In vitro blastocyst development of post-thaw vitrified bovine oocytes
Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro.Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in me...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2013-08-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.6/Oct-2013/7.pdf |
| Summary: | Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro.Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in medium TCM-199 with 10% FBS. Immediately, within a minute they are plunged into liquid nitrogen using 0.25 ml straws. Thawing was made with a step wise dilution method. Post-thaw normal vitrified and non-vitrified oocytes were subjected to in vitro maturation and in vitro fertilization.Results: Post-thaw survival percentage of vitrified oocytes was 88.37% and maturation performance of vitrified oocytes on the basis of cumulus expansion was 81.58% as compared to non-vitrified control 93.85%. The in vitro fertilization performance of vitrified oocytes was 49.46% as compared to the non-vitrified ones (63.11%). Similarly, blastocyst formation of vitrified oocytes was 21.74% as compared to 32.47% in non-vitrified oocytes.Conclusion: Vitrification of immature bovine oocytes using 7.5% EG + 7.5% DMSO for equilibration and 15% EG +15% DMSO + 0.6 M sucrose as vitrification solution yielded better in vitro fertilization and blastocyst formation rate. |
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| ISSN: | 0972-8988 2231-0916 |