Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia
Abstract Background Stenotrophomonas maltophilia is able to cause infections in immunocompromised patients, and the treatment of this opportunistic pathogen is complicated due to its virulence factors, antibiotic resistance, and the ability of the bacteria to produce biofilm. The main goals of this...
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| Format: | Article |
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BMC
2023-09-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-03008-3 |
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| author | Mohadeseh Ostovari Deilamani Farhad Nikkhahi Mehdi Bakht Safar Ali Alizadeh Fatemeh Fardsanei Amir Javadi Seyed Mahmoud Amin Marashi Masoumeh Aslanimehr Amir Peymani |
| author_facet | Mohadeseh Ostovari Deilamani Farhad Nikkhahi Mehdi Bakht Safar Ali Alizadeh Fatemeh Fardsanei Amir Javadi Seyed Mahmoud Amin Marashi Masoumeh Aslanimehr Amir Peymani |
| author_sort | Mohadeseh Ostovari Deilamani |
| collection | DOAJ |
| description | Abstract Background Stenotrophomonas maltophilia is able to cause infections in immunocompromised patients, and the treatment of this opportunistic pathogen is complicated due to its virulence factors, antibiotic resistance, and the ability of the bacteria to produce biofilm. The main goals of this study were to assess the susceptibility of extensively drug-resistant (XDR) isolates to ethanol and EDTA, and evaluating the synergistic effect of these disinfectants, and also survey the effect of exposure to sub-inhibitory concentrations of ethanol and EDTA on the expression of biofilm-producing smf-1, rpfF genes. Results The results showed that EDTA significantly increased the effectiveness of the ethanol and have a synergistic effect. All of the 10 XDR isolates included in the current study harbored smf-1 and rpfF genes and produced biofilm. After exposure to MIC, sub-MIC, synergism, and sub-synergism of ethanol and EDTA, the expression of smf-1 and rpfF genes was repressed significantly. Conclusion In the current study, it was indicated that the expression of biofilm-producing genes was repressed when bacteria are exposed to different concentrations of ethanol and EDTA. Future studies should include more complex microbial communities residing in the hospitals, and more disinfectants use in hospitals. Expression of other virulence genes in different conditions is suggested. |
| first_indexed | 2024-03-09T15:26:46Z |
| format | Article |
| id | doaj.art-e2178957aa9e479c8c667cdf612fc16e |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T15:26:46Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-e2178957aa9e479c8c667cdf612fc16e2023-11-26T12:30:17ZengBMCBMC Microbiology1471-21802023-09-0123111210.1186/s12866-023-03008-3Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophiliaMohadeseh Ostovari Deilamani0Farhad Nikkhahi1Mehdi Bakht2Safar Ali Alizadeh3Fatemeh Fardsanei4Amir Javadi5Seyed Mahmoud Amin Marashi6Masoumeh Aslanimehr7Amir Peymani8Medical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesMedical Microbiology Research Center, Qazvin University of Medical SciencesAbstract Background Stenotrophomonas maltophilia is able to cause infections in immunocompromised patients, and the treatment of this opportunistic pathogen is complicated due to its virulence factors, antibiotic resistance, and the ability of the bacteria to produce biofilm. The main goals of this study were to assess the susceptibility of extensively drug-resistant (XDR) isolates to ethanol and EDTA, and evaluating the synergistic effect of these disinfectants, and also survey the effect of exposure to sub-inhibitory concentrations of ethanol and EDTA on the expression of biofilm-producing smf-1, rpfF genes. Results The results showed that EDTA significantly increased the effectiveness of the ethanol and have a synergistic effect. All of the 10 XDR isolates included in the current study harbored smf-1 and rpfF genes and produced biofilm. After exposure to MIC, sub-MIC, synergism, and sub-synergism of ethanol and EDTA, the expression of smf-1 and rpfF genes was repressed significantly. Conclusion In the current study, it was indicated that the expression of biofilm-producing genes was repressed when bacteria are exposed to different concentrations of ethanol and EDTA. Future studies should include more complex microbial communities residing in the hospitals, and more disinfectants use in hospitals. Expression of other virulence genes in different conditions is suggested.https://doi.org/10.1186/s12866-023-03008-3Nosocomial infectionBiofilmStenotrophomonas maltophiliaClinical isolatesEDTAEthanol |
| spellingShingle | Mohadeseh Ostovari Deilamani Farhad Nikkhahi Mehdi Bakht Safar Ali Alizadeh Fatemeh Fardsanei Amir Javadi Seyed Mahmoud Amin Marashi Masoumeh Aslanimehr Amir Peymani Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia BMC Microbiology Nosocomial infection Biofilm Stenotrophomonas maltophilia Clinical isolates EDTA Ethanol |
| title | Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia |
| title_full | Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia |
| title_fullStr | Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia |
| title_full_unstemmed | Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia |
| title_short | Evaluation of ethanol and EDTA concentrations in the expression of biofilm-producing smf-1, rpfF genes in XDR clinical isolates of Stenotrophomonas maltophilia |
| title_sort | evaluation of ethanol and edta concentrations in the expression of biofilm producing smf 1 rpff genes in xdr clinical isolates of stenotrophomonas maltophilia |
| topic | Nosocomial infection Biofilm Stenotrophomonas maltophilia Clinical isolates EDTA Ethanol |
| url | https://doi.org/10.1186/s12866-023-03008-3 |
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