Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1
An investigation was made using a styrene-divinylbenzene copolymer as a solid phase sorbent to recover gangliosides from aqueous solutions. A comparison with octadecyl-bonded (C18) silica gel showed that the general procedure used to purify gangliosides on C18 silica gel could be used with the copol...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2002-08-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520300663 |
| _version_ | 1819072378146127872 |
|---|---|
| author | Iuliana Popa Cristina Vlad Jacques Bodennec Jacques Portoukalian |
| author_facet | Iuliana Popa Cristina Vlad Jacques Bodennec Jacques Portoukalian |
| author_sort | Iuliana Popa |
| collection | DOAJ |
| description | An investigation was made using a styrene-divinylbenzene copolymer as a solid phase sorbent to recover gangliosides from aqueous solutions. A comparison with octadecyl-bonded (C18) silica gel showed that the general procedure used to purify gangliosides on C18 silica gel could be used with the copolymer. The yield of gangliosides depended on various parameters such as the composition of the conditioning solution, the salt concentration of the loading solution, and the amount of applied gangliosides per gram of copolymer. In optimal conditions, the recovery of gangliosides and other lipids present in the upper phases of partition was higher than 95%. Using radiolabeled gangliosides, it was found that gangliosides present in serum-containing medium could also be quantitatively recovered on copolymer, provided the medium was diluted with an equal volume of methanol prior to its application onto the column.The major advantage of the copolymer is its high stability in acidic or alkaline conditions that allows multiple cycles of cleaning and reconditioning of the sorbent without alteration of its chromatographic properties. |
| first_indexed | 2024-12-21T17:36:46Z |
| format | Article |
| id | doaj.art-e1f9753df2254a0db653692068ce8cd6 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T17:36:46Z |
| publishDate | 2002-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-e1f9753df2254a0db653692068ce8cd62022-12-21T18:55:45ZengElsevierJournal of Lipid Research0022-22752002-08-0143813351340Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1Iuliana Popa0Cristina Vlad1Jacques Bodennec2Jacques Portoukalian3Laboratory of Dermatology, INSERM U.346, Hospital Edouard Herriot 69437 Lyon Cx 03, France; Institute of Macromolecular Chemistry Petru Poni, Aleea Gr. Ghica Voda 41A, Iassy 6600, RomaniaInstitute of Macromolecular Chemistry Petru Poni, Aleea Gr. Ghica Voda 41A, Iassy 6600, RomaniaLaboratory of Dermatology, INSERM U.346, Hospital Edouard Herriot 69437 Lyon Cx 03, FranceTo whom correspondence should be addressed.; Laboratory of Dermatology, INSERM U.346, Hospital Edouard Herriot 69437 Lyon Cx 03, FranceAn investigation was made using a styrene-divinylbenzene copolymer as a solid phase sorbent to recover gangliosides from aqueous solutions. A comparison with octadecyl-bonded (C18) silica gel showed that the general procedure used to purify gangliosides on C18 silica gel could be used with the copolymer. The yield of gangliosides depended on various parameters such as the composition of the conditioning solution, the salt concentration of the loading solution, and the amount of applied gangliosides per gram of copolymer. In optimal conditions, the recovery of gangliosides and other lipids present in the upper phases of partition was higher than 95%. Using radiolabeled gangliosides, it was found that gangliosides present in serum-containing medium could also be quantitatively recovered on copolymer, provided the medium was diluted with an equal volume of methanol prior to its application onto the column.The major advantage of the copolymer is its high stability in acidic or alkaline conditions that allows multiple cycles of cleaning and reconditioning of the sorbent without alteration of its chromatographic properties.http://www.sciencedirect.com/science/article/pii/S0022227520300663chromatographyisolationsolid phase extraction |
| spellingShingle | Iuliana Popa Cristina Vlad Jacques Bodennec Jacques Portoukalian Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 Journal of Lipid Research chromatography isolation solid phase extraction |
| title | Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 |
| title_full | Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 |
| title_fullStr | Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 |
| title_full_unstemmed | Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 |
| title_short | Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer columns1 |
| title_sort | recovery of gangliosides from aqueous solutions on styrene divinylbenzene copolymer columns1 |
| topic | chromatography isolation solid phase extraction |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520300663 |
| work_keys_str_mv | AT iulianapopa recoveryofgangliosidesfromaqueoussolutionsonstyrenedivinylbenzenecopolymercolumns1 AT cristinavlad recoveryofgangliosidesfromaqueoussolutionsonstyrenedivinylbenzenecopolymercolumns1 AT jacquesbodennec recoveryofgangliosidesfromaqueoussolutionsonstyrenedivinylbenzenecopolymercolumns1 AT jacquesportoukalian recoveryofgangliosidesfromaqueoussolutionsonstyrenedivinylbenzenecopolymercolumns1 |