Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation
Abstract Background Microalgae CO2 fixation results in the production of biomass rich in high-valuable products, such as fatty acids and carotenoids. Enhanced productivity of valuable compounds can be achieved through the microalgae’s ability to capture CO2 efficiently from sources of high CO2 conte...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04042-z |
| _version_ | 1797952656361127936 |
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| author | Anastasiia Kryvenda Rudolf Tischner Bastian Steudel Carola Griehl Robert Armon Thomas Friedl |
| author_facet | Anastasiia Kryvenda Rudolf Tischner Bastian Steudel Carola Griehl Robert Armon Thomas Friedl |
| author_sort | Anastasiia Kryvenda |
| collection | DOAJ |
| description | Abstract Background Microalgae CO2 fixation results in the production of biomass rich in high-valuable products, such as fatty acids and carotenoids. Enhanced productivity of valuable compounds can be achieved through the microalgae’s ability to capture CO2 efficiently from sources of high CO2 contents, but it depends on the species. Culture collections of microalgae offer a wide variety of defined strains. However, an inadequate understanding of which groups of microalgae and from which habitats they originate offer high productivity under increased CO2 concentrations hampers exploiting microalgae as a sustainable source in the bioeconomy. Results A large variety of 81 defined algal strains, including new green algal isolates from various terrestrial environments, were studied for their growth under atmospheres with CO2 levels of 5–25% in air. They were from a pool of 200 strains that had been pre-selected for phylogenetic diversity and high productivity under ambient CO2. Green algae from terrestrial environments exhibited enhanced growth up to 25% CO2. In contrast, in unicellular red algae and stramenopile algae, which originated through the endosymbiotic uptake of a red algal cell, growth at CO2 concentrations above 5% was suppressed. While terrestrial stramenopile algae generally tolerated such CO2 concentrations, their counterparts from marine phytoplankton did not. The tests of four new strains in liquid culture revealed enhanced biomass and chlorophyll production under elevated CO2 levels. The 15% CO2 aeration increased their total carotenoid and fatty acid contents, which were further stimulated when combined with the starvation of macronutrients, i.e., less with phosphate and more with nitrogen-depleted culture media. Conclusion Green algae originating from terrestrial environments, Chlorophyceae and Trebouxiophyceae, exhibit enhanced productivity of carotenoids and fatty acids under elevated CO2 concentrations. This ability supports the economic and sustainable production of valuable compounds from these microalgae using inexpensive sources of high CO2 concentrations, such as industrial exhaust fumes. |
| first_indexed | 2024-04-10T22:49:52Z |
| format | Article |
| id | doaj.art-e20dbfac0d3d430d9603665ff1c30cd5 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-10T22:49:52Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-e20dbfac0d3d430d9603665ff1c30cd52023-01-15T12:08:11ZengBMCBMC Plant Biology1471-22292023-01-0123111710.1186/s12870-023-04042-zTesting for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitationAnastasiia Kryvenda0Rudolf Tischner1Bastian Steudel2Carola Griehl3Robert Armon4Thomas Friedl5Albrecht-von-Haller-Institute for Plant Sciences, Department of Experimental Phycology and Culture Collection of Algae (SAG), University of GöttingenAlbrecht-von-Haller-Institute for Plant Sciences, Department of Experimental Phycology and Culture Collection of Algae (SAG), University of GöttingenAlbrecht-von-Haller-Institute for Plant Sciences, Department of Experimental Phycology and Culture Collection of Algae (SAG), University of GöttingenDepartment of Applied Biosciences and Process Technology, Competence Center Algal Biotechnology, Anhalt University of Applied SciencesTechnion-Israel Institute of Technology, Faculty of Civil and Environmental EngineeringAlbrecht-von-Haller-Institute for Plant Sciences, Department of Experimental Phycology and Culture Collection of Algae (SAG), University of GöttingenAbstract Background Microalgae CO2 fixation results in the production of biomass rich in high-valuable products, such as fatty acids and carotenoids. Enhanced productivity of valuable compounds can be achieved through the microalgae’s ability to capture CO2 efficiently from sources of high CO2 contents, but it depends on the species. Culture collections of microalgae offer a wide variety of defined strains. However, an inadequate understanding of which groups of microalgae and from which habitats they originate offer high productivity under increased CO2 concentrations hampers exploiting microalgae as a sustainable source in the bioeconomy. Results A large variety of 81 defined algal strains, including new green algal isolates from various terrestrial environments, were studied for their growth under atmospheres with CO2 levels of 5–25% in air. They were from a pool of 200 strains that had been pre-selected for phylogenetic diversity and high productivity under ambient CO2. Green algae from terrestrial environments exhibited enhanced growth up to 25% CO2. In contrast, in unicellular red algae and stramenopile algae, which originated through the endosymbiotic uptake of a red algal cell, growth at CO2 concentrations above 5% was suppressed. While terrestrial stramenopile algae generally tolerated such CO2 concentrations, their counterparts from marine phytoplankton did not. The tests of four new strains in liquid culture revealed enhanced biomass and chlorophyll production under elevated CO2 levels. The 15% CO2 aeration increased their total carotenoid and fatty acid contents, which were further stimulated when combined with the starvation of macronutrients, i.e., less with phosphate and more with nitrogen-depleted culture media. Conclusion Green algae originating from terrestrial environments, Chlorophyceae and Trebouxiophyceae, exhibit enhanced productivity of carotenoids and fatty acids under elevated CO2 concentrations. This ability supports the economic and sustainable production of valuable compounds from these microalgae using inexpensive sources of high CO2 concentrations, such as industrial exhaust fumes.https://doi.org/10.1186/s12870-023-04042-zAlgaeChlorophyceaeTrebouxiophyceaeCarbon dioxideGrowthBiomass |
| spellingShingle | Anastasiia Kryvenda Rudolf Tischner Bastian Steudel Carola Griehl Robert Armon Thomas Friedl Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation BMC Plant Biology Algae Chlorophyceae Trebouxiophyceae Carbon dioxide Growth Biomass |
| title | Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation |
| title_full | Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation |
| title_fullStr | Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation |
| title_full_unstemmed | Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation |
| title_short | Testing for terrestrial and freshwater microalgae productivity under elevated CO2 conditions and nutrient limitation |
| title_sort | testing for terrestrial and freshwater microalgae productivity under elevated co2 conditions and nutrient limitation |
| topic | Algae Chlorophyceae Trebouxiophyceae Carbon dioxide Growth Biomass |
| url | https://doi.org/10.1186/s12870-023-04042-z |
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