Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin
Background: Antifungal susceptibility testing remains an area of intense interest because of the increasing number of clinical isolates resistant to antifungal therapy. Clinical and Laboratory Standards Institute has proposed reference broth micro dilution (BMD) method for susceptibility testing...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/6735/14119_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
| _version_ | 1818454924851150848 |
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| author | Deepak Kumar Sayan Bhattacharyya Prashant Gupta Gopa Banerjee Mastan Singh |
| author_facet | Deepak Kumar Sayan Bhattacharyya Prashant Gupta Gopa Banerjee Mastan Singh |
| author_sort | Deepak Kumar |
| collection | DOAJ |
| description | Background: Antifungal susceptibility testing remains an area
of intense interest because of the increasing number of clinical
isolates resistant to antifungal therapy. Clinical and Laboratory
Standards Institute has proposed reference broth micro dilution
(BMD) method for susceptibility testing. The reference method is
time-consuming and poorly suited for the routine clinical laboratory
setting. Agar-based susceptibility testing methods, disk diffusion
(DD) method and the E-test method can be an easier, reliable and
less time consuming alternative for the BMD method.
Aim: To compare the results of Amphotericin B, fluconazole,
voriconazole, and Caspofungin susceptibility testing by DD, and
the E-test method with the CLSI reference method for clinical
Candida isolates.
Materials and Methods: Broth Microdilution (BMD), E-test
and Disk diffusion testing of the various clinical Candida isolates
was performed in accordance with CLSI documents. The results
obtained were analysed and compared.
Results: The categorical agreement for Amphotericin B, fluconazole,
voriconazole, and Caspofungin susceptibility results by E-test and
DD method was 65.2%, 67.4%; 100%, 82.6%; 100%, 100%;
100%, 97.8% respectively.
Conclusion: The agar-based E-test and disk diffusion methods are
reliable alternatives to the BMD method for Candida isolates when
test susceptible to fluconazole, voriconazole, and Caspofungin,
however the susceptibility testing results must be interpreted with
caution in case of Amphotericin B. |
| first_indexed | 2024-12-14T22:02:37Z |
| format | Article |
| id | doaj.art-e1f0d726228149ca8230749ad761fba0 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T22:02:37Z |
| publishDate | 2015-11-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-e1f0d726228149ca8230749ad761fba02022-12-21T22:45:56ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-11-01911DC01DC0410.7860/JCDR/2015/14119.6735Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and CaspofunginDeepak Kumar0Sayan Bhattacharyya1Prashant Gupta2Gopa Banerjee3Mastan Singh4Assistant Professor, Department of Microbiology, IMS, BHU Varanasi, U.P., India.Assistant Professor, Department of Microbiology, AIIMS, Patna, India. Assistant Professor, Department of Microbiology, King George’s Medical University, Lucknow, India. Professor, Department of Microbiology, King George’s Medical University, Lucknow, India. Professor and HOD, Department of Microbiology, King George’s Medical University, Lucknow, India.Background: Antifungal susceptibility testing remains an area of intense interest because of the increasing number of clinical isolates resistant to antifungal therapy. Clinical and Laboratory Standards Institute has proposed reference broth micro dilution (BMD) method for susceptibility testing. The reference method is time-consuming and poorly suited for the routine clinical laboratory setting. Agar-based susceptibility testing methods, disk diffusion (DD) method and the E-test method can be an easier, reliable and less time consuming alternative for the BMD method. Aim: To compare the results of Amphotericin B, fluconazole, voriconazole, and Caspofungin susceptibility testing by DD, and the E-test method with the CLSI reference method for clinical Candida isolates. Materials and Methods: Broth Microdilution (BMD), E-test and Disk diffusion testing of the various clinical Candida isolates was performed in accordance with CLSI documents. The results obtained were analysed and compared. Results: The categorical agreement for Amphotericin B, fluconazole, voriconazole, and Caspofungin susceptibility results by E-test and DD method was 65.2%, 67.4%; 100%, 82.6%; 100%, 100%; 100%, 97.8% respectively. Conclusion: The agar-based E-test and disk diffusion methods are reliable alternatives to the BMD method for Candida isolates when test susceptible to fluconazole, voriconazole, and Caspofungin, however the susceptibility testing results must be interpreted with caution in case of Amphotericin B.https://jcdr.net/articles/PDF/6735/14119_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdfantifungal susceptibility testingantifungal therapyclsi reference method |
| spellingShingle | Deepak Kumar Sayan Bhattacharyya Prashant Gupta Gopa Banerjee Mastan Singh Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin Journal of Clinical and Diagnostic Research antifungal susceptibility testing antifungal therapy clsi reference method |
| title | Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin |
| title_full | Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin |
| title_fullStr | Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin |
| title_full_unstemmed | Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin |
| title_short | Comparative Analysis of Disc Diffusion and E-test with Broth Micro-dilution for Susceptibility Testing of Clinical Candida Isolates Against Amphotericin B, Fluconazole, Voriconazole and Caspofungin |
| title_sort | comparative analysis of disc diffusion and e test with broth micro dilution for susceptibility testing of clinical candida isolates against amphotericin b fluconazole voriconazole and caspofungin |
| topic | antifungal susceptibility testing antifungal therapy clsi reference method |
| url | https://jcdr.net/articles/PDF/6735/14119_CE(RA1)_F(T)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf |
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