Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras
BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and r...
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100009&lng=en&tlng=en |
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| author | Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen |
| author_facet | Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen |
| author_sort | Osbourne Quaye |
| collection | DOAJ |
| description | BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain. METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN CONCLUSIONS The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions. |
| first_indexed | 2024-03-12T08:50:22Z |
| format | Article |
| id | doaj.art-e2356345d7204891b1b3b8342b89ba94 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:50:22Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-e2356345d7204891b1b3b8342b89ba942023-09-02T16:21:41ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-8060113191610.1590/0074-02760170083S0074-02762018000100009Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in HondurasOsbourne QuayeSunando RoyKunchala RungsrisuriyachaiMathew D EsonaZiqian XuKa Ian TamDina J Castro BanegasGloria Rey-BenitoMichael D BowenBACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains. OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011 rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was characterised to determine the possible evolutionary origin of the rare strain. METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3, NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain. MAIN CONCLUSIONS The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from multiple reassortment events which were possibly mediated through interspecies transmissions.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100009&lng=en&tlng=enrotavirusviral genomeviral proteinsnon-structural proteinsreassortant |
| spellingShingle | Osbourne Quaye Sunando Roy Kunchala Rungsrisuriyachai Mathew D Esona Ziqian Xu Ka Ian Tam Dina J Castro Banegas Gloria Rey-Benito Michael D Bowen Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras Memorias do Instituto Oswaldo Cruz rotavirus viral genome viral proteins non-structural proteins reassortant |
| title | Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
| title_full | Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
| title_fullStr | Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
| title_full_unstemmed | Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
| title_short | Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras |
| title_sort | characterisation of a rare reassortant human g10p 14 rotavirus strain detected in honduras |
| topic | rotavirus viral genome viral proteins non-structural proteins reassortant |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000100009&lng=en&tlng=en |
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