Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers
The present study examined the effects of dietary supplementation with extracts of pomegranate (<i>Punica granatum</i>) and onion (<i>Allium cepa</i>), either encapsulated in cyclodextrin (POMALCD group) or in an aqueous (POMALAQ group) form, on breast meat, thigh meat, and l...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/20/3870 |
| _version_ | 1827720932838342656 |
|---|---|
| author | Soumela Savvidou Nikolas Panteli Vassilios Dotas George Symeon Dimitrios Galamatis Ioannis Panitsidis Eirini Voutsinou Christina Tatidou Prafulla Kumar Efthimia Antonopoulou Georgios Michailidis Ilias Giannenas |
| author_facet | Soumela Savvidou Nikolas Panteli Vassilios Dotas George Symeon Dimitrios Galamatis Ioannis Panitsidis Eirini Voutsinou Christina Tatidou Prafulla Kumar Efthimia Antonopoulou Georgios Michailidis Ilias Giannenas |
| author_sort | Soumela Savvidou |
| collection | DOAJ |
| description | The present study examined the effects of dietary supplementation with extracts of pomegranate (<i>Punica granatum</i>) and onion (<i>Allium cepa</i>), either encapsulated in cyclodextrin (POMALCD group) or in an aqueous (POMALAQ group) form, on breast meat, thigh meat, and liver composition, oxidative stability, cellular signaling pathways, and the gene expression of certain hepatic genes. The results showed that breast and thigh meat contained significantly (<i>p</i> < 0.05) higher moisture content in the group with the aqueous extract, compared to the control and POMALCD groups. Moreover, the protein content was significantly (<i>p</i> < 0.05) higher in the thigh and liver samples of the treated groups in comparison to the control. The iron-induced challenge deteriorated (<i>p</i> < 0.001) the lipid and protein oxidative status of the control group, whereas both supplemented groups showed considerable tolerance in all tissues. The supplementation of pomegranate and onion extracts mitigated or maintained heat shock protein (HSP) levels and elevated (<i>p</i> < 0.05) the Bcl-2/Bad ratio in thigh and breast meat, whereas mitogen-activated protein kinase (MAPK) activation was modulated at a lower rate. After normalization to β-actin expression, quantitative real-time PCR analysis revealed a significant (<i>p</i> < 0.05) induction in the expression of MTR and MSRB1 genes in the liver of the supplemented groups. No differences were observed for the TAT, SMS, and BHMT genes. In conclusion, dietary mixtures of herbal extracts with pomegranate and onion improved protein and lipid oxidative stability in meat, enhanced the hepatic energy status, and exerted ameliorative effects on stress-related proteins. The encapsulated extract of pomegranate and onion, using cyclodextrin as a carrier, appeared to reduce lipid oxidation to a greater extent than the aqueous extract. In contrast, the aqueous extract exhibited higher total antioxidant capacity (TAC) values and provided better protection against protein carbonyl formation. |
| first_indexed | 2024-03-10T21:15:09Z |
| format | Article |
| id | doaj.art-e22f6c18c3b84a8aa095fa71748f3523 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T21:15:09Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-e22f6c18c3b84a8aa095fa71748f35232023-11-19T16:31:00ZengMDPI AGFoods2304-81582023-10-011220387010.3390/foods12203870Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in BroilersSoumela Savvidou0Nikolas Panteli1Vassilios Dotas2George Symeon3Dimitrios Galamatis4Ioannis Panitsidis5Eirini Voutsinou6Christina Tatidou7Prafulla Kumar8Efthimia Antonopoulou9Georgios Michailidis10Ilias Giannenas11Research Institute of Animal Science, Hellenic Agricultural Organization Demeter, 58100 Giannitsa, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Nutrition Physiology & Applied Farm Animal Nutrition, Department of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceResearch Institute of Animal Science, Hellenic Agricultural Organization Demeter, 58100 Giannitsa, GreeceDepartment of Animal Science, School of Agricultural Sciences, University of Thessaly, 41500 Larissa, GreeceLaboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Technical Sales and Research, R&D LifeSciences, 8801 Enterprise Blvd, Largo, FL 33773, USALaboratory of Animal Physiology, Department of Zoology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Physiology of Reproduction of Farm Animals, Department of Animal Production, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Nutrition, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceThe present study examined