<p><strong>Background</strong>: Leishmaniasis is a major medical health problem and distributes in nearly half of 31 provinces of Iran. We aimed to identify cutaneous and visceral <em>Leishmania</em> spp. isolated from infected humans and domestic dogs in various regions of Iran, 2010‒2013.<strong><br />Methods: </strong>DNA was extracted from 108 lesion exudate samples of suspected patients to cutaneous leishmaniasis and nine liver and spleen aspirates of infected dogs cultured in RPMI-1640 and amplified using partial sequence of ITS1 gene. The PCR amplicons were digested using <em>Hae</em>III endonuclease enzyme and used in restriction fragment length polymorphism (RFLP) assay. Then, 48 amplicons representing various hosts were sequenced and compared to sequences from GenBank databases using BLAST.<strong><br />Results:</strong> PCR-RFLP analysis showed that 60 and 48 CL patients were infected by <em>Leishmania tropica</em> and <em>L. major</em>, respectively. From nine canine visceral leishmaniasis (CVL) isolates, eight isolates were identified as <em>L. infantum</em> and one as <em>L. tropica</em>. The greatest similarity of 95.7% in ITS1 region was seen between <em>L. infantum</em> and <em>L. major</em>. Furthermore, the lowest similarity with 65.7% was seen between <em>L. tropica</em> and <em>L. major</em>. Intra-species comparison of ITS1 region in <em>L. infantum, L. major </em>and<em> L. tropica</em> isolates were showed 100%, 98.2% and 72.4 % similarities, respectively.<strong><br />Conclusion:</strong> PCR-RFLP based on ITS1 region is an appropriate method to distinguish three <em>Leishmania</em> spp. of <em>L. major</em>, <em>L. tropica,</em> and <em>L. infantum</em>. In intra-species comparison of ITS1 region, genotypic variations showed that <em>L. tropica</em> isolates were more heterogeneous than <em>L. major</em> and <em>L. infantum</em> isolates.</p>http://jad.tums.ac.ir/index.php/jad/article/view/890Leishmania, ITS1 gene, PCR- RFLP, Iran |
| spellingShingle | Aref Teimouri Mehdi Mohebali Elham Kazemirad Homa Hajjaran Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP Journal of Arthropod-Borne Diseases Leishmania, ITS1 gene, PCR- RFLP, Iran |
| title | Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP |
| title_full | Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP |
| title_fullStr | Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP |
| title_full_unstemmed | Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP |
| title_short | Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP |
| title_sort | molecular identification of agents of human cutaneous leishmaniasis and canine visceral leishmaniasis in different areas of iran using internal transcribed spacer 1 pcr rflp |
| topic | Leishmania, ITS1 gene, PCR- RFLP, Iran |
| url | http://jad.tums.ac.ir/index.php/jad/article/view/890 |
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