Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis
<b>Background :</b> Problems in the diagnosis of tuberculosis using smear and culture techniques have necessitated the exploration of the utility of serodiagnosis to support clinical suspicion of tuberculosis. Anti-A60 antigen IgG enzyme-linked immunosorbent assay (ELISA) was evaluated a...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2007-01-01
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| Series: | Annals of Thoracic Medicine |
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| Online Access: | http://www.thoracicmedicine.org/article.asp?issn=1817-1737;year=2007;volume=2;issue=2;spage=47;epage=51;aulast=Bukhary |
| _version_ | 1819072200495333376 |
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| author | Bukhary Zakeya |
| author_facet | Bukhary Zakeya |
| author_sort | Bukhary Zakeya |
| collection | DOAJ |
| description | <b>Background :</b> Problems in the diagnosis of tuberculosis using smear and culture techniques have necessitated the exploration of the utility of serodiagnosis to support clinical suspicion of tuberculosis. Anti-A60 antigen IgG enzyme-linked immunosorbent assay (ELISA) was evaluated as a tool for the diagnosis of active pulmonary tuberculosis. <b>Materials and Methods : </b> ELISA was used for the detection of immunoglobulin G (IgG) against A60 antigen in parallel with other familiar diagnostic methods in 70 patients with pulmonary tuberculosis (Group_I) along with 70 controls showing evidence of latent tuberculosis infection (Group II). <b> Results:</b> ELISA results were positive in 63 (90%) patients in Group_I compared to three (4%) controls in Group_II. Group_I patients had significantly higher titers of IgG (mean = 750.79 ± 115.87 U/ml against the A60 antigen as compared to Group II controls (mean = 206.67 ± 20.81 U/ml (<i> P </i> < 0.0001). The overall sensitivity and specificity obtained using ELISA were 90 and 95.7% respectively in active pulmonary tuberculosis. Ziehl-Nielsen (Z-N) stains for acid-fast bacilli were positive in 60 (85.7%) patients. In 48 (68.6%) patients, <i> M. tuberculosis</i> grew on both Lowenstein-Jensen (L-J) medium and BACTEC MGIT 960 liquid medium with mean detection times of 45 and 8 days respectively. Tuberculin skin test was positive in 38 (54.3%) patients. Chest X-ray results were consistent with the diagnosis of pulmonary tuberculosis in 53 (75.7%) patients. <b> Conclusion :</b> Anti-A60 IgG ELISA results were significantly positive and associated with elevated antibody titers in pulmonary tuberculosis as compared to latent mycobacterium<i> </i> infection. The high diagnostic performance of the test makes it a useful, simple and rapid supporting tool to validate clinical suspicion of active<i> </i> pulmonary disease. |
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| format | Article |
| id | doaj.art-e1f3159828af422cab66b202abd81eb8 |
| institution | Directory Open Access Journal |
| issn | 1817-1737 |
| language | English |
| last_indexed | 2024-12-21T17:33:57Z |
| publishDate | 2007-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Annals of Thoracic Medicine |
| spelling | doaj.art-e1f3159828af422cab66b202abd81eb82022-12-21T18:55:50ZengWolters Kluwer Medknow PublicationsAnnals of Thoracic Medicine1817-17372007-01-01224751Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosisBukhary Zakeya<b>Background :</b> Problems in the diagnosis of tuberculosis using smear and culture techniques have necessitated the exploration of the utility of serodiagnosis to support clinical suspicion of tuberculosis. Anti-A60 antigen IgG enzyme-linked immunosorbent assay (ELISA) was evaluated as a tool for the diagnosis of active pulmonary tuberculosis. <b>Materials and Methods : </b> ELISA was used for the detection of immunoglobulin G (IgG) against A60 antigen in parallel with other familiar diagnostic methods in 70 patients with pulmonary tuberculosis (Group_I) along with 70 controls showing evidence of latent tuberculosis infection (Group II). <b> Results:</b> ELISA results were positive in 63 (90%) patients in Group_I compared to three (4%) controls in Group_II. Group_I patients had significantly higher titers of IgG (mean = 750.79 ± 115.87 U/ml against the A60 antigen as compared to Group II controls (mean = 206.67 ± 20.81 U/ml (<i> P </i> < 0.0001). The overall sensitivity and specificity obtained using ELISA were 90 and 95.7% respectively in active pulmonary tuberculosis. Ziehl-Nielsen (Z-N) stains for acid-fast bacilli were positive in 60 (85.7%) patients. In 48 (68.6%) patients, <i> M. tuberculosis</i> grew on both Lowenstein-Jensen (L-J) medium and BACTEC MGIT 960 liquid medium with mean detection times of 45 and 8 days respectively. Tuberculin skin test was positive in 38 (54.3%) patients. Chest X-ray results were consistent with the diagnosis of pulmonary tuberculosis in 53 (75.7%) patients. <b> Conclusion :</b> Anti-A60 IgG ELISA results were significantly positive and associated with elevated antibody titers in pulmonary tuberculosis as compared to latent mycobacterium<i> </i> infection. The high diagnostic performance of the test makes it a useful, simple and rapid supporting tool to validate clinical suspicion of active<i> </i> pulmonary disease.http://www.thoracicmedicine.org/article.asp?issn=1817-1737;year=2007;volume=2;issue=2;spage=47;epage=51;aulast=BukharyA60 antigenenzyme-linked immunosorbent assaypulmonary tuberculosisserodiagnosis |
| spellingShingle | Bukhary Zakeya Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis Annals of Thoracic Medicine A60 antigen enzyme-linked immunosorbent assay pulmonary tuberculosis serodiagnosis |
| title | Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| title_full | Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| title_fullStr | Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| title_full_unstemmed | Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| title_short | Evaluation of anti-A60 antigen IgG enzyme-linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| title_sort | evaluation of anti a60 antigen igg enzyme linked immunosorbent assay for serodiagnosis of pulmonary tuberculosis |
| topic | A60 antigen enzyme-linked immunosorbent assay pulmonary tuberculosis serodiagnosis |
| url | http://www.thoracicmedicine.org/article.asp?issn=1817-1737;year=2007;volume=2;issue=2;spage=47;epage=51;aulast=Bukhary |
| work_keys_str_mv | AT bukharyzakeya evaluationofantia60antigeniggenzymelinkedimmunosorbentassayforserodiagnosisofpulmonarytuberculosis |