Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines
Human rhinoviruses (HRVs) are small non-enveloped RNA viruses that belong to the Enterovirus genus within the Picornaviridae family and are known for causing the common cold. Though symptoms are generally mild in healthy individuals, the economic burden associated with HRV infection is significant....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/14/10/2235 |
| _version_ | 1797469663824707584 |
|---|---|
| author | Wouter Johannes Petrus van den Braak Bella Monica Diana Limpens Dedeke Rockx-Brouwer Matthijn de Boer Dinja Oosterhoff |
| author_facet | Wouter Johannes Petrus van den Braak Bella Monica Diana Limpens Dedeke Rockx-Brouwer Matthijn de Boer Dinja Oosterhoff |
| author_sort | Wouter Johannes Petrus van den Braak |
| collection | DOAJ |
| description | Human rhinoviruses (HRVs) are small non-enveloped RNA viruses that belong to the Enterovirus genus within the Picornaviridae family and are known for causing the common cold. Though symptoms are generally mild in healthy individuals, the economic burden associated with HRV infection is significant. A vaccine could prevent disease. The Vero-cell-based viral vaccine platform technology was considered for such vaccine development. Unfortunately, most HRV strains are unable to propagate on Vero cells due to a lack of the major receptor of HRV group A and B, intercellular adhesion molecule (ICAM1, also known as CD54). Therefore, stable human ICAM1 expressing Vero cell clones were generated by transfecting the ICAM1 gene in Vero cells and selecting clones that overexpressed ICAM1 on the cell surface. Cell banks were made and expression of ICAM1 was stable for at least 30 passages. The Vero_ICAM1 cells and parental Vero cells were infected with four HRV prototypes, B14, A16, B37 and A57. Replication of all four viruses was detected in Vero_ICAM1, but not in the parental Vero cells. Altogether, Vero cells expressing ICAM1 could efficiently propagate the tested HRV strains. Therefore, ICAM1-expressing cells could be a useful tool for the development and future production of polyvalent HRV vaccines or other viruses that use ICAM1 as a receptor. |
| first_indexed | 2024-03-09T19:23:23Z |
| format | Article |
| id | doaj.art-e2379990610a4fbaa628cf09947247db |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T19:23:23Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-e2379990610a4fbaa628cf09947247db2023-11-24T03:09:55ZengMDPI AGViruses1999-49152022-10-011410223510.3390/v14102235Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus VaccinesWouter Johannes Petrus van den Braak0Bella Monica1Diana Limpens2Dedeke Rockx-Brouwer3Matthijn de Boer4Dinja Oosterhoff5Intravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsIntravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsIntravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsIntravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsIntravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsIntravacc, P.O. Box 450, 3720 AL Bilthoven, The NetherlandsHuman rhinoviruses (HRVs) are small non-enveloped RNA viruses that belong to the Enterovirus genus within the Picornaviridae family and are known for causing the common cold. Though symptoms are generally mild in healthy individuals, the economic burden associated with HRV infection is significant. A vaccine could prevent disease. The Vero-cell-based viral vaccine platform technology was considered for such vaccine development. Unfortunately, most HRV strains are unable to propagate on Vero cells due to a lack of the major receptor of HRV group A and B, intercellular adhesion molecule (ICAM1, also known as CD54). Therefore, stable human ICAM1 expressing Vero cell clones were generated by transfecting the ICAM1 gene in Vero cells and selecting clones that overexpressed ICAM1 on the cell surface. Cell banks were made and expression of ICAM1 was stable for at least 30 passages. The Vero_ICAM1 cells and parental Vero cells were infected with four HRV prototypes, B14, A16, B37 and A57. Replication of all four viruses was detected in Vero_ICAM1, but not in the parental Vero cells. Altogether, Vero cells expressing ICAM1 could efficiently propagate the tested HRV strains. Therefore, ICAM1-expressing cells could be a useful tool for the development and future production of polyvalent HRV vaccines or other viruses that use ICAM1 as a receptor.https://www.mdpi.com/1999-4915/14/10/2235rhinovirusintercellular adhesion molecule 1ICAM1Vero cellmanufacturing cell line |
| spellingShingle | Wouter Johannes Petrus van den Braak Bella Monica Diana Limpens Dedeke Rockx-Brouwer Matthijn de Boer Dinja Oosterhoff Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines Viruses rhinovirus intercellular adhesion molecule 1 ICAM1 Vero cell manufacturing cell line |
| title | Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines |
| title_full | Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines |
| title_fullStr | Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines |
| title_full_unstemmed | Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines |
| title_short | Construction of a Vero Cell Line Expressing Human ICAM1 for the Development of Rhinovirus Vaccines |
| title_sort | construction of a vero cell line expressing human icam1 for the development of rhinovirus vaccines |
| topic | rhinovirus intercellular adhesion molecule 1 ICAM1 Vero cell manufacturing cell line |
| url | https://www.mdpi.com/1999-4915/14/10/2235 |
| work_keys_str_mv | AT wouterjohannespetrusvandenbraak constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines AT bellamonica constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines AT dianalimpens constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines AT dedekerockxbrouwer constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines AT matthijndeboer constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines AT dinjaoosterhoff constructionofaverocelllineexpressinghumanicam1forthedevelopmentofrhinovirusvaccines |