the effects of dietary supplementation with extracts of pomegranate (<i>Punica granatum</i>) and onion (<i>Allium cepa</i>), either encapsulated in cyclodextrin (POMALCD group) or in an aqueous (POMALAQ group) form, on breast meat, thigh meat, and liver composition, oxidative stability, cellular signaling pathways, and the gene expression of certain hepatic genes. The results showed that breast and thigh meat contained significantly (<i>p</i> < 0.05) higher moisture content in the group with the aqueous extract, compared to the control and POMALCD groups. Moreover, the protein content was significantly (<i>p</i> < 0.05) higher in the thigh and liver samples of the treated groups in comparison to the control. The iron-induced challenge deteriorated (<i>p</i> < 0.001) the lipid and protein oxidative status of the control group, whereas both supplemented groups showed considerable tolerance in all tissues. The supplementation of pomegranate and onion extracts mitigated or maintained heat shock protein (HSP) levels and elevated (<i>p</i> < 0.05) the Bcl-2/Bad ratio in thigh and breast meat, whereas mitogen-activated protein kinase (MAPK) activation was modulated at a lower rate. After normalization to β-actin expression, quantitative real-time PCR analysis revealed a significant (<i>p</i> < 0.05) induction in the expression of MTR and MSRB1 genes in the liver of the supplemented groups. No differences were observed for the TAT, SMS, and BHMT genes. In conclusion, dietary mixtures of herbal extracts with pomegranate and onion improved protein and lipid oxidative stability in meat, enhanced the hepatic energy status, and exerted ameliorative effects on stress-related proteins. The encapsulated extract of pomegranate and onion, using cyclodextrin as a carrier, appeared to reduce lipid oxidation to a greater extent than the aqueous extract. In contrast, the aqueous extract exhibited higher total antioxidant capacity (TAC) values and provided better protection against protein carbonyl formation.https://www.mdpi.com/2304-8158/12/20/3870<i>Punica granatum</i><i>Allium cepa</i>cyclodextrinoxidative stabilitycellular proteinsgene expression |
| spellingShingle | Soumela Savvidou Nikolas Panteli Vassilios Dotas George Symeon Dimitrios Galamatis Ioannis Panitsidis Eirini Voutsinou Christina Tatidou Prafulla Kumar Efthimia Antonopoulou Georgios Michailidis Ilias Giannenas Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers Foods <i>Punica granatum</i> <i>Allium cepa</i> cyclodextrin oxidative stability cellular proteins gene expression |
| title | Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers |
| title_full | Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers |
| title_fullStr | Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers |
| title_full_unstemmed | Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers |
| title_short | Dietary Supplementation with Pomegranate and Onion Affects Lipid and Protein Oxidation in the Breast Meat, Thigh, and Liver, Cellular Stress Protein Responses, and Gene Expression of Liver Enzymes Involved in Protein Synthesis in Broilers |
| title_sort | dietary supplementation with pomegranate and onion affects lipid and protein oxidation in the breast meat thigh and liver cellular stress protein responses and gene expression of liver enzymes involved in protein synthesis in broilers |
| topic | <i>Punica granatum</i> <i>Allium cepa</i> cyclodextrin oxidative stability cellular proteins gene expression |
| url | https://www.mdpi.com/2304-8158/12/20/3870 |
| work_keys_str_mv | AT soumelasavvidou dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT nikolaspanteli dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT vassiliosdotas dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT georgesymeon dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT dimitriosgalamatis dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT ioannispanitsidis dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT eirinivoutsinou dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT christinatatidou dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT prafullakumar dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT efthimiaantonopoulou dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT georgiosmichailidis dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers AT iliasgiannenas dietarysupplementationwithpomegranateandonionaffectslipidandproteinoxidationinthebreastmeatthighandlivercellularstressproteinresponsesandgeneexpressionofliverenzymesinvolvedinproteinsynthesisinbroilers |