Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8
A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium <i>Thermus thermophilus</i> HB8 as its host. Phage φOH3, which was classified into the <i>Inoviridae</i> family, consists of a flexible f...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00050/full |
| _version_ | 1811315010819850240 |
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| author | Yuko eNagayoshi Kenta eKumagae Kazuki eMori Kosuke eTashiro Ayano eNakamura Yasuhiro eFujino Yasuaki eHiromasa Takeo eIwamoto Satoru eKuhara Toshihisa eOhshima Katsumi eDoi |
| author_facet | Yuko eNagayoshi Kenta eKumagae Kazuki eMori Kosuke eTashiro Ayano eNakamura Yasuhiro eFujino Yasuaki eHiromasa Takeo eIwamoto Satoru eKuhara Toshihisa eOhshima Katsumi eDoi |
| author_sort | Yuko eNagayoshi |
| collection | DOAJ |
| description | A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium <i>Thermus thermophilus</i> HB8 as its host. Phage φOH3, which was classified into the <i>Inoviridae</i> family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90 °C and at pHs ranging from 6 to 9. A one-step growth curve of the phage showed a 60-min latent period beginning immediately postinfection, followed by intracellular virus particle production during the subsequent 40 min. The released virion number of φOH3 was 109. During the latent period, both single stranded DNA (ssDNA) and the replicative form (RF) of phage DNA were multiplied from min 40 onward. During the release period, the copy numbers of both ssDNA and RF DNA increased sharply. The size of the φOH3 genome is 5,688 bp, and 8 putative open reading frames (ORFs) were annotated. These ORFs were encoded on the plus strand of RF DNA and showed no significant homology with any known phage genes, except ORF 5, which showed 60% identity with the gene VIII product of the <i>Thermus</i> filamentous phage PH75. All the ORFs were similar to predicted genes annotated in the <i>Thermus aquaticus</i> Y51MC23 and <i>Meiothermus timidus</i> DSM 17022 genomes at the amino acid sequence level. This is the first report of the whole genome structure and DNA multiplication of a filamentous <i>T. thermophilus</i> phage within its host cell. |
| first_indexed | 2024-04-13T11:22:39Z |
| format | Article |
| id | doaj.art-e2139052e0664e2399bd4669066b6492 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T11:22:39Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-e2139052e0664e2399bd4669066b64922022-12-22T02:48:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-02-01710.3389/fmicb.2016.00050169192Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8Yuko eNagayoshi0Kenta eKumagae1Kazuki eMori2Kosuke eTashiro3Ayano eNakamura4Yasuhiro eFujino5Yasuaki eHiromasa6Takeo eIwamoto7Satoru eKuhara8Toshihisa eOhshima9Katsumi eDoi10Kyushu UniversityKyushu UniversityKyushu UniversityKyushu UniversityKyushu UniversityKyushu UniversityKyushu UniversityJikei University School of MedicineKyushu UniversityOsaka Institute of TechnologyKyushu UniversityA filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium <i>Thermus thermophilus</i> HB8 as its host. Phage φOH3, which was classified into the <i>Inoviridae</i> family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90 °C and at pHs ranging from 6 to 9. A one-step growth curve of the phage showed a 60-min latent period beginning immediately postinfection, followed by intracellular virus particle production during the subsequent 40 min. The released virion number of φOH3 was 109. During the latent period, both single stranded DNA (ssDNA) and the replicative form (RF) of phage DNA were multiplied from min 40 onward. During the release period, the copy numbers of both ssDNA and RF DNA increased sharply. The size of the φOH3 genome is 5,688 bp, and 8 putative open reading frames (ORFs) were annotated. These ORFs were encoded on the plus strand of RF DNA and showed no significant homology with any known phage genes, except ORF 5, which showed 60% identity with the gene VIII product of the <i>Thermus</i> filamentous phage PH75. All the ORFs were similar to predicted genes annotated in the <i>Thermus aquaticus</i> Y51MC23 and <i>Meiothermus timidus</i> DSM 17022 genomes at the amino acid sequence level. This is the first report of the whole genome structure and DNA multiplication of a filamentous <i>T. thermophilus</i> phage within its host cell.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00050/fullInoviridaeThermus thermophilusfilamentous phageReplicative formHyperthermophilic phage |
| spellingShingle | Yuko eNagayoshi Kenta eKumagae Kazuki eMori Kosuke eTashiro Ayano eNakamura Yasuhiro eFujino Yasuaki eHiromasa Takeo eIwamoto Satoru eKuhara Toshihisa eOhshima Katsumi eDoi Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 Frontiers in Microbiology Inoviridae Thermus thermophilus filamentous phage Replicative form Hyperthermophilic phage |
| title | Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 |
| title_full | Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 |
| title_fullStr | Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 |
| title_full_unstemmed | Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 |
| title_short | Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects <i>Thermus thermophilu</i>s HB8 |
| title_sort | physiological properties and genome structure of the hyperthermophilic filamentous phage φoh3 which infects lt i gt thermus thermophilu lt i gt s hb8 |
| topic | Inoviridae Thermus thermophilus filamentous phage Replicative form Hyperthermophilic phage |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00050/full |
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