Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized
This study involves the isolation and characterization of a bacterial strain capable of bioleaching molybdenum ore. Bacterial growth was observed when rock sample was incubated in 9K at 70 ºC. The isolates were identified as extremely acidophilic, thermophilic and chemolithotroph archaebacteria. Fol...
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2017-08-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_28723_4d29461bee18ce5cbe5d6eb082448d44.pdf |
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| author | Mahshid Roshani Seyed Abbas Shojaosadati Seyed Jaber Safdari Ebrahim Vasheghani-Farahani Kazem Mirjalili Zahra Manafi |
| author_facet | Mahshid Roshani Seyed Abbas Shojaosadati Seyed Jaber Safdari Ebrahim Vasheghani-Farahani Kazem Mirjalili Zahra Manafi |
| author_sort | Mahshid Roshani |
| collection | DOAJ |
| description | This study involves the isolation and characterization of a bacterial strain capable of bioleaching molybdenum ore. Bacterial growth was observed when rock sample was incubated in 9K at 70 ºC. The isolates were identified as extremely acidophilic, thermophilic and chemolithotroph archaebacteria. Following PCR amplification of the 16S rDNA of the isolated strain, the sequencing of this region and comparison with the Gen-Bank database identified the strains as Acidianus ambivalens and Sulfolobus solfataricus. An experimental design was carried out to optimize bioleaching of molybdenum by these bacteria. Factors of pulp density, initial pH, the concentration of Fe3+ and the ratio of two bacteria are the variables and molybdenum and uranium recoveries were selected as responses. Bioleaching was carried out using molybdenum ore and pulp density of 4%, initial pH of 1.5, Fe3+ concentration of 11.5 g/L and Sulfolobus solfataricus to Acidianus ambivalens ratio of 2.0 were selected as optimum conditions. Molybdenum and uranium recoveries were 43.2% and 79.1% respectively. |
| first_indexed | 2024-12-11T17:32:45Z |
| format | Article |
| id | doaj.art-e207efd42c534ce6a088b4a8b27c9a37 |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-12-11T17:32:45Z |
| publishDate | 2017-08-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-e207efd42c534ce6a088b4a8b27c9a372022-12-22T00:56:46ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862017-08-0136418319428723Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and CharacterizedMahshid Roshani0Seyed Abbas Shojaosadati1Seyed Jaber Safdari2Ebrahim Vasheghani-Farahani3Kazem Mirjalili4Zahra Manafi5Biotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. IRANBiotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, IranNuclear Fuel Cycle Research School, Nuclear Science and Technology Research Institute, AEOI, Tehran, I.R. IRANBiotechnology Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, I.R. IRANNuclear Fuel Cycle Research School, Nuclear Science and Technology Research Institute, AEOI, Tehran, I.R. IRANDepartment of Microbiology, Islamic Azad University, Jahrom Branch, Jahrom, I.R. IRANThis study involves the isolation and characterization of a bacterial strain capable of bioleaching molybdenum ore. Bacterial growth was observed when rock sample was incubated in 9K at 70 ºC. The isolates were identified as extremely acidophilic, thermophilic and chemolithotroph archaebacteria. Following PCR amplification of the 16S rDNA of the isolated strain, the sequencing of this region and comparison with the Gen-Bank database identified the strains as Acidianus ambivalens and Sulfolobus solfataricus. An experimental design was carried out to optimize bioleaching of molybdenum by these bacteria. Factors of pulp density, initial pH, the concentration of Fe3+ and the ratio of two bacteria are the variables and molybdenum and uranium recoveries were selected as responses. Bioleaching was carried out using molybdenum ore and pulp density of 4%, initial pH of 1.5, Fe3+ concentration of 11.5 g/L and Sulfolobus solfataricus to Acidianus ambivalens ratio of 2.0 were selected as optimum conditions. Molybdenum and uranium recoveries were 43.2% and 79.1% respectively.http://www.ijcce.ac.ir/article_28723_4d29461bee18ce5cbe5d6eb082448d44.pdfbioleachingthermophilic bacteriamolybdenum extractionsulfolobus solfataricusacidianus ambivalens |
| spellingShingle | Mahshid Roshani Seyed Abbas Shojaosadati Seyed Jaber Safdari Ebrahim Vasheghani-Farahani Kazem Mirjalili Zahra Manafi Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized Iranian Journal of Chemistry & Chemical Engineering bioleaching thermophilic bacteria molybdenum extraction sulfolobus solfataricus acidianus ambivalens |
| title | Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized |
| title_full | Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized |
| title_fullStr | Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized |
| title_full_unstemmed | Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized |
| title_short | Bioleaching of Molybdenum by Two New Thermophilic Strains Isolated and Characterized |
| title_sort | bioleaching of molybdenum by two new thermophilic strains isolated and characterized |
| topic | bioleaching thermophilic bacteria molybdenum extraction sulfolobus solfataricus acidianus ambivalens |
| url | http://www.ijcce.ac.ir/article_28723_4d29461bee18ce5cbe5d6eb082448d44.pdf |
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