Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats
Abstract Background The Thai traditional herbal formula–Mathurameha, consisting of 26 medicinal plants, has been used as an alternative and complementary medicine for diabetes treatment in Wangnamyen Hospital, Thailand. To provide scientific evidences on the efficacy and safety of this herbal formul...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1851-8 |
| _version_ | 1819103779296903168 |
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| author | Kullacha Chayarop Penchom Peungvicha Rungravi Temsiririrkkul Yuvadee Wongkrajang Wongsatit Chuakul Piyanuch Rojsanga |
| author_facet | Kullacha Chayarop Penchom Peungvicha Rungravi Temsiririrkkul Yuvadee Wongkrajang Wongsatit Chuakul Piyanuch Rojsanga |
| author_sort | Kullacha Chayarop |
| collection | DOAJ |
| description | Abstract Background The Thai traditional herbal formula–Mathurameha, consisting of 26 medicinal plants, has been used as an alternative and complementary medicine for diabetes treatment in Wangnamyen Hospital, Thailand. To provide scientific evidences on the efficacy and safety of this herbal formula, in vivo hypoglycaemic activity, effect on serum biochemical profiles and acute toxicity were investigated. Methods Experimental type 2 diabetes was induced in male Sprague-Dawley rats by intraperitoneal injection of nicotinamide 15 min prior to intravenous injection of streptozotocin. The most effective extract from the oral glucose tolerant test (OGTT) was administered daily via the oral route to diabetic rats for 2 weeks. Two-hour postprandial plasma glucose (2h–PPG) levels were measured on days 0, 7, and 14. Biochemical data were measured at the end of daily oral administration experiment. Results Aqueous extract of the herbal formula was the most potent extract for improving glucose tolerance of streptozotocin-nicotinamide-induced diabetic rats after single oral administration. After 2 weeks of daily oral administration, the aqueous extract showed a dose-dependent glucose lowering effect. At doses of 12.5, 25, and 50 mg/kg, the 2h–PPG level of diabetic rats decreased by 3.32%, 15.78%, and 17.94%, respectively. Most of the biochemical profiles of diabetic rats were improved, including the total cholesterol (TC), alkaline phosphatase (ALP), total protein, albumin, globulin, creatinine, and uric acid levels. The significantly increased triglyceride (TG) level observed in treated diabetic rats indicated a lack of a beneficial effect of the extract on lipid homeostasis. Nevertheless, there were no signs or symptoms of acute toxicity observed after oral administration of aqueous extract (5 g/kg) to both male and female rats. Conclusions The results revealed that the herbal formula aqueous extract has hypoglycaemic activity, beneficial effects on biochemical profiles and a lack of acute toxicity. This study confirms the efficacy and safety of the Mathurameha herbal formula used for treating type 2 diabetes mellitus. |
| first_indexed | 2024-12-22T01:55:53Z |
| format | Article |
| id | doaj.art-e2327e447d0742c2b686460b0fc78250 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T01:55:53Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-e2327e447d0742c2b686460b0fc782502022-12-21T18:42:47ZengBMCBMC Complementary and Alternative Medicine1472-68822017-06-0117111110.1186/s12906-017-1851-8Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic ratsKullacha Chayarop0Penchom Peungvicha1Rungravi Temsiririrkkul2Yuvadee Wongkrajang3Wongsatit Chuakul4Piyanuch Rojsanga5Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol UniversityDepartment of Physiology, Faculty of Pharmacy, Mahidol UniversityDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol UniversityDepartment of Physiology, Faculty of Pharmacy, Mahidol UniversityDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol UniversityDepartment of Pharmaceutical Chemistry, Mahidol UniversityAbstract Background The Thai traditional herbal formula–Mathurameha, consisting of 26 medicinal plants, has been used as an alternative and complementary medicine for diabetes treatment in Wangnamyen Hospital, Thailand. To provide scientific evidences on the efficacy and safety of this herbal formula, in vivo hypoglycaemic activity, effect on serum biochemical profiles and acute toxicity were investigated. Methods Experimental type 2 diabetes was induced in male Sprague-Dawley rats by intraperitoneal injection of nicotinamide 15 min prior to intravenous injection of streptozotocin. The most effective extract from the oral glucose tolerant test (OGTT) was administered daily via the oral route to diabetic rats for 2 weeks. Two-hour postprandial plasma glucose (2h–PPG) levels were measured on days 0, 7, and 14. Biochemical data were measured at the end of daily oral administration experiment. Results Aqueous extract of the herbal formula was the most potent extract for improving glucose tolerance of streptozotocin-nicotinamide-induced diabetic rats after single oral administration. After 2 weeks of daily oral administration, the aqueous extract showed a dose-dependent glucose lowering effect. At doses of 12.5, 25, and 50 mg/kg, the 2h–PPG level of diabetic rats decreased by 3.32%, 15.78%, and 17.94%, respectively. Most of the biochemical profiles of diabetic rats were improved, including the total cholesterol (TC), alkaline phosphatase (ALP), total protein, albumin, globulin, creatinine, and uric acid levels. The significantly increased triglyceride (TG) level observed in treated diabetic rats indicated a lack of a beneficial effect of the extract on lipid homeostasis. Nevertheless, there were no signs or symptoms of acute toxicity observed after oral administration of aqueous extract (5 g/kg) to both male and female rats. Conclusions The results revealed that the herbal formula aqueous extract has hypoglycaemic activity, beneficial effects on biochemical profiles and a lack of acute toxicity. This study confirms the efficacy and safety of the Mathurameha herbal formula used for treating type 2 diabetes mellitus.http://link.springer.com/article/10.1186/s12906-017-1851-8MathuramehaThai traditional medicineHerbal formulaDiabetes mellitusHypoglycaemic activityAcute toxicity |
| spellingShingle | Kullacha Chayarop Penchom Peungvicha Rungravi Temsiririrkkul Yuvadee Wongkrajang Wongsatit Chuakul Piyanuch Rojsanga Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats BMC Complementary and Alternative Medicine Mathurameha Thai traditional medicine Herbal formula Diabetes mellitus Hypoglycaemic activity Acute toxicity |
| title | Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats |
| title_full | Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats |
| title_fullStr | Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats |
| title_full_unstemmed | Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats |
| title_short | Hypoglycaemic activity of Mathurameha, a Thai traditional herbal formula aqueous extract, and its effect on biochemical profiles of streptozotocin-nicotinamide-induced diabetic rats |
| title_sort | hypoglycaemic activity of mathurameha a thai traditional herbal formula aqueous extract and its effect on biochemical profiles of streptozotocin nicotinamide induced diabetic rats |
| topic | Mathurameha Thai traditional medicine Herbal formula Diabetes mellitus Hypoglycaemic activity Acute toxicity |
| url | http://link.springer.com/article/10.1186/s12906-017-1851-8 |
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