The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia
Abstract Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-020-00173-5 |
| _version_ | 1830192607204999168 |
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| author | David W. Cleary Denise E. Morris Rebecca A. Anderson Jessica Jones Ahmed Ghazi Alattraqchi Nor Iza A. Rahman Salwani Ismail Mohd Sayuti Razali Rahmah Mohd Amin Aniza Abd Aziz Nor Kamaruzaman Esa Salman Amiruddin Ching Hoong Chew Hafis Simin Ramle Abdullah Chew Chieng Yeo Stuart C. Clarke |
| author_facet | David W. Cleary Denise E. Morris Rebecca A. Anderson Jessica Jones Ahmed Ghazi Alattraqchi Nor Iza A. Rahman Salwani Ismail Mohd Sayuti Razali Rahmah Mohd Amin Aniza Abd Aziz Nor Kamaruzaman Esa Salman Amiruddin Ching Hoong Chew Hafis Simin Ramle Abdullah Chew Chieng Yeo Stuart C. Clarke |
| author_sort | David W. Cleary |
| collection | DOAJ |
| description | Abstract Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous peoples of Malaysia. A total of 130 participants were recruited from two sites in the north-eastern state of Terengganu in Peninsular Malaysia. Using 16S rRNA sequencing, the nasal microbiome was significantly more diverse in those aged 5–17 years compared to 50+ years (p = 0.023) and clustered by age (PERMANOVA analysis of the Bray–Curtis distance, p = 0.001). Hierarchical clustering of Bray–Curtis dissimilarity scores revealed six microbiome clusters. The largest cluster (n = 28; 35.4%) had a marked abundance of Corynebacterium. In the oral microbiomes Streptococcus, Neisseria and Haemophilus were dominant. Using conventional microbiology, high levels of Staphylococcus aureus carriage were observed, particularly in the 18–65 age group (n = 17/36; 47.2% 95% CI: 30.9–63.5). The highest carriage of pneumococci was in the <5 and 5 to 17 year olds, with 57.1% (4/7) and 49.2% (30/61), respectively. Sixteen pneumococcal serotypes were identified, the most common being the nonvaccine-type 23A (14.6%) and the vaccine-type 6B (9.8%). The prevalence of pneumococcal serotypes covered by pneumococcal conjugate vaccines support introduction into a Malaysian national immunisation schedule. In addition, the dominance of Corynebacterium in the airway microbiomes is intriguing given their role as a potentially protective commensal with respect to acute infection and respiratory health. |
| first_indexed | 2024-12-17T23:59:21Z |
| format | Article |
| id | doaj.art-e20775c234fb4d2abe532842af2630a6 |
| institution | Directory Open Access Journal |
| issn | 2055-5008 |
| language | English |
| last_indexed | 2024-12-17T23:59:21Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj.art-e20775c234fb4d2abe532842af2630a62022-12-21T21:28:00ZengNature Portfolionpj Biofilms and Microbiomes2055-50082021-01-017111110.1038/s41522-020-00173-5The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular MalaysiaDavid W. Cleary0Denise E. Morris1Rebecca A. Anderson2Jessica Jones3Ahmed Ghazi Alattraqchi4Nor Iza A. Rahman5Salwani Ismail6Mohd Sayuti Razali7Rahmah Mohd Amin8Aniza Abd Aziz9Nor Kamaruzaman Esa10Salman Amiruddin11Ching Hoong Chew12Hafis Simin13Ramle Abdullah14Chew Chieng Yeo15Stuart C. Clarke16Faculty of Medicine and Institute for Life Sciences, University of SouthamptonFaculty of Medicine and Institute for Life Sciences, University of SouthamptonFaculty of Medicine and Institute for Life Sciences, University of SouthamptonFaculty of Medicine and Institute for Life Sciences, University of SouthamptonFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Health Sciences, Universiti Sultan Zainal Abidin, Gong Badak CampusFaculty of Applied Social Sciences, Universiti Sultan Zainal Abidin, Gong Badak CampusFaculty of Applied Social Sciences, Universiti Sultan Zainal Abidin, Gong Badak CampusFaculty of Medicine, Universiti Sultan Zainal Abidin, Medical CampusFaculty of Medicine and Institute for Life Sciences, University of SouthamptonAbstract Much microbiome research has focused on populations that are predominantly of European descent, and from narrow demographics that do not capture the socio-economic and lifestyle differences which impact human health. Here we examined the airway microbiomes of the Orang Asli, the indigenous peoples of Malaysia. A total of 130 participants were recruited from two sites in the north-eastern state of Terengganu in Peninsular Malaysia. Using 16S rRNA sequencing, the nasal microbiome was significantly more diverse in those aged 5–17 years compared to 50+ years (p = 0.023) and clustered by age (PERMANOVA analysis of the Bray–Curtis distance, p = 0.001). Hierarchical clustering of Bray–Curtis dissimilarity scores revealed six microbiome clusters. The largest cluster (n = 28; 35.4%) had a marked abundance of Corynebacterium. In the oral microbiomes Streptococcus, Neisseria and Haemophilus were dominant. Using conventional microbiology, high levels of Staphylococcus aureus carriage were observed, particularly in the 18–65 age group (n = 17/36; 47.2% 95% CI: 30.9–63.5). The highest carriage of pneumococci was in the <5 and 5 to 17 year olds, with 57.1% (4/7) and 49.2% (30/61), respectively. Sixteen pneumococcal serotypes were identified, the most common being the nonvaccine-type 23A (14.6%) and the vaccine-type 6B (9.8%). The prevalence of pneumococcal serotypes covered by pneumococcal conjugate vaccines support introduction into a Malaysian national immunisation schedule. In addition, the dominance of Corynebacterium in the airway microbiomes is intriguing given their role as a potentially protective commensal with respect to acute infection and respiratory health.https://doi.org/10.1038/s41522-020-00173-5 |
| spellingShingle | David W. Cleary Denise E. Morris Rebecca A. Anderson Jessica Jones Ahmed Ghazi Alattraqchi Nor Iza A. Rahman Salwani Ismail Mohd Sayuti Razali Rahmah Mohd Amin Aniza Abd Aziz Nor Kamaruzaman Esa Salman Amiruddin Ching Hoong Chew Hafis Simin Ramle Abdullah Chew Chieng Yeo Stuart C. Clarke The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia npj Biofilms and Microbiomes |
| title | The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia |
| title_full | The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia |
| title_fullStr | The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia |
| title_full_unstemmed | The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia |
| title_short | The upper respiratory tract microbiome of indigenous Orang Asli in north-eastern Peninsular Malaysia |
| title_sort | upper respiratory tract microbiome of indigenous orang asli in north eastern peninsular malaysia |
| url | https://doi.org/10.1038/s41522-020-00173-5 |
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