Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells
The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore iden...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2018-03-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/20808 |
| _version_ | 1811159012514725888 |
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| author | Silmi Mariya Fitriya Nur Annisa Dewi Irma Herawati Suparto Gregory K. Wilkerson J. Mark Cline . Permanawati Diah Iskandriati I Nengah Budiarsa Dondin Sajuthi |
| author_facet | Silmi Mariya Fitriya Nur Annisa Dewi Irma Herawati Suparto Gregory K. Wilkerson J. Mark Cline . Permanawati Diah Iskandriati I Nengah Budiarsa Dondin Sajuthi |
| author_sort | Silmi Mariya |
| collection | DOAJ |
| description | The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24, CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future. |
| first_indexed | 2024-04-10T05:33:58Z |
| format | Article |
| id | doaj.art-e2328440deae45e1863243830256414b |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-10T05:33:58Z |
| publishDate | 2018-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-e2328440deae45e1863243830256414b2023-03-07T02:22:07ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942018-03-0124310.4308/hjb.24.3.136Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem CellsSilmi Mariya0Fitriya Nur Annisa Dewi1Irma Herawati Suparto2Gregory K. Wilkerson3J. Mark Cline4. Permanawati5Diah Iskandriati6I Nengah Budiarsa7Dondin Sajuthi8Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Michale E. Keeling Center for Comparative Medicine and Research, MD Anderson Cancer Center, Bastrop, TXWake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24, CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future.https://journal.ipb.ac.id/index.php/hayati/article/view/20808breast cancermammary glandnonhuman primatestem cells |
| spellingShingle | Silmi Mariya Fitriya Nur Annisa Dewi Irma Herawati Suparto Gregory K. Wilkerson J. Mark Cline . Permanawati Diah Iskandriati I Nengah Budiarsa Dondin Sajuthi Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells Hayati Journal of Biosciences breast cancer mammary gland nonhuman primate stem cells |
| title | Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells |
| title_full | Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells |
| title_fullStr | Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells |
| title_full_unstemmed | Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells |
| title_short | Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells |
| title_sort | mammary gland cell culture of macaca fascicularis as a reservoir for stem cells |
| topic | breast cancer mammary gland nonhuman primate stem cells |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/20808 |
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