Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer
Selecting a well-suited method for isolating/characterizing circulating tumor cells (CTCs) is challenging. Evaluating sensitive and specific markers for prostate cancer (PCa)-specific CTC identification and analysis is crucial. We used the CellCollector EpCAM-functionalized system (CC-EpCAM) and eva...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/2/497 |
| _version_ | 1797481181552312320 |
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| author | Gerit Theil Joanna Bialek Christine Weiß Felix Lindner Paolo Fornara |
| author_facet | Gerit Theil Joanna Bialek Christine Weiß Felix Lindner Paolo Fornara |
| author_sort | Gerit Theil |
| collection | DOAJ |
| description | Selecting a well-suited method for isolating/characterizing circulating tumor cells (CTCs) is challenging. Evaluating sensitive and specific markers for prostate cancer (PCa)-specific CTC identification and analysis is crucial. We used the CellCollector EpCAM-functionalized system (CC-EpCAM) and evaluated and developed a PCa-functionalized version (CC-PCa); we then compared CTC isolation techniques that exploit the physical and biological properties of CTCs. We established two cohorts of metastatic PCa patients (mPCa; 15 in cohort 1 and 10 in cohort 2). CTC cultivation experiments were conducted with two capturing methods (Ficoll and ScreenCell). The most sensitive detection rates and highest CTC counts were reached with the CC-PCa and ScreenCell system. Patients with ≥5 CTCs isolated with CC-EpCAM had an overall survival (OS) of 0.93 years, and patients with ≥5 CTCs isolated with CC-PCa had an OS of 1.5 years in cohort 1. Nevertheless, we observed the highest sensitivity and specificity for 24-month survival by the Ficoll with CD45 depletion and ScreenCell system with May-Grunwald Giemsa (MGG) staining. The EpCAM molecule is an essential factor related to OS for CTC isolation based on biological properties in mPCa patients. The best-suited CTC capture system is not limited to one characteristic of cells but adapted to downstream analysis. |
| first_indexed | 2024-03-09T22:10:50Z |
| format | Article |
| id | doaj.art-e2327eb4a6d94cb08ffa638334d0f650 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T22:10:50Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-e2327eb4a6d94cb08ffa638334d0f6502023-11-23T19:33:07ZengMDPI AGDiagnostics2075-44182022-02-0112249710.3390/diagnostics12020497Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate CancerGerit Theil0Joanna Bialek1Christine Weiß2Felix Lindner3Paolo Fornara4Medical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), GermanyMedical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), GermanyMedical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), GermanyMedical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), GermanyMedical Faculty of Martin Luther University Halle-Wittenberg, University Clinic and Outpatient Clinic for Urology, 06120 Halle (Saale), GermanySelecting a well-suited method for isolating/characterizing circulating tumor cells (CTCs) is challenging. Evaluating sensitive and specific markers for prostate cancer (PCa)-specific CTC identification and analysis is crucial. We used the CellCollector EpCAM-functionalized system (CC-EpCAM) and evaluated and developed a PCa-functionalized version (CC-PCa); we then compared CTC isolation techniques that exploit the physical and biological properties of CTCs. We established two cohorts of metastatic PCa patients (mPCa; 15 in cohort 1 and 10 in cohort 2). CTC cultivation experiments were conducted with two capturing methods (Ficoll and ScreenCell). The most sensitive detection rates and highest CTC counts were reached with the CC-PCa and ScreenCell system. Patients with ≥5 CTCs isolated with CC-EpCAM had an overall survival (OS) of 0.93 years, and patients with ≥5 CTCs isolated with CC-PCa had an OS of 1.5 years in cohort 1. Nevertheless, we observed the highest sensitivity and specificity for 24-month survival by the Ficoll with CD45 depletion and ScreenCell system with May-Grunwald Giemsa (MGG) staining. The EpCAM molecule is an essential factor related to OS for CTC isolation based on biological properties in mPCa patients. The best-suited CTC capture system is not limited to one characteristic of cells but adapted to downstream analysis.https://www.mdpi.com/2075-4418/12/2/497circulating tumor cellsprostate cancerisolation platforms |
| spellingShingle | Gerit Theil Joanna Bialek Christine Weiß Felix Lindner Paolo Fornara Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer Diagnostics circulating tumor cells prostate cancer isolation platforms |
| title | Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer |
| title_full | Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer |
| title_fullStr | Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer |
| title_full_unstemmed | Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer |
| title_short | Strategies for Isolating and Propagating Circulating Tumor Cells in Men with Metastatic Prostate Cancer |
| title_sort | strategies for isolating and propagating circulating tumor cells in men with metastatic prostate cancer |
| topic | circulating tumor cells prostate cancer isolation platforms |
| url | https://www.mdpi.com/2075-4418/12/2/497 |
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