Quantitative PCR assays to detect whales, rockfish, and common murre environmental DNA in marine water samples of the Northeastern Pacific.

Monitoring aquatic species by identification of environmental DNA (eDNA) is becoming more common. To obtain quantitative eDNA datasets for individual species, organism-specific quantitative PCR (qPCR) assays are required. Here, we present detailed methodology of qPCR assay design and testing, includ...

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Autors principals: Elizabeth A Andruszkiewicz, Kevan M Yamahara, Collin J Closek, Alexandria B Boehm
Format: Article
Idioma:English
Publicat: Public Library of Science (PLoS) 2020-01-01
Col·lecció:PLoS ONE
Accés en línia:https://doi.org/10.1371/journal.pone.0242689