Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice
Background: Zhumeria majdae showed antinociceptive and anti-inflammatory activity. The antinociceptive effect was inhibited by naloxone in hot-plate test. Objective: As Z. majdae antinociceptive activity was inhibited by an opioid antagonist, we decided to evaluate the effects of this plant on withd...
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| Format: | Article |
| Language: | English |
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Institue of Medicinal Plants, ACECR
2007-03-01
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| Series: | Journal of Medicinal Plants |
| Subjects: | |
| Online Access: | http://jmp.ir/article-1-641-en.html |
| _version_ | 1819101638288211968 |
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| author | H Hosseinzadeh M Ramezani M Ghorbani |
| author_facet | H Hosseinzadeh M Ramezani M Ghorbani |
| author_sort | H Hosseinzadeh |
| collection | DOAJ |
| description | Background: Zhumeria majdae showed antinociceptive and anti-inflammatory activity. The antinociceptive effect was inhibited by naloxone in hot-plate test. Objective: As Z. majdae antinociceptive activity was inhibited by an opioid antagonist, we decided to evaluate the effects of this plant on withdrawal syndrome. Methods: Dependence was induced using subcutaneous injections of morphine daily for three days (50, 50 and 75 mg/kg). On the fourth day, morphine was injected two hours prior to the intraperitoneal injection of naloxone. The number of jumps during the 30 minutes period after naloxone injection was considered as measure of withdrawal syndrome. The extracts and fractions were injected 30 min prior to morphine injection. The results indicated that methanolic extracts (0.28, 1.12 and 1.96 mg/kg) reduced the number of jumps (compared to control) up to %64, %85 and % 99.3, respectively.The methanolic-aqueous (3:2) extracted with chlorphorm also reduced withdrawal syndrome. Three MPLC fractions, 0.28 mg/kg, (A, B and C, similar Rf in each group) also reduced the number of jumping up to %97, %92 and %55, respectively in mice. In the open field test, on the contrary of MPLC fractions, the methanolic extract reduced locomotor activity. Conclusion: It is concluded that Z. majdae aerial parts methanolic extract and MPLC fractions reduced withdrawal syndrome via differents mechanism of actions. |
| first_indexed | 2024-12-22T01:21:51Z |
| format | Article |
| id | doaj.art-e21c36c1f8ae4324890a04308269562e |
| institution | Directory Open Access Journal |
| issn | 2717-204X 2717-2058 |
| language | English |
| last_indexed | 2024-12-22T01:21:51Z |
| publishDate | 2007-03-01 |
| publisher | Institue of Medicinal Plants, ACECR |
| record_format | Article |
| series | Journal of Medicinal Plants |
| spelling | doaj.art-e21c36c1f8ae4324890a04308269562e2022-12-21T18:43:42ZengInstitue of Medicinal Plants, ACECRJournal of Medicinal Plants2717-204X2717-20582007-03-016214860Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in MiceH Hosseinzadeh0M Ramezani1M Ghorbani2 1- Pharmacology and Toxicology Department, Pharmaceutical Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 2- Pharmacognosy and Biotechnology Department, Pharmaceutical Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 3- Pharmacist Background: Zhumeria majdae showed antinociceptive and anti-inflammatory activity. The antinociceptive effect was inhibited by naloxone in hot-plate test. Objective: As Z. majdae antinociceptive activity was inhibited by an opioid antagonist, we decided to evaluate the effects of this plant on withdrawal syndrome. Methods: Dependence was induced using subcutaneous injections of morphine daily for three days (50, 50 and 75 mg/kg). On the fourth day, morphine was injected two hours prior to the intraperitoneal injection of naloxone. The number of jumps during the 30 minutes period after naloxone injection was considered as measure of withdrawal syndrome. The extracts and fractions were injected 30 min prior to morphine injection. The results indicated that methanolic extracts (0.28, 1.12 and 1.96 mg/kg) reduced the number of jumps (compared to control) up to %64, %85 and % 99.3, respectively.The methanolic-aqueous (3:2) extracted with chlorphorm also reduced withdrawal syndrome. Three MPLC fractions, 0.28 mg/kg, (A, B and C, similar Rf in each group) also reduced the number of jumping up to %97, %92 and %55, respectively in mice. In the open field test, on the contrary of MPLC fractions, the methanolic extract reduced locomotor activity. Conclusion: It is concluded that Z. majdae aerial parts methanolic extract and MPLC fractions reduced withdrawal syndrome via differents mechanism of actions.http://jmp.ir/article-1-641-en.htmlzhumeria majdaewithdrawal syndromefractionmorphineopioid system |
| spellingShingle | H Hosseinzadeh M Ramezani M Ghorbani Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice Journal of Medicinal Plants zhumeria majdae withdrawal syndrome fraction morphine opioid system |
| title | Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice |
| title_full | Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice |
| title_fullStr | Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice |
| title_full_unstemmed | Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice |
| title_short | Effect of Zhumeria majdae Rech. f. & Wendelbo Aerial Parts Extracts and Fractions on Morphine Withdrawal Syndrome in Mice |
| title_sort | effect of zhumeria majdae rech f wendelbo aerial parts extracts and fractions on morphine withdrawal syndrome in mice |
| topic | zhumeria majdae withdrawal syndrome fraction morphine opioid system |
| url | http://jmp.ir/article-1-641-en.html |
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