Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae
Gang Wang,* Guobin Song,* Yuanhong Xu Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuanhong Xu Email xyhong1964@163.comBackground...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-06-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/association-of-crisprcas-system-with-the-drug-resistance-in-klebsiella-peer-reviewed-article-IDR |
| _version_ | 1818969838996946944 |
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| author | Wang G Song G Xu Y |
| author_facet | Wang G Song G Xu Y |
| author_sort | Wang G |
| collection | DOAJ |
| description | Gang Wang,* Guobin Song,* Yuanhong Xu Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuanhong Xu Email xyhong1964@163.comBackground: Klebsiella pneumoniae is a common opportunistic pathogen and its production of extended-spectrum β-lactamases (ESBL) and carbapenemases leads to drug resistance. Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated genes (Cas) are widespread in the genome of many bacteria and are a defense mechanism against foreign invaders such as plasmids and viruses.Purpose: To investigate the prevalence of the CRISPR/Cas system in wild type strains of K. pneumoniae in the hospital and its association with drug resistance.Materials and Methods: A total of 136 strains were collected and characterized their susceptibility to antimicrobial agents. The prevalence of CRISPR/Cas system was detected by PCR and DNA sequencing was analyzed by CRISPRFinder. The statistical analysis of the results was performed by SPSS.Results: We found that 50/136 (37%) isolates produced ESBL and 30/136 (22%) isolates were resistant to carbapenems. These isolates were liable to be multidrug resistant against β-lactams, quinolones, and aminoglycosides. Among the carbapenem-resistant isolates, blaKPC was the main drug resistance-associated gene and different types of ESBL and AmpC genes were present. Resistance to β-lactams, quinolones, aminoglycosides, tetracyclines, and β-lactams/enzyme inhibitor were higher in absence of the CRISPR/Cas system. Eighteen spacers within the CRISPR arrays matched with the genomes of plasmids or phages, some of which carried drug resistance genes.Conclusion: ESBL-producing and carbapenem-resistant K. pneumoniae are more likely to develop multidrug resistance and show an inverse correlation between drug resistance and CRISPR/Cas system. Absence of CRISPR/Cas modules allow for the acquisition of external drug resistance genes.Keywords: CRISPR/Cas, Klebsiella pneumoniae, extended-spectrum β-lactamases, carbapenemases, drug resistance |
| first_indexed | 2024-12-20T14:26:57Z |
| format | Article |
| id | doaj.art-e219d6216d27419a94fa2d47f88131f4 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-20T14:26:57Z |
| publishDate | 2020-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-e219d6216d27419a94fa2d47f88131f42022-12-21T19:37:46ZengDove Medical PressInfection and Drug Resistance1178-69732020-06-01Volume 131929193554737Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniaeWang GSong GXu YGang Wang,* Guobin Song,* Yuanhong Xu Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yuanhong Xu Email xyhong1964@163.comBackground: Klebsiella pneumoniae is a common opportunistic pathogen and its production of extended-spectrum β-lactamases (ESBL) and carbapenemases leads to drug resistance. Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated genes (Cas) are widespread in the genome of many bacteria and are a defense mechanism against foreign invaders such as plasmids and viruses.Purpose: To investigate the prevalence of the CRISPR/Cas system in wild type strains of K. pneumoniae in the hospital and its association with drug resistance.Materials and Methods: A total of 136 strains were collected and characterized their susceptibility to antimicrobial agents. The prevalence of CRISPR/Cas system was detected by PCR and DNA sequencing was analyzed by CRISPRFinder. The statistical analysis of the results was performed by SPSS.Results: We found that 50/136 (37%) isolates produced ESBL and 30/136 (22%) isolates were resistant to carbapenems. These isolates were liable to be multidrug resistant against β-lactams, quinolones, and aminoglycosides. Among the carbapenem-resistant isolates, blaKPC was the main drug resistance-associated gene and different types of ESBL and AmpC genes were present. Resistance to β-lactams, quinolones, aminoglycosides, tetracyclines, and β-lactams/enzyme inhibitor were higher in absence of the CRISPR/Cas system. Eighteen spacers within the CRISPR arrays matched with the genomes of plasmids or phages, some of which carried drug resistance genes.Conclusion: ESBL-producing and carbapenem-resistant K. pneumoniae are more likely to develop multidrug resistance and show an inverse correlation between drug resistance and CRISPR/Cas system. Absence of CRISPR/Cas modules allow for the acquisition of external drug resistance genes.Keywords: CRISPR/Cas, Klebsiella pneumoniae, extended-spectrum β-lactamases, carbapenemases, drug resistancehttps://www.dovepress.com/association-of-crisprcas-system-with-the-drug-resistance-in-klebsiella-peer-reviewed-article-IDRcrispr/casklebsiella pneumoniaeextended-spectrum β-lactamasescarbapenemasesdrug resistance. |
| spellingShingle | Wang G Song G Xu Y Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae Infection and Drug Resistance crispr/cas klebsiella pneumoniae extended-spectrum β-lactamases carbapenemases drug resistance. |
| title | Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae |
| title_full | Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae |
| title_fullStr | Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae |
| title_full_unstemmed | Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae |
| title_short | Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae |
| title_sort | association of crispr cas system with the drug resistance in klebsiella pneumoniae |
| topic | crispr/cas klebsiella pneumoniae extended-spectrum β-lactamases carbapenemases drug resistance. |
| url | https://www.dovepress.com/association-of-crisprcas-system-with-the-drug-resistance-in-klebsiella-peer-reviewed-article-IDR |
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