New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering
Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with...
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MDPI AG
2020-07-01
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| Online Access: | https://www.mdpi.com/1996-1944/13/14/3149 |
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| author | Maria Râpă Carmen Gaidău Laura Mihaela Stefan Ecaterina Matei Mihaela Niculescu Mariana Daniela Berechet Maria Stanca Cristina Tablet Mădălina Tudorache Raluca Gavrilă Cristian Predescu Ruxandra Vidu |
| author_facet | Maria Râpă Carmen Gaidău Laura Mihaela Stefan Ecaterina Matei Mihaela Niculescu Mariana Daniela Berechet Maria Stanca Cristina Tablet Mădălina Tudorache Raluca Gavrilă Cristian Predescu Ruxandra Vidu |
| author_sort | Maria Râpă |
| collection | DOAJ |
| description | Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering. |
| first_indexed | 2024-03-10T18:28:06Z |
| format | Article |
| id | doaj.art-e21a943207104f3c9a2bcb0c8e5f8550 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T18:28:06Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-e21a943207104f3c9a2bcb0c8e5f85502023-11-20T06:51:23ZengMDPI AGMaterials1996-19442020-07-011314314910.3390/ma13143149New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue EngineeringMaria Râpă0Carmen Gaidău1Laura Mihaela Stefan2Ecaterina Matei3Mihaela Niculescu4Mariana Daniela Berechet5Maria Stanca6Cristina Tablet7Mădălina Tudorache8Raluca Gavrilă9Cristian Predescu10Ruxandra Vidu11Faculty of Material Sciences and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, RomaniaNational Research and Development Institute for Textiles and Leather- Division Leather and Footwear Research Institute, 031215 Bucharest, RomaniaNational Institute of Research and Development for Biological Sciences, 296 Splaiul Independenţei, 060031 Bucharest, RomaniaFaculty of Material Sciences and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, RomaniaNational Research and Development Institute for Textiles and Leather- Division Leather and Footwear Research Institute, 031215 Bucharest, RomaniaNational Research and Development Institute for Textiles and Leather- Division Leather and Footwear Research Institute, 031215 Bucharest, RomaniaNational Research and Development Institute for Textiles and Leather- Division Leather and Footwear Research Institute, 031215 Bucharest, RomaniaDepartment of Physical Chemistry, University of Bucharest, 4–12 Blvd. Regina Elisabeta, 030018 Bucharest, RomaniaDepartment of Physical Chemistry, University of Bucharest, 4–12 Blvd. Regina Elisabeta, 030018 Bucharest, RomaniaNano-scale Structuring and Characterization Laboratory, National Institute for R&D in Microtechnologies, 126A Erou Iancu Nicolae Street, R-077190 Voluntari, RomaniaFaculty of Material Sciences and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, RomaniaFaculty of Material Sciences and Engineering, Politehnica University of Bucharest, 313 Spl. Independentei, 060042 Bucharest, RomaniaConcentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.https://www.mdpi.com/1996-1944/13/14/3149collagen hydrolysaterabbit collagen gluekeratin hydrolysateelectrospinningtriple helixin vitro cytotoxicity |
| spellingShingle | Maria Râpă Carmen Gaidău Laura Mihaela Stefan Ecaterina Matei Mihaela Niculescu Mariana Daniela Berechet Maria Stanca Cristina Tablet Mădălina Tudorache Raluca Gavrilă Cristian Predescu Ruxandra Vidu New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering Materials collagen hydrolysate rabbit collagen glue keratin hydrolysate electrospinning triple helix in vitro cytotoxicity |
| title | New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering |
| title_full | New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering |
| title_fullStr | New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering |
| title_full_unstemmed | New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering |
| title_short | New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering |
| title_sort | new nanofibers based on protein by products with bioactive potential for tissue engineering |
| topic | collagen hydrolysate rabbit collagen glue keratin hydrolysate electrospinning triple helix in vitro cytotoxicity |
| url | https://www.mdpi.com/1996-1944/13/14/3149 |
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