INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION
ABSTRACT Background: The role of autonomic nervous system in the development and maintenance of portal hypertension is not fully elucidated. It is known that the gene expression of norepinephrine in the superior mesenteric artery varies with time, and it may contribute for splanchnic vasodilation a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Colégio Brasileiro de Cirurgia Digestiva
2018-08-01
|
| Series: | ABCD: Arquivos Brasileiros de Cirurgia Digestiva |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202018000300301&tlng=pt |
| _version_ | 1818732103376830464 |
|---|---|
| author | Antonella VINHOLI Marília Da Cruz FAGUNDES Danieli Cristina PIGOZZO Fernando Bermudez KUBRUSLY Luiz Fernando KUBRUSLY Camila Aparecida Moraes MARQUES |
| author_facet | Antonella VINHOLI Marília Da Cruz FAGUNDES Danieli Cristina PIGOZZO Fernando Bermudez KUBRUSLY Luiz Fernando KUBRUSLY Camila Aparecida Moraes MARQUES |
| author_sort | Antonella VINHOLI |
| collection | DOAJ |
| description | ABSTRACT Background: The role of autonomic nervous system in the development and maintenance of portal hypertension is not fully elucidated. It is known that the gene expression of norepinephrine in the superior mesenteric artery varies with time, and it may contribute for splanchnic vasodilation and its consequent hemodynamic repercussions. It is still not known exactly how the adrenergic expression behaves at the heart level in the initial stages of this process. Aim: To evaluate the immunohistochemical expression of the enzyme tyrosine hydroxylase (tyrosine 3-monooxygenase), involved in the synthesis of norepinephrine, in the myocardium of rats submitted to partial ligation of the portal vein. Methods: Twenty-four Wistar rats were divided into two groups: Sham Operated and Portal Hypertension. The partial ligation was performed in the Portal Hypertension group, and after 1/6/24 h and 3/5/14 days the animals were euthanized. Immunohistochemical analysis was performed to quantify the expression of the stained enzyme using the ImageJ program. Results: The Portal Hypertension group expressed percentages between 4.6-6% of the marked area, while the Sham Operated group varied between 4-5%. Although there was no statistical significance, the percentage stained in the Portal Hypertension group followed an increasing pattern in the first 6 h and a decreasing pattern after 24 h, which was not observed in the Sham Operated group. Conclusion: The expression of noradrenaline in rat myocardium during the first two weeks after partial ligation of the portal vein, with tyrosine hydroxylase as marker, did not show differences between groups over time. |
| first_indexed | 2024-12-17T23:28:15Z |
| format | Article |
| id | doaj.art-e21e41ee01e043dfb387af5c1bf9b584 |
| institution | Directory Open Access Journal |
| issn | 0102-6720 |
| language | English |
| last_indexed | 2024-12-17T23:28:15Z |
| publishDate | 2018-08-01 |
| publisher | Colégio Brasileiro de Cirurgia Digestiva |
| record_format | Article |
| series | ABCD: Arquivos Brasileiros de Cirurgia Digestiva |
| spelling | doaj.art-e21e41ee01e043dfb387af5c1bf9b5842022-12-21T21:28:43ZengColégio Brasileiro de Cirurgia DigestivaABCD: Arquivos Brasileiros de Cirurgia Digestiva0102-67202018-08-0131310.1590/0102-672020180001e1383INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSIONAntonella VINHOLIMarília Da Cruz FAGUNDESDanieli Cristina PIGOZZOFernando Bermudez KUBRUSLYLuiz Fernando KUBRUSLYCamila Aparecida Moraes MARQUESABSTRACT Background: The role of autonomic nervous system in the development and maintenance of portal hypertension is not fully elucidated. It is known that the gene expression of norepinephrine in the superior mesenteric artery varies with time, and it may contribute for splanchnic vasodilation and its consequent hemodynamic repercussions. It is still not known exactly how the adrenergic expression behaves at the heart level in the initial stages of this process. Aim: To evaluate the immunohistochemical expression of the enzyme tyrosine hydroxylase (tyrosine 3-monooxygenase), involved in the synthesis of norepinephrine, in the myocardium of rats submitted to partial ligation of the portal vein. Methods: Twenty-four Wistar rats were divided into two groups: Sham Operated and Portal Hypertension. The partial ligation was performed in the Portal Hypertension group, and after 1/6/24 h and 3/5/14 days the animals were euthanized. Immunohistochemical analysis was performed to quantify the expression of the stained enzyme using the ImageJ program. Results: The Portal Hypertension group expressed percentages between 4.6-6% of the marked area, while the Sham Operated group varied between 4-5%. Although there was no statistical significance, the percentage stained in the Portal Hypertension group followed an increasing pattern in the first 6 h and a decreasing pattern after 24 h, which was not observed in the Sham Operated group. Conclusion: The expression of noradrenaline in rat myocardium during the first two weeks after partial ligation of the portal vein, with tyrosine hydroxylase as marker, did not show differences between groups over time.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202018000300301&tlng=ptPortal HypertensionPortal veinLigationTyrosine 3-monooxygenaseSympathetic nervous system |
| spellingShingle | Antonella VINHOLI Marília Da Cruz FAGUNDES Danieli Cristina PIGOZZO Fernando Bermudez KUBRUSLY Luiz Fernando KUBRUSLY Camila Aparecida Moraes MARQUES INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION ABCD: Arquivos Brasileiros de Cirurgia Digestiva Portal Hypertension Portal vein Ligation Tyrosine 3-monooxygenase Sympathetic nervous system |
| title | INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION |
| title_full | INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION |
| title_fullStr | INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION |
| title_full_unstemmed | INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION |
| title_short | INVOLVEMENT OF CATECHOLAMINES IN THE MYOCARDIUM OF RATS SUBMITTED TO EXPERIMENTAL MODEL OF PORTAL HYPERTENSION |
| title_sort | involvement of catecholamines in the myocardium of rats submitted to experimental model of portal hypertension |
| topic | Portal Hypertension Portal vein Ligation Tyrosine 3-monooxygenase Sympathetic nervous system |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202018000300301&tlng=pt |
| work_keys_str_mv | AT antonellavinholi involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension AT mariliadacruzfagundes involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension AT danielicristinapigozzo involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension AT fernandobermudezkubrusly involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension AT luizfernandokubrusly involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension AT camilaaparecidamoraesmarques involvementofcatecholaminesinthemyocardiumofratssubmittedtoexperimentalmodelofportalhypertension |