Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy
Background: In general, the serum metabolome reflects the patient’s body response to both disease state and implemented treatment. Though serum-derived exosomes are an emerging type of liquid biopsy, the metabolite content of these vesicles remains under researched. The aim of this pilot study was t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/10/4/229 |
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| author | Anna Wojakowska Aneta Zebrowska Agata Skowronek Tomasz Rutkowski Krzysztof Polanski Piotr Widlak Lukasz Marczak Monika Pietrowska |
| author_facet | Anna Wojakowska Aneta Zebrowska Agata Skowronek Tomasz Rutkowski Krzysztof Polanski Piotr Widlak Lukasz Marczak Monika Pietrowska |
| author_sort | Anna Wojakowska |
| collection | DOAJ |
| description | Background: In general, the serum metabolome reflects the patient’s body response to both disease state and implemented treatment. Though serum-derived exosomes are an emerging type of liquid biopsy, the metabolite content of these vesicles remains under researched. The aim of this pilot study was to compare the metabolite profiles of the whole serum and serum-derived exosomes in the context of differences between cancer patients and healthy controls as well as patients’ response to radiotherapy (RT). Methods: Serum samples were collected from 10 healthy volunteers and 10 patients with head and neck cancer before and after RT. Metabolites extracted from serum and exosomes were analyzed by the gas chromatography–mass spectrometry (GC–MS). Results: An untargeted GC–MS-based approach identified 182 and 46 metabolites in serum and exosomes, respectively. Metabolites that differentiated cancer and control samples, either serum or exosomes, were associated with energy metabolism. Serum metabolites affected by RT were associated with the metabolism of amino acids, sugars, lipids, and nucleotides. Conclusions: cancer-related features of energy metabolism could be detected in both types of specimens. On the other hand, in contrast to RT-induced changes observed in serum metabolome, this pilot study did not reveal a specific radiation-related pattern of exosome metabolites. |
| first_indexed | 2024-03-10T14:52:21Z |
| format | Article |
| id | doaj.art-e22d7f70896b4807a8356c9ece0173d2 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T14:52:21Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-e22d7f70896b4807a8356c9ece0173d22023-11-20T20:56:07ZengMDPI AGJournal of Personalized Medicine2075-44262020-11-0110422910.3390/jpm10040229Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by RadiotherapyAnna Wojakowska0Aneta Zebrowska1Agata Skowronek2Tomasz Rutkowski3Krzysztof Polanski4Piotr Widlak5Lukasz Marczak6Monika Pietrowska7Institute of Bioorganic Chemistry Polish Academy of Sciences, 61-704 Poznan, PolandMaria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, PolandMaria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, PolandMaria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, PolandWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UKMaria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, PolandInstitute of Bioorganic Chemistry Polish Academy of Sciences, 61-704 Poznan, PolandMaria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, PolandBackground: In general, the serum metabolome reflects the patient’s body response to both disease state and implemented treatment. Though serum-derived exosomes are an emerging type of liquid biopsy, the metabolite content of these vesicles remains under researched. The aim of this pilot study was to compare the metabolite profiles of the whole serum and serum-derived exosomes in the context of differences between cancer patients and healthy controls as well as patients’ response to radiotherapy (RT). Methods: Serum samples were collected from 10 healthy volunteers and 10 patients with head and neck cancer before and after RT. Metabolites extracted from serum and exosomes were analyzed by the gas chromatography–mass spectrometry (GC–MS). Results: An untargeted GC–MS-based approach identified 182 and 46 metabolites in serum and exosomes, respectively. Metabolites that differentiated cancer and control samples, either serum or exosomes, were associated with energy metabolism. Serum metabolites affected by RT were associated with the metabolism of amino acids, sugars, lipids, and nucleotides. Conclusions: cancer-related features of energy metabolism could be detected in both types of specimens. On the other hand, in contrast to RT-induced changes observed in serum metabolome, this pilot study did not reveal a specific radiation-related pattern of exosome metabolites.https://www.mdpi.com/2075-4426/10/4/229head and neck cancerexosomesserumradiotherapymetabolomicsGC/MS |
| spellingShingle | Anna Wojakowska Aneta Zebrowska Agata Skowronek Tomasz Rutkowski Krzysztof Polanski Piotr Widlak Lukasz Marczak Monika Pietrowska Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy Journal of Personalized Medicine head and neck cancer exosomes serum radiotherapy metabolomics GC/MS |
| title | Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy |
| title_full | Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy |
| title_fullStr | Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy |
| title_full_unstemmed | Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy |
| title_short | Metabolic Profiles of Whole Serum and Serum-Derived Exosomes Are Different in Head and Neck Cancer Patients Treated by Radiotherapy |
| title_sort | metabolic profiles of whole serum and serum derived exosomes are different in head and neck cancer patients treated by radiotherapy |
| topic | head and neck cancer exosomes serum radiotherapy metabolomics GC/MS |
| url | https://www.mdpi.com/2075-4426/10/4/229 |
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