Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta
The β-1,3-glucan from yeast has been extensively examined for its immuno-enhancing effects in animals. However, investigation on the relationship among β-glucan, gut microbiota and immune-modulating effects remains limited particularly in pigs. Considering the critical roles of gut methanogens in th...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-12-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913603810 |
| _version_ | 1818739418007076864 |
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| author | Yu-heng LUO Hua LI Jun-qiu LUO Ke-ying ZHANG |
| author_facet | Yu-heng LUO Hua LI Jun-qiu LUO Ke-ying ZHANG |
| author_sort | Yu-heng LUO |
| collection | DOAJ |
| description | The β-1,3-glucan from yeast has been extensively examined for its immuno-enhancing effects in animals. However, investigation on the relationship among β-glucan, gut microbiota and immune-modulating effects remains limited particularly in pigs. Considering the critical roles of gut methanogens in the microbial fermentation, energy metabolism and disease resistance, we investigated the phylogenetic diversity of methanogens from fermented cultures of porcine colonic digesta with (G) or without (N) yeast β-glucan based on sequences of the archaeal 16S rRNA gene. A total of 145 sequences in the G library were assigned into 8 operational taxonomic units (OTUs) with the majority of sequences (114/145) related to strains Methanobrevibacter millerae or Methanobrevibacter gottschalkii with high identities ranging from 97.9 to 98.6%, followed by 23 sequences to Methanobrevibacter ruminantium, 2 sequences to Methanobrevibacter smithii and one sequence to Methanobrevibacter wolinii. The 142 sequences in the N library were assigned to 2 OTUs with most sequences (127/142) related to strains M. millerae or M. gottschalkii with sequence identities ranging from 97.9 to 98.5%, and 15 sequences related to M. gottschalkii with 97.9% identity. Shannon diversity index showed that the G library exhibited significantly higher archaeal diversity (P<0.05) and Libshuff analysis indicated the differences in the community structure between the two libraries were significant (P<0.0001). In conclusion, the current study provides evidence that addition of yeast β-glucan significantly increased the diversity of methanogens in in vitro fermented porcine colonic digesta. |
| first_indexed | 2024-12-18T01:24:31Z |
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| id | doaj.art-e231beec91164b198339ee936f2e332f |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-18T01:24:31Z |
| publishDate | 2013-12-01 |
| publisher | Elsevier |
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| series | Journal of Integrative Agriculture |
| spelling | doaj.art-e231beec91164b198339ee936f2e332f2022-12-21T21:25:46ZengElsevierJournal of Integrative Agriculture2095-31192013-12-01121222292234Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic DigestaYu-heng LUO0Hua LI1Jun-qiu LUO2Ke-ying ZHANG3Correspondence LUO Yu-heng, Tel: +86-835-2885269; Key Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province and China Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R. ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province and China Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R. ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province and China Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R. ChinaKey Laboratory for Animal Disease-Resistance Nutrition of Sichuan Province and China Ministry of Education/Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, P.R. ChinaThe β-1,3-glucan from yeast has been extensively examined for its immuno-enhancing effects in animals. However, investigation on the relationship among β-glucan, gut microbiota and immune-modulating effects remains limited particularly in pigs. Considering the critical roles of gut methanogens in the microbial fermentation, energy metabolism and disease resistance, we investigated the phylogenetic diversity of methanogens from fermented cultures of porcine colonic digesta with (G) or without (N) yeast β-glucan based on sequences of the archaeal 16S rRNA gene. A total of 145 sequences in the G library were assigned into 8 operational taxonomic units (OTUs) with the majority of sequences (114/145) related to strains Methanobrevibacter millerae or Methanobrevibacter gottschalkii with high identities ranging from 97.9 to 98.6%, followed by 23 sequences to Methanobrevibacter ruminantium, 2 sequences to Methanobrevibacter smithii and one sequence to Methanobrevibacter wolinii. The 142 sequences in the N library were assigned to 2 OTUs with most sequences (127/142) related to strains M. millerae or M. gottschalkii with sequence identities ranging from 97.9 to 98.5%, and 15 sequences related to M. gottschalkii with 97.9% identity. Shannon diversity index showed that the G library exhibited significantly higher archaeal diversity (P<0.05) and Libshuff analysis indicated the differences in the community structure between the two libraries were significant (P<0.0001). In conclusion, the current study provides evidence that addition of yeast β-glucan significantly increased the diversity of methanogens in in vitro fermented porcine colonic digesta.http://www.sciencedirect.com/science/article/pii/S2095311913603810β-glucanmethanogendiversitypigcolonic digestain vitro |
| spellingShingle | Yu-heng LUO Hua LI Jun-qiu LUO Ke-ying ZHANG Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta Journal of Integrative Agriculture β-glucan methanogen diversity pig colonic digesta in vitro |
| title | Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta |
| title_full | Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta |
| title_fullStr | Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta |
| title_full_unstemmed | Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta |
| title_short | Yeast-Derived β-1,3-Glucan Substrate Significantly Increased the Diversity of Methanogens During In vitro Fermentation of Porcine Colonic Digesta |
| title_sort | yeast derived β 1 3 glucan substrate significantly increased the diversity of methanogens during in vitro fermentation of porcine colonic digesta |
| topic | β-glucan methanogen diversity pig colonic digesta in vitro |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913603810 |
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