Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc.
Flavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1025317/full |
| _version_ | 1811198477255835648 |
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| author | Yubo Wang Minghui Zhang Kuiling Dong Xiaojuan Yin Chunhui Hao Wenge Zhang Muhammad Irfan Lijing Chen Yong Wang |
| author_facet | Yubo Wang Minghui Zhang Kuiling Dong Xiaojuan Yin Chunhui Hao Wenge Zhang Muhammad Irfan Lijing Chen Yong Wang |
| author_sort | Yubo Wang |
| collection | DOAJ |
| description | Flavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in the fruit ripening period (August to September) of two main cultivated varieties in northern China. The ethanol extract was prepared, and the potential flavonoids of Chrysin (Flavone1), Rutin (Flavone2), and Daidzein (Flavone3) in Actinidia arguta Sieb. Zucc. were separated and purified by HPD600 macroporous adsorption resin and preparative liquid chromatography. The structure was identified by MS-HPLC, and the serum uric acid index of male Kunming mice was determined by an animal model test.125 flavonoids and 50 differentially regulated genes were identified. The contents of UA (uric acid), BUN (urea nitrogen), Cr (creatinine), and GAPDH in mouse serum and mouse liver glycogen decreased or increased in varying degrees. This paper reveals the biosynthetic pathway of uric acid-reducing flavonoids in the fruit of Actinidia arguta Sieb. Zucc., a major cultivar in northern China, provides valuable information for the development of food and drug homologous functional foods. |
| first_indexed | 2024-04-12T01:32:29Z |
| format | Article |
| id | doaj.art-e1f83bb6c31f42e9af98ef183e746798 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T01:32:29Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e1f83bb6c31f42e9af98ef183e7467982022-12-22T03:53:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10253171025317Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc.Yubo Wang0Minghui Zhang1Kuiling Dong2Xiaojuan Yin3Chunhui Hao4Wenge Zhang5Muhammad Irfan6Lijing Chen7Yong Wang8Key Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaOriental Language Institute, Mudanjiang Normal University, Mudanjiang, ChinaKey Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaPet Medicine Teaching and Research Office, Liaoning Agricultural College, Yingkou, ChinaBiochemistry Teaching and Research Office, Anshan Health School, Anshan, ChinaDepartment of Biotechnology, University of Sargodha, Sargodha, PakistanKey Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Chemical Engineering, Liaoning University of Science and Technology, Anshan, ChinaFlavonoids from Actinidia arguta Sieb. Zucc. can reduce uric acid in mice. However, the molecular basis of its biosynthesis is still unclear. In this paper, we used a combination of extensively targeted metabolomics and transcriptomics analysis to determine the types and differences of flavonoids in the fruit ripening period (August to September) of two main cultivated varieties in northern China. The ethanol extract was prepared, and the potential flavonoids of Chrysin (Flavone1), Rutin (Flavone2), and Daidzein (Flavone3) in Actinidia arguta Sieb. Zucc. were separated and purified by HPD600 macroporous adsorption resin and preparative liquid chromatography. The structure was identified by MS-HPLC, and the serum uric acid index of male Kunming mice was determined by an animal model test.125 flavonoids and 50 differentially regulated genes were identified. The contents of UA (uric acid), BUN (urea nitrogen), Cr (creatinine), and GAPDH in mouse serum and mouse liver glycogen decreased or increased in varying degrees. This paper reveals the biosynthetic pathway of uric acid-reducing flavonoids in the fruit of Actinidia arguta Sieb. Zucc., a major cultivar in northern China, provides valuable information for the development of food and drug homologous functional foods.https://www.frontiersin.org/articles/10.3389/fpls.2022.1025317/fullflavonoidsActinidia arguta Sieb.Zucc.transcriptomewidely targeted metabonomicsbiosynthetic pathway |
| spellingShingle | Yubo Wang Minghui Zhang Kuiling Dong Xiaojuan Yin Chunhui Hao Wenge Zhang Muhammad Irfan Lijing Chen Yong Wang Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. Frontiers in Plant Science flavonoids Actinidia arguta Sieb.Zucc. transcriptome widely targeted metabonomics biosynthetic pathway |
| title | Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. |
| title_full | Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. |
| title_fullStr | Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. |
| title_full_unstemmed | Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. |
| title_short | Metabolomic and transcriptomic exploration of the uric acid-reducing flavonoids biosynthetic pathways in the fruit of Actinidia arguta Sieb. Zucc. |
| title_sort | metabolomic and transcriptomic exploration of the uric acid reducing flavonoids biosynthetic pathways in the fruit of actinidia arguta sieb zucc |
| topic | flavonoids Actinidia arguta Sieb.Zucc. transcriptome widely targeted metabonomics biosynthetic pathway |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1025317/full |
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