Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals
Mammalian testes are very heterogeneous organs, with a high number of different cell types. Testicular heterogeneity, together with the lack of reliable in vitro culture systems of spermatogenic cells, have been an obstacle for the characterization of the molecular bases of the unique events that ta...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1151 |
| _version_ | 1797407866185842688 |
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| author | Rosana Rodríguez-Casuriaga Adriana Geisinger |
| author_facet | Rosana Rodríguez-Casuriaga Adriana Geisinger |
| author_sort | Rosana Rodríguez-Casuriaga |
| collection | DOAJ |
| description | Mammalian testes are very heterogeneous organs, with a high number of different cell types. Testicular heterogeneity, together with the lack of reliable in vitro culture systems of spermatogenic cells, have been an obstacle for the characterization of the molecular bases of the unique events that take place along the different spermatogenic stages. In this context, flow cytometry has become an invaluable tool for the analysis of testicular heterogeneity, and for the purification of stage-specific spermatogenic cell populations, both for basic research and for clinical applications. In this review, we highlight the importance of flow cytometry for the advances on the knowledge of the molecular groundwork of spermatogenesis in mammals. Moreover, we provide examples of different approaches to the study of spermatogenesis that have benefited from flow cytometry, including the characterization of mutant phenotypes, transcriptomics, epigenetic and genome-wide chromatin studies, and the attempts to establish cell culture systems for research and/or clinical aims such as infertility treatment. |
| first_indexed | 2024-03-09T03:48:55Z |
| format | Article |
| id | doaj.art-e20ec23a8f0a42bc99077c4325ff6aa4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:48:55Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e20ec23a8f0a42bc99077c4325ff6aa42023-12-03T14:31:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223115110.3390/ijms22031151Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in MammalsRosana Rodríguez-Casuriaga0Adriana Geisinger1Department of Molecular Biology, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 11600 Montevideo, UruguayDepartment of Molecular Biology, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), 11600 Montevideo, UruguayMammalian testes are very heterogeneous organs, with a high number of different cell types. Testicular heterogeneity, together with the lack of reliable in vitro culture systems of spermatogenic cells, have been an obstacle for the characterization of the molecular bases of the unique events that take place along the different spermatogenic stages. In this context, flow cytometry has become an invaluable tool for the analysis of testicular heterogeneity, and for the purification of stage-specific spermatogenic cell populations, both for basic research and for clinical applications. In this review, we highlight the importance of flow cytometry for the advances on the knowledge of the molecular groundwork of spermatogenesis in mammals. Moreover, we provide examples of different approaches to the study of spermatogenesis that have benefited from flow cytometry, including the characterization of mutant phenotypes, transcriptomics, epigenetic and genome-wide chromatin studies, and the attempts to establish cell culture systems for research and/or clinical aims such as infertility treatment.https://www.mdpi.com/1422-0067/22/3/1151spermatogenesisflow cytometryFACSmale infertility |
| spellingShingle | Rosana Rodríguez-Casuriaga Adriana Geisinger Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals International Journal of Molecular Sciences spermatogenesis flow cytometry FACS male infertility |
| title | Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals |
| title_full | Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals |
| title_fullStr | Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals |
| title_full_unstemmed | Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals |
| title_short | Contributions of Flow Cytometry to the Molecular Study of Spermatogenesis in Mammals |
| title_sort | contributions of flow cytometry to the molecular study of spermatogenesis in mammals |
| topic | spermatogenesis flow cytometry FACS male infertility |
| url | https://www.mdpi.com/1422-0067/22/3/1151 |
| work_keys_str_mv | AT rosanarodriguezcasuriaga contributionsofflowcytometrytothemolecularstudyofspermatogenesisinmammals AT adrianageisinger contributionsofflowcytometrytothemolecularstudyofspermatogenesisinmammals |