Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor
Enzyme modified electrodes were fabricated with poly(aniline-co-m-aminophenol). Electrochemical polymerization of aniline and m-aminophenol wasperformed to get the film of copolymer on the surface of gold electrode. Modifiedelectrodes were fabricated by two methods, physical entrapment and covalent...
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| Format: | Article |
| Language: | English |
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MDPI AG
2007-05-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/7/5/719/ |
| _version_ | 1811279193299746816 |
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| author | Seong-Ho Choi Yong Taik Lim Sang J. Chung Anantha Iyengar Gopalan Kwang-Pill Lee Kang-Deuk Seo |
| author_facet | Seong-Ho Choi Yong Taik Lim Sang J. Chung Anantha Iyengar Gopalan Kwang-Pill Lee Kang-Deuk Seo |
| author_sort | Seong-Ho Choi |
| collection | DOAJ |
| description | Enzyme modified electrodes were fabricated with poly(aniline-co-m-aminophenol). Electrochemical polymerization of aniline and m-aminophenol wasperformed to get the film of copolymer on the surface of gold electrode. Modifiedelectrodes were fabricated by two methods, physical entrapment and covalent cross-linking.In one of the method, gold nanoparticles were loaded into the copolymer film andhorseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymerfilm through physical entrapment. In the other method, the amino and -OH groups in thecopolymer are utilized to form covalent functionalization with HRP via glutaric dialdehydeas cross-linker/mediator. The conducting copolymer/enzyme modified electrodes preparedby physical entrapment/covalent functionalization of enzyme were tested forelectrocatalytic activities towards sensing of H2O2. Amperometric results indicate thatenzyme modified electrode via physical entrapment possesses better electrocatalyticperformance over covalent functionalized enzyme electrode. |
| first_indexed | 2024-04-13T00:50:18Z |
| format | Article |
| id | doaj.art-e21a212857094b3e8ff49c17fe9ef640 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T00:50:18Z |
| publishDate | 2007-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-e21a212857094b3e8ff49c17fe9ef6402022-12-22T03:09:53ZengMDPI AGSensors1424-82202007-05-017571972910.3390/s7050719Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide SensorSeong-Ho ChoiYong Taik LimSang J. ChungAnantha Iyengar GopalanKwang-Pill LeeKang-Deuk SeoEnzyme modified electrodes were fabricated with poly(aniline-co-m-aminophenol). Electrochemical polymerization of aniline and m-aminophenol wasperformed to get the film of copolymer on the surface of gold electrode. Modifiedelectrodes were fabricated by two methods, physical entrapment and covalent cross-linking.In one of the method, gold nanoparticles were loaded into the copolymer film andhorseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymerfilm through physical entrapment. In the other method, the amino and -OH groups in thecopolymer are utilized to form covalent functionalization with HRP via glutaric dialdehydeas cross-linker/mediator. The conducting copolymer/enzyme modified electrodes preparedby physical entrapment/covalent functionalization of enzyme were tested forelectrocatalytic activities towards sensing of H2O2. Amperometric results indicate thatenzyme modified electrode via physical entrapment possesses better electrocatalyticperformance over covalent functionalized enzyme electrode.http://www.mdpi.com/1424-8220/7/5/719/horseradish peroxidasepoly(aniline-co-m-aminophenol)Au nanoparticlesphysical adsorptioncovalent bonding |
| spellingShingle | Seong-Ho Choi Yong Taik Lim Sang J. Chung Anantha Iyengar Gopalan Kwang-Pill Lee Kang-Deuk Seo Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor Sensors horseradish peroxidase poly(aniline-co-m-aminophenol) Au nanoparticles physical adsorption covalent bonding |
| title | Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor |
| title_full | Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor |
| title_fullStr | Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor |
| title_full_unstemmed | Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor |
| title_short | Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor |
| title_sort | horseradish peroxidase hrp immobilized poly aniline co m aminophenol film electrodesa¢a€a fabrication and evaluation as hydrogen peroxide sensor |
| topic | horseradish peroxidase poly(aniline-co-m-aminophenol) Au nanoparticles physical adsorption covalent bonding |
| url | http://www.mdpi.com/1424-8220/7/5/719/ |
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