The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes
The microbial contamination in the rhizomes of medicinal plants including Curcuma amada rhizomes is generally high. This due to the fact that rhizomes are the bottom parts that grow in the soil. Based on the Regulation of Head of the Indonesian National Agency of Drug and Food Control Number HK.00.0...
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| Format: | Article |
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Center for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN)
2016-08-01
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| Series: | Atom Indonesia |
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| Online Access: | http://aij.batan.go.id/index.php/aij/article/view/508 |
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| author | D.P. Rahayu F.C. Saputri D. Darwis |
| author_facet | D.P. Rahayu F.C. Saputri D. Darwis |
| author_sort | D.P. Rahayu |
| collection | DOAJ |
| description | The microbial contamination in the rhizomes of medicinal plants including Curcuma amada rhizomes is generally high. This due to the fact that rhizomes are the bottom parts that grow in the soil. Based on the Regulation of Head of the Indonesian National Agency of Drug and Food Control Number HK.00.06.1.52.4011, the limits of microbial contamination in herbal/medicinal plants are 106 cfu/g for the total microbial and 2×104 cfu/g for the total yeast and mold. Gamma irradiation is one of the methods to reduce microbial contamination in medicinal plants. In this research, the effectiveness of gamma irradiation in microbial reduction and its effects to curcuminoid contents was determined by irradiating Curcuma amada rhizomes at doses of 5 and 10 kGy. The initial contamination in this rhizome was 8.78×107 cfu/g and 5×101 cfu/g for the total microbial and for the total yeast and mould, respectively. The result indicates that at 5 kGy, the microbial contamination and the mould and yeast contamination were reduced from 8.78×107 cfu/g and 5×101 cfu/g to 1.39×104 cfu/g and under 1×101 cfu/g, respectively. Meanwhile the comparison of curcuminoids between the irradiated and non irradiated samples was performed by HPLC method and was found to actually increase from 0.26% to 0.36% after the 5-kGy irradiation. It can be concluded that an irradiation dose of 5 kGy is effective to reduce the content of microorganisms without lowering curcuminoids. Gamma radiation could be used as decontamination method in medicinal plants. |
| first_indexed | 2024-12-22T08:50:52Z |
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| id | doaj.art-e22a92c68558461b85c4290be2638704 |
| institution | Directory Open Access Journal |
| issn | 0126-1568 0126-1568 |
| language | English |
| last_indexed | 2024-12-22T08:50:52Z |
| publishDate | 2016-08-01 |
| publisher | Center for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN) |
| record_format | Article |
| series | Atom Indonesia |
| spelling | doaj.art-e22a92c68558461b85c4290be26387042022-12-21T18:31:58ZengCenter for Development of Nuclear Informatics, National Nuclear Energy Agency (BATAN)Atom Indonesia0126-15680126-15682016-08-01422535810.17146/aij.2016.508The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. RhizomesD.P. Rahayu0F.C. Saputri1D. Darwis2Center for Isotopes and Radiation Application, National Nuclear Energy Agency, IndonesiaDepartment of Pharmacology, Faculty of Pharmacy, University of Indonesia, Depok 16424, IndonesiaCenter for Isotopes and Radiation Application, National Nuclear Energy Agency, IndonesiaThe microbial contamination in the rhizomes of medicinal plants including Curcuma amada rhizomes is generally high. This due to the fact that rhizomes are the bottom parts that grow in the soil. Based on the Regulation of Head of the Indonesian National Agency of Drug and Food Control Number HK.00.06.1.52.4011, the limits of microbial contamination in herbal/medicinal plants are 106 cfu/g for the total microbial and 2×104 cfu/g for the total yeast and mold. Gamma irradiation is one of the methods to reduce microbial contamination in medicinal plants. In this research, the effectiveness of gamma irradiation in microbial reduction and its effects to curcuminoid contents was determined by irradiating Curcuma amada rhizomes at doses of 5 and 10 kGy. The initial contamination in this rhizome was 8.78×107 cfu/g and 5×101 cfu/g for the total microbial and for the total yeast and mould, respectively. The result indicates that at 5 kGy, the microbial contamination and the mould and yeast contamination were reduced from 8.78×107 cfu/g and 5×101 cfu/g to 1.39×104 cfu/g and under 1×101 cfu/g, respectively. Meanwhile the comparison of curcuminoids between the irradiated and non irradiated samples was performed by HPLC method and was found to actually increase from 0.26% to 0.36% after the 5-kGy irradiation. It can be concluded that an irradiation dose of 5 kGy is effective to reduce the content of microorganisms without lowering curcuminoids. Gamma radiation could be used as decontamination method in medicinal plants.http://aij.batan.go.id/index.php/aij/article/view/508Curcuma amadaGamma irradiationCurcuminoidsDecontamination |
| spellingShingle | D.P. Rahayu F.C. Saputri D. Darwis The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes Atom Indonesia Curcuma amada Gamma irradiation Curcuminoids Decontamination |
| title | The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes |
| title_full | The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes |
| title_fullStr | The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes |
| title_full_unstemmed | The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes |
| title_short | The Effect of Gamma Radiation on Microbial Content and Curcuminoids of Curcuma amada Roxb. Rhizomes |
| title_sort | effect of gamma radiation on microbial content and curcuminoids of curcuma amada roxb rhizomes |
| topic | Curcuma amada Gamma irradiation Curcuminoids Decontamination |
| url | http://aij.batan.go.id/index.php/aij/article/view/508 |
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