Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection
Persistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an i...
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MDPI AG
2020-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/10/842 |
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| author | Hieu Van Dong Giang Thi Huong Tran Dai Quang Trinh Yohei Takeda Haruko Ogawa Kunitoshi Imai |
| author_facet | Hieu Van Dong Giang Thi Huong Tran Dai Quang Trinh Yohei Takeda Haruko Ogawa Kunitoshi Imai |
| author_sort | Hieu Van Dong |
| collection | DOAJ |
| description | Persistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an in vitro model of persistent CAV infection. CAV-infected MDCC-MSB1 (MSB1) cells, a Marek’s disease virus-transformed continuous cell line, were cultured in the presence of both CAV and CAV neutralizing antibody (NA). Cell viability, expression of viral antigens, viral DNA, and recovery of CAV were examined by acridine orange/propidium iodide staining, immunofluorescence measurement, real-time PCR, and viral isolation, respectively. The results indicated that CAV was maintained and possibly replicated in CAV-infected cells cultured in the presence of NA, without affecting host cell viability. It was also shown that persistently infectious CAV induced cell death again after removing NA. The persistent infection of CAV in MSB1 cells was not related to viral gene mutation. In summary, we have herein established a novel model of persistent CAV infection in MSB1 cells cultured in the presence of NA. |
| first_indexed | 2024-03-10T15:37:57Z |
| format | Article |
| id | doaj.art-e22fa5b079254502ac3a567573d5bffd |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T15:37:57Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-e22fa5b079254502ac3a567573d5bffd2023-11-20T17:09:25ZengMDPI AGPathogens2076-08172020-10-0191084210.3390/pathogens9100842Establishment of an In Vitro Model of Persistent Chicken Anemia Virus InfectionHieu Van Dong0Giang Thi Huong Tran1Dai Quang Trinh2Yohei Takeda3Haruko Ogawa4Kunitoshi Imai5United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, JapanUnited Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, JapanCentral Veterinary Medicine JSC No. 5, Ha Binh Phuong Industrial Zone, Thuong Tin, Hanoi 131000, VietnamResearch Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanUnited Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City 501-1193, JapanDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanPersistent infection of chicken anemia virus (CAV) in chickens has been suspected to result in immunosuppression and exogenous virus contamination within vaccine production. However, no direct evidence for persistent CAV infection has thus far been obtained. In this study, we aimed to establish an in vitro model of persistent CAV infection. CAV-infected MDCC-MSB1 (MSB1) cells, a Marek’s disease virus-transformed continuous cell line, were cultured in the presence of both CAV and CAV neutralizing antibody (NA). Cell viability, expression of viral antigens, viral DNA, and recovery of CAV were examined by acridine orange/propidium iodide staining, immunofluorescence measurement, real-time PCR, and viral isolation, respectively. The results indicated that CAV was maintained and possibly replicated in CAV-infected cells cultured in the presence of NA, without affecting host cell viability. It was also shown that persistently infectious CAV induced cell death again after removing NA. The persistent infection of CAV in MSB1 cells was not related to viral gene mutation. In summary, we have herein established a novel model of persistent CAV infection in MSB1 cells cultured in the presence of NA.https://www.mdpi.com/2076-0817/9/10/842chicken anemia virusin vitro modelneutralizing antibodypersistent infection |
| spellingShingle | Hieu Van Dong Giang Thi Huong Tran Dai Quang Trinh Yohei Takeda Haruko Ogawa Kunitoshi Imai Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection Pathogens chicken anemia virus in vitro model neutralizing antibody persistent infection |
| title | Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection |
| title_full | Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection |
| title_fullStr | Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection |
| title_full_unstemmed | Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection |
| title_short | Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection |
| title_sort | establishment of an in vitro model of persistent chicken anemia virus infection |
| topic | chicken anemia virus in vitro model neutralizing antibody persistent infection |
| url | https://www.mdpi.com/2076-0817/9/10/842 |
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