Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens
Bacillus spp. are well known to protect plants from seed or soil-borne pathogens by the synthesis of various metabolites with antimicrobial activity, such as hydrolytic enzymes and antibiotics. This study aimed to select the most effective Bacillus spp. from a group of ten antagonistic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Matica srpska
2022-01-01
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| Series: | Zbornik Matice Srpske za Prirodne Nauke |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-4906/2022/0352-49062243065M.pdf |
| _version_ | 1827993709484965888 |
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| author | Miljaković Dragana Đ. Milošević Dragana N. Ignjatov Maja V. Marinković Jelena B. Tamindžić Gordana D. Tintor Branislava B. Nikolić Zorica T. |
| author_facet | Miljaković Dragana Đ. Milošević Dragana N. Ignjatov Maja V. Marinković Jelena B. Tamindžić Gordana D. Tintor Branislava B. Nikolić Zorica T. |
| author_sort | Miljaković Dragana Đ. |
| collection | DOAJ |
| description | Bacillus spp. are well known to protect plants from seed or soil-borne
pathogens by the synthesis of various metabolites with antimicrobial
activity, such as hydrolytic enzymes and antibiotics. This study aimed to
select the most effective Bacillus spp. from a group of ten antagonistic
strains by antifungal activity assay. Bacillus strains were primarily
isolated from the soil and identified as B. safensis, B. pumilus and B.
subtilis by 16S rDNA sequencing. The four analyzed fungi: Macrophomina
phaseolina, Alternaria alternata, Cladosporium cladosporoides, and
Sclerotinia sclerotiorum, were obtained from sunflower seeds and identified
using PCR analysis and primers specific for ITS region. The antifungal
activity of bacterial strains was examined in a dual plate assay. Bacillus
spp. demonstrated the highest antagonism against S. sclerotiorum, followed
by C. cladosporoides, M. phaseolina, and A. alternata, with an average
percentage of growth inhibition (PGI) of 77%, 70%, 64% and 59%,
respectively. Overall, Bacillus spp. included in this study demonstrated a
rather strong biocontrol potential, although the effect of particular strain
varied depending on the tested fungi. The highest antagonistic effect toward
M. phaseolina and A. alternata was exhibited by B. safensis B2 and B.
pumilus B3. B. pumilus B11 and B. subtilis B32 were the most efficient
against C. cladosporoides, whereas B. pumilus B3 and B. subtilis B7 had the
highest antifungal activity against S. sclerotiorum. Findings point to the
fact that the most effective Bacillus spp. could be used as potential
biocontrol agents for improving plant health and productivity. |
| first_indexed | 2024-04-10T04:30:01Z |
| format | Article |
| id | doaj.art-e200a57e5be449c196573ae4dce62398 |
| institution | Directory Open Access Journal |
| issn | 0352-4906 2406-0828 |
| language | English |
| last_indexed | 2024-04-10T04:30:01Z |
| publishDate | 2022-01-01 |
| publisher | Matica srpska |
| record_format | Article |
| series | Zbornik Matice Srpske za Prirodne Nauke |
| spelling | doaj.art-e200a57e5be449c196573ae4dce623982023-03-10T08:08:25ZengMatica srpskaZbornik Matice Srpske za Prirodne Nauke0352-49062406-08282022-01-012022143657210.2298/ZMSPN2243065M0352-49062243065MScreening of Bacillus spp. as potential biocontrol agents against sunflower pathogensMiljaković Dragana Đ.0Milošević Dragana N.1https://orcid.org/0000-0001-6392-9253Ignjatov Maja V.2https://orcid.org/0000-0003-4650-5082Marinković Jelena B.3https://orcid.org/0000-0003-3984-7930Tamindžić Gordana D.4https://orcid.org/0000-0002-6808-0129Tintor Branislava B.5https://orcid.org/0000-0002-2437-5064Nikolić Zorica T.6https://orcid.org/0000-0002-9333-6958Institute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaInstitute of Field and Vegetable Crops, Novi Sad, SerbiaBacillus spp. are well known to protect plants from seed or soil-borne pathogens by the synthesis of various metabolites with antimicrobial activity, such as hydrolytic enzymes and antibiotics. This study aimed to select the most effective Bacillus spp. from a group of ten antagonistic strains by antifungal activity assay. Bacillus strains were primarily isolated from the soil and identified as B. safensis, B. pumilus and B. subtilis by 16S rDNA sequencing. The four analyzed fungi: Macrophomina phaseolina, Alternaria alternata, Cladosporium cladosporoides, and Sclerotinia sclerotiorum, were obtained from sunflower seeds and identified using PCR analysis and primers specific for ITS region. The antifungal activity of bacterial strains was examined in a dual plate assay. Bacillus spp. demonstrated the highest antagonism against S. sclerotiorum, followed by C. cladosporoides, M. phaseolina, and A. alternata, with an average percentage of growth inhibition (PGI) of 77%, 70%, 64% and 59%, respectively. Overall, Bacillus spp. included in this study demonstrated a rather strong biocontrol potential, although the effect of particular strain varied depending on the tested fungi. The highest antagonistic effect toward M. phaseolina and A. alternata was exhibited by B. safensis B2 and B. pumilus B3. B. pumilus B11 and B. subtilis B32 were the most efficient against C. cladosporoides, whereas B. pumilus B3 and B. subtilis B7 had the highest antifungal activity against S. sclerotiorum. Findings point to the fact that the most effective Bacillus spp. could be used as potential biocontrol agents for improving plant health and productivity.http://www.doiserbia.nb.rs/img/doi/0352-4906/2022/0352-49062243065M.pdfalternariaantifungal activitybacillusbiocontrolcladosporiummacrophominasclerotiniasunflower |
| spellingShingle | Miljaković Dragana Đ. Milošević Dragana N. Ignjatov Maja V. Marinković Jelena B. Tamindžić Gordana D. Tintor Branislava B. Nikolić Zorica T. Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens Zbornik Matice Srpske za Prirodne Nauke alternaria antifungal activity bacillus biocontrol cladosporium macrophomina sclerotinia sunflower |
| title | Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens |
| title_full | Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens |
| title_fullStr | Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens |
| title_full_unstemmed | Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens |
| title_short | Screening of Bacillus spp. as potential biocontrol agents against sunflower pathogens |
| title_sort | screening of bacillus spp as potential biocontrol agents against sunflower pathogens |
| topic | alternaria antifungal activity bacillus biocontrol cladosporium macrophomina sclerotinia sunflower |
| url | http://www.doiserbia.nb.rs/img/doi/0352-4906/2022/0352-49062243065M.pdf |
